Abstract:
:The epitopes of about 100 monoclonal antibodies against human type II DNA topoisomerase were mapped along the enzyme molecules. Although they were randomly and independently established, epitope sites were unevenly distributed the toward N-terminal or C-terminal region. We suggest that the central catalytic domain is hidden inside the molecule and inaccessible to the antigen recognition sites. Using antibodies, we demonstrate the distinct localization of isoforms of Topo II in cultured cells. Some particularly useful antibodies are listed.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Sakaguchi A,Miyaike M,Kuroda K,Nozaki N,Tanaka M,Hibino M,Fujii Y,Kato S,Kikuchi Adoi
10.1093/oxfordjournals.jbchem.a003237keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
409-16issue
3eissn
0021-924Xissn
1756-2651journal_volume
132pub_type
杂志文章abstract::A novel phenylserine dehydratase [EC 4.2.1.-], which catalyzes the deamination of L-threo-3-phenylserine to yield phenylpyruvate and ammonia, was purified to homogeneity from a crude extract of Pseudomonas pickettii PS22 isolated from soil. The enzyme was a monomer having a molecular mass of about 38 kDa and contained...
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更新日期:1989-05-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134883
更新日期:1984-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131363
更新日期:1976-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123277
更新日期:1990-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131135
更新日期:1976-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133734
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133877
更新日期:1982-05-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:2017-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124617
更新日期:1994-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1977-12-01 00:00:00
abstract::The E2F transcription factors (TFs), which control the progression of the cell cycle in response to DNA-damage and various stresses, are known to interact with a tumor suppressor, Retinoblastoma 1 (RB1). We previously showed that the response of the human RB1 promoter to a 12-O-tetradecanoylphorbol-13-acetate (TPA) in...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2019-06-14 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021739
更新日期:1997-07-01 00:00:00