Inhibition of eukaryotic DNA polymerase-alpha by polydeoxynucleotides.

abstract：:The effect of pyocin R1 on the glucose metabolism of sensitive Pseudomonas cells was investigated. Upon treatment with pyocin R1, although the rate of O2 uptake of the sensitive cells for glucose or gluconate was not very much affected at first, the final level of O2 uptake was greatly reduced. When 2-oxogluconate was...

journal_title：Journal of biochemistry

authors： Kageyama M

更新日期：1978-12-01 00:00:00

abstract：:Rhodopsin from the bovine rod outer segment contains a covalently linked carbohydrate moiety (Heller, J. & Lawrence, M.A. (1973) Biochemistry 9, 864--868). We studied the location of this carbohydrate moiety on the disk membrane by using ferritin-conjugated concanavalin A and concanavalin A labelled with fluorescein i...

journal_title：Journal of biochemistry

authors： Nakano T,Ikai A,Nishigai M,Noda H

更新日期：1979-05-01 00:00:00

abstract：:The physicochemical nature of an active fragment (T2A) of colicin E3 was examined together with those of its specific inhibitor, B, and the T2A-B complex under various conditions by means of CD and fluorescence spectroscopy. 1. T2A was mainly composed of unordered structure, although the molecule was folded into a rat...

journal_title：Journal of biochemistry

authors： Suzuki K,Ohno S,Imahori K

更新日期：1980-03-01 00:00:00

abstract：:Hyperthermophilic bacteria Thermotoga maritima and Thermotoga hypogea produce ethanol as a metabolic end product, which is resulted from acetaldehyde reduction catalysed by an alcohol dehydrogenase (ADH). However, the enzyme that is involved in the production of acetaldehyde from pyruvate is not well characterized. An...

journal_title：Journal of biochemistry

authors： Eram MS,Wong A,Oduaran E,Ma K

更新日期：2015-12-01 00:00:00

abstract：:Using an F-actin affinity column, a 60 kDa fragment of a 71 kDa F-actin-binding protein was partially purified from Tetrahymena pyriformis. After digestion of the 60 kDa fragment with cyanogen bromide, the N-terminal 21-amino acid sequence of one of the resulting peptides was found to show sequence similarity to a reg...

journal_title：Journal of biochemistry

authors： Watanabe A,Kurasawa Y,Watanabe Y,Numata O

更新日期：1998-04-01 00:00:00

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:The amino acid sequence of glutathione S-transferase a from guinea pig liver was determined. Glutathione S-transferase a was composed of two identical subunits, each comprising 218 amino acid residues. The amino acid sequence of glutathione S-transferase a exhibited 73% homology with that of human glutathione S-transf...

journal_title：Journal of biochemistry

authors： Kamei-Hayashi K,Oshino R,Hara S

更新日期：1993-12-01 00:00:00

abstract：:Three radioactive glycopeptides were isolated from human peripheral lymphocytes stimulated with Wistaria floribunda mitogen in the presence of D-[14C]glucosamine hydrochloride by mild trypsin digestion followed by gel filtration and preparative high-voltage paper electrophoresis. The carbohydrate compositions of these...

journal_title：Journal of biochemistry

authors： Saito M,Toyoshima S,Osawa T

更新日期：1977-05-01 00:00:00

abstract：:Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

journal_title：Journal of biochemistry

authors： Kiyonari S,Egashira Y,Ishino S,Ishino Y

更新日期：2014-05-01 00:00:00

abstract：:Three forms of cytochrome P-450 of liver microsomes of 3-methylcholanthrene-treated Golden hamsters were purified and characterized as regards their catalytic activity toward aflatoxin B1-related hepatocarcinogenic mycotoxins. These include two major forms, designated as cytochrome P-450-AFB (P-450-I) and P-450-II, an...

journal_title：Journal of biochemistry

authors： Fukuhara M,Nohmi T,Mizokami K,Sunouchi M,Ishidate M Jr,Takanaka A

更新日期：1989-08-01 00:00:00

abstract：:Cytokine-induced neutrophil chemoattractant (CINC/Gro) is a chemotactic factor for neutrophils in the rat and a member of the CXC chemokine family. The refined three-dimensional structure of CINC/Gro was derived with the aid of heteronuclear magnetic resonance spectroscopy and a hybrid method of distance geometry and ...

journal_title：Journal of biochemistry

authors： Hanzawa H,Haruyama H,Konishi K,Watanabe K,Tsurufuji S

更新日期：1998-01-01 00:00:00

abstract：:Puromycin-mediated in vitro release of nascent peptides from rat liver ribosomes was significantly stimulated by the presence of low concentrations of a detergent, and the stimulation was much more marked with bound ribosomes than with free ribosomes. The release of nascent peptides from ribosomes could be carried out...

journal_title：Journal of biochemistry

authors： Oda T,Nabi N,Omura T

更新日期：1981-03-01 00:00:00

abstract：:ADAMTS13 is the metalloprotease responsible for the proteolytic degradation of von Willebrand factor (VWF). A severe deficiency of this VWF-cleaving protease activity causes thrombotic thrombocytopenic purpura. This protease, comprising 1,427 amino acid residues, is composed of multiple domains, i.e., a preproregion, ...

journal_title：Journal of biochemistry

authors： Soejima K,Nakamura H,Hirashima M,Morikawa W,Nozaki C,Nakagaki T

更新日期：2006-01-01 00:00:00

abstract：:When crude neurofilaments were dissolved in a solution containing 8 M urea and 1% beta-mercaptoethanol (beta-ME), the component proteins of the neurofilaments and other contaminating filaments were solubilized into monomeric forms. However, when reassembled filaments were solubilized again by the addition of urea to 8...

journal_title：Journal of biochemistry

authors： Tokutake S,Tanaka K

更新日期：1989-01-01 00:00:00

abstract：:The complete peptide map of purified recombinant human interleukin 5 (rhIL-5) was determined to verify its primary structure, glycosylation sites, and disulfide bonding structure. Each peptide fragment generated by Achromobacter protease I (API) digestion was purified and characterized by amino acid analysis and amino...

journal_title：Journal of biochemistry

authors： Minamitake Y,Kodama S,Katayama T,Adachi H,Tanaka S,Tsujimoto M

更新日期：1990-02-01 00:00:00

abstract：:An inorganic pyrophosphatase [EC 3.6.1.1] was isolated from Thiobacillus thiooxidans and purified 975-fold to a state of apparent homogeneity. The enzyme catalyzed the hydrolysis of inorganic pyrophosphate and no activity was found with a variety of other phosphate esters. The cation Mg2+ was required for maximum acti...

journal_title：Journal of biochemistry

authors： Tominaga N,Mori T

更新日期：1977-02-01 00:00:00

abstract：:1. 3'-Guanylyl-ethanol, 3'-guanylyl-propanol, and 3'-guanylyl-alpha-glycerol were synthesized by ribonuclease N1 [EC 3.1.4.8] using guanosine 2',3'-cyclic phosphate as a phosphate donor and various alcohols as phosphate acceptors. The yields of these phosphodiesters were 15%, 13.5%, 38.2%, respectively, with respect t...

journal_title：Journal of biochemistry

authors： Tamanoi F,Uchida T,Egami F,Oshima T

更新日期：1976-07-01 00:00:00

abstract：:Effects of rice bran agglutinin (RBA) on human monoblastic leukemia U937 cells were examined in comparison with those of wheat germ agglutinin (WGA) and Viscum album agglutinin (VAA). These lectins inhibit cell growth, and several lines of evidence indicate that the growth inhibition is caused by the induction of apop...

journal_title：Journal of biochemistry

authors： Miyoshi N,Koyama Y,Katsuno Y,Hayakawa S,Mita T,Ohta T,Kaji K,Isemura M

更新日期：2001-12-01 00:00:00

abstract：:Aorta smooth muscle myosin contains two kinds of 17-kDa essential light chain, LC17nm (nonmuscle-type) and LC17gi (gizzard-type) [Hasegawa, Y., Ueda, Y., Watanabe, M., & Morita, F. (1992) J. Biochem. 111, 798-803]. The LC17 isoforms were released from porcine aorta myosin by incubation at 46 degrees C. The rate of rel...

journal_title：Journal of biochemistry

authors： Hasegawa Y,Morita F

更新日期：1992-06-01 00:00:00

abstract：:Coenzyme A (CoASH) is an obligate cofactor for lipids undergoing beta-oxidation in peroxisomes. Although the peroxisomal membrane appears to be impermeable to CoASH, peroxisomes contain their own pool of CoASH. It is believed that CoASH enters peroxisomes as acyl-CoAs, but it is not known how this pool is regulated. T...

journal_title：Journal of biochemistry

authors： Reilly SJ,Tillander V,Ofman R,Alexson SE,Hunt MC

更新日期：2008-11-01 00:00:00

abstract：:The catalytic properties of a new type of dihydropteridine reductase, NADPH-specific dihydropteridine reductase [EC 1.6.99.10], from bovine liver, were studied and compared with those of the previously characterized enzyme, NADH-specific dihydropteridine reductase [EC 1.6.99.7]. With quinonoid-dihydro-6-methylpterin, ...

journal_title：Journal of biochemistry

authors： Nakanishi N,Hasegawa H,Akino M,Yamada S

更新日期：1986-03-01 00:00:00

abstract：:Paranitrophenylphosphate (pNPP) induced fluorescence changes in fluorescence isothiocyanate (FITC)-labeled Na+,K(+)-ATPase preparations. The extents of changes were similar to those induced by acetylphosphate (AcP) accompanying accumulation of a K(+)-sensitive phosphoenzyme (E2P) and an ouabain bound phosphoenzyme in ...

journal_title：Journal of biochemistry

authors： Yamazaki A,Kaya S,Tsuda T,Araki Y,Hayashi Y,Taniguchi K

更新日期：1994-12-01 00:00:00

abstract：:The conformation and stabilities of the CL fragment isolated from a type lambda Bence Jones protein and the fragment in which the intrachain disulfide bond had been reduced were studied by measuring CD, fluorescence, and ultraviolet absorption. The results indicated that no great conformational change occurs on reduct...

journal_title：Journal of biochemistry

authors： Goto Y,Hamaguchi K

更新日期：1979-11-01 00:00:00

abstract：:RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

journal_title：Journal of biochemistry

authors： Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

更新日期：2020-11-01 00:00:00

abstract：:We have cloned a chitinase cDNA (MpChi-1) from plantain (Musa paradisiacal L) using rapid amplification of cDNA ends (RACE) according to a sequence fragment which we had cloned using the suppression subtractive hybridization (SSH) technique. The MpChi-1 encodes a protein of 326 amino acids and belongs to acidic chitin...

journal_title：Journal of biochemistry

authors： Fan J,Wang H,Feng D,Liu B,Liu H,Wang J

更新日期：2007-11-01 00:00:00

abstract：:The gene coding for a Trichosanthes trypsin inhibitor analog (Ala-6-TTI) in which methionine at position 6 was replaced by alanine was synthesized chemically. The synthetic gene was cloned into plasmid pWR590-1 and expressed in Escherichia coli as a fusion protein composed of beta-galactosidase fragment of 590 amino a...

journal_title：Journal of biochemistry

authors： Chen XM,Qian YW,Chi CW,Gan KD,Zhang MF,Chen CQ

更新日期：1992-07-01 00:00:00

abstract：:Previous studies have shown that purified mitochondrial outer membrane is able to catalyze the transfer of sialic acid from CMP-Neu5Ac to an exogenous asialoglycoprotein acceptor, asialofetuin. Considering the heterogeneity of the glycan chains borne by this glycoprotein, an investigation of mitochondrial sialyltransf...

journal_title：Journal of biochemistry

authors： Gasnier F,Baubichon-Cortay H,Louisot P,Gateau-Roesch O

更新日期：1991-11-01 00:00:00

abstract：:Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

journal_title：Journal of biochemistry

authors： Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

更新日期：2001-06-01 00:00:00

abstract：:Two DFP-sensitive alkaline proteinases with strong esterase activity toward Ac-(Ala)3-OMe, designated as alkaline serine proteinases D and E, were purified pronase, a protease mixture from St. griseus K-1. Each was shown to be homogeneous by acrylamide disc gel electrophoresis. The molecular weights of these enzymes w...

journal_title：Journal of biochemistry

authors： Narahashi Y,Yoda K

更新日期：1977-03-01 00:00:00

abstract：:3-Isopropylmalate dehydrogenase (IPMDH) is a dimeric enzyme with a strongly hydrophobic core that is composed of residues from four alpha-helices. We replaced Glu253, which is found in the hydrophobic core and is part of the subunit interface of the Bacillus subtilis (Bs) IPMDH, with several other amino acids to probe...

journal_title：Journal of biochemistry

authors： Ohkuri T,Yamagishi A

更新日期：2007-06-01 00:00:00