Isolation of glycopeptides from the lectin-stimulated human peripheral lymphocyte cell surface.

abstract：:The effects of pH on the thermodynamic properties of the proton-translocating cyclic electron transfer system in a purple photosynthetic bacterium Chromatium vinosum were studied. Two thermodynamic parameters, the flux (Je) and force (deltamue) of the electron transfer process, were analyzed. The rate of electron tran...

journal_title：Journal of biochemistry

authors： Arata H,Nishimura M

更新日期：1979-02-01 00:00:00

abstract：:Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating in normal somatic cells...

journal_title：Journal of biochemistry

authors： Nozawa K,Kurumiya Y,Yamamoto A,Isobe Y,Suzuki M,Yoshida S

更新日期：1999-08-01 00:00:00

abstract：:A new multifunctional protein kinase, which normally exists as an inactive form in the soluble fraction in mammalian tissues, attaches to membranes to exhibit full enzymatic activity. A low concentration of Ca2+ is absolutely necessary for this activation. This process is reversible. cAMP shows no effect. The active f...

journal_title：Journal of biochemistry

authors： Takai Y,Kishimoto A,Iwasa Y,Kawahara Y,Mori T,Nishizuka Y,Tamura A,Fujii T

更新日期：1979-08-01 00:00:00

abstract：:We studied enzymatic activities in sea urchin egg extracts that phosphorylate myosin regulatory light chain (MRLC) from chicken gizzard smooth muscle. The activity in the presence of EGTA showed cell cycle-dependent changes similar to that of histone H1 kinase, namely, it peaked shortly before cleavage, while that in ...

journal_title：Journal of biochemistry

authors： Mishima M,Mabuchi I

更新日期：1996-05-01 00:00:00

abstract：:Many eukaryotic genes are interrupted by introns, which are removed from mRNA precursors by the RNA splicing mechanism. Although nucleotide sequences around splice sites have considerable homology among various genes, exact splice-site signals are unknown. Previously, applying the computer searching method to primary ...

journal_title：Journal of biochemistry

authors： Iida Y

更新日期：1985-04-01 00:00:00

abstract：:Acyl-CoA oxidase, the initial enzyme of the peroxisomal beta-oxidation system, was purified from rat liver. The final preparation was judged to be nearly homogeneous from the results of sedimentation analysis. Ultrogel AcA-34 column chromatography, and phosphocellulose column chromatography. The molecular weight of th...

journal_title：Journal of biochemistry

authors： Osumi T,Hashimoto T,Ui N

更新日期：1980-06-01 00:00:00

abstract：:We recently found that budding yeast Saccharomyces cerevisiae sigma1278b, but not genome project strain S288C, has a gene conferring resistance to L-azetidine-2-carboxylic acid (AZC), a toxic four-membered ring analogue of L-proline. Also, the gene, designated as MPR1, encodes a novel acetyltransferase that detoxifies...

journal_title：Journal of biochemistry

authors： Nomura M,Nakamori S,Takagi H

更新日期：2003-01-01 00:00:00

abstract：:An acid proteinase different from pepsin was purified from bullfrog (Rana catesbeiana) gastric mucosa by chromatography on hydroxyapatite, Q-Sepharose, Con A-Sepharose 4B, and Mono Q columns. Its molecular weight after purification was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 45 kDa...

journal_title：Journal of biochemistry

authors： Inokuchi T,Kobayashi K,Horiuchi S

更新日期：1994-01-01 00:00:00

abstract：:Lambda dv plasmids having an amber mutation in an initiator gene, O or P, were constructed from mutant lambda phages by recombinant DNA techniques and several properties of such derivatives were investigated. These plasmids are perpetuated in suppressor-plus (amber-permissive) cells, but not in non-suppressor cells. T...

journal_title：Journal of biochemistry

authors： Miwa T,Akaboshi E,Matsubara K

更新日期：1983-08-01 00:00:00

abstract：:To study how collagen synthesis is regulated in developing chick embryonic skin, hydroxyproline synthesis, incorporation of proline, and translational activity and content of collagen mRNA in 12-, 15-, and 18-day skins were determined and compared with each other. Hydroxyproline synthesis in the 18-day skins was marke...

journal_title：Journal of biochemistry

authors： Ohyama K,Tokimitsu I,Tajima S

更新日期：1991-08-01 00:00:00

abstract：:The effects of unsaturated fatty acids on the activities of peroxisomal enzymes of Tetrahymena pyriformis were investigated. When saturated fatty acids and the corresponding unsaturated fatty acids (C18) were added to the culture medium at 0.05%, the activities of peroxisomal enzymes [fatty acyl-CoA oxidase (FAO), car...

journal_title：Journal of biochemistry

authors： Gotoh K,Takei M,Watanabe T,Suga T

更新日期：1986-10-01 00:00:00

abstract：:Glycoengineering of therapeutic proteins has been applied to improve the clinical efficacy of several therapeutics. Here, we examined the effect of glycosylation on the properties of the Fab of the therapeutic antibody, adalimumab. An N-glycosylation site was introduced at position 178 of the H-chain constant region o...

journal_title：Journal of biochemistry

authors： Nakamura H,Kiyoshi M,Anraku M,Hashii N,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2020-10-27 00:00:00

abstract：:Sporulation of Bacillus subtilis, growing exponentially in the presence of rapidly metabolizable nutrients, was induced by addition of decoyinine (an antibiotic inhibitor of GMP synthesis), and intracellular amounts of ppGpp were determined after 2 M formic acid extraction by polyethyleneimine (PEI)-cellulose thin-lay...

journal_title：Journal of biochemistry

authors： Ikehara K,Okamoto M,Sugae K

更新日期：1982-03-01 00:00:00

abstract：:Deoxyhypusine synthase catalyzes the NAD+-dependent formation of deoxyhypusine in the eIF-5A precursor protein by transferring the 4-aminobutyl moiety of spermidine. This enzyme has recently been shown to be essential for cell viability and growth of yeast [Sasaki, K., Abid, M.R., and Miyazaki, M. (1996) FEBS Lett. 38...

journal_title：Journal of biochemistry

authors： Abid MR,Sasaki K,Titani K,Miyazaki M

更新日期：1997-04-01 00:00:00

abstract：:Aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidase activity using quinol in the respiratory chain. In the previous work, we have characterized recombinant QPO purified from t...

journal_title：Journal of biochemistry

authors： Abe T,Kawarai T,Takahashi Y,Konishi K

更新日期：2017-06-01 00:00:00

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:An ATP-dependent DNase has been purified from Thermus thermophilus HB8 by a procedure involving streptomycin precipitation, DEAE-cellulose chromatography, Sephadex G-200 gel filtration and heparin-agarose affinity chromatography. ATP-dependent DNase activity was separated into two distinct peaks, Peak A and Peak B, by...

journal_title：Journal of biochemistry

authors： Watanabe N,Umeno M,Anai M

更新日期：1983-02-01 00:00:00

abstract：:Two transferrin (Tf) glycan-isoforms were previously found in cerebrospinal fluid (CSF); one appears to be derived from serum (Tf-2) and the other from choroid plexus, a CSF-producing tissue (Tf-1). To analyse metabolic differences associated with the two isoforms, their ratio (Tf-2/Tf-1) was defined as the Tf index. ...

journal_title：Journal of biochemistry

authors： Yoshihara A,Fukatsu M,Hoshi K,Ito H,Nollet K,Yamaguchi Y,Ishii R,Tokuda T,Miyajima M,Arai H,Kato T,Furukawa K,Arai H,Kikuchi A,Takeda A,Ugawa Y,Hashimoto Y

更新日期：2016-08-01 00:00:00

abstract：:In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

journal_title：Journal of biochemistry

authors： Egami F

更新日期：1975-06-01 00:00:00

abstract：:The kinetics of alpha-crystallin reaggregation in the presence of calcium ions were investigated by monitoring changes in optical density at 320 nm. alpha-Crystallin showed a critical concentration. In the initial stages, reaggregation and deaggregation of alpha-crystallin may be essentially fully reversible in the pr...

journal_title：Journal of biochemistry

authors： Sano Y

更新日期：1980-09-01 00:00:00

abstract：:NADP+-specific isocitrate dehydrogenase was found in several tissues of the pupa of the silkworm, Bombyx mori. This enzyme was highly purified from the whole bodies of pupae. This is the first isolation of the enzyme from insect materials. The purified enzyme gave a single protein band on polyacrylamide gel electropho...

journal_title：Journal of biochemistry

authors： Miake F,Torikata T,Koga K,Hayashi K

更新日期：1977-08-01 00:00:00

abstract：:A number of L-amino acids and derivatives were tested as substrates for the purified Pseudomonas L-phenylalanine oxidase. The reaction products of these amino acids were analyzed by high performance liquid chromatography and the kinetic properties of the reactions were partially characterized. In addition to L-phenyla...

journal_title：Journal of biochemistry

authors： Koyama H

更新日期：1984-08-01 00:00:00

abstract：:1. Native human hemoglobin A was digested with proteases after treatment with reduced or oxidized glutathione, cysteine, or cystine. 2. The treatment of hemoglobin with a 40-fold molar excess of reduced glutathione or cysteine at 37 degrees C for 4 enhanced its susceptibility to trypsin 4 or 6 times, respectively. How...

journal_title：Journal of biochemistry

authors： Kimura H,Murata H,Uematsu H

更新日期：1980-08-01 00:00:00

abstract：:We designed a simple procedure for the purification of peptidylarginine deiminase, which catalyzes the deimination of arginyl residues in protein, from rabbit skeletal muscle using substrate affinity chromatography. Of the immobilized substrate ligands tested, i.e. protamine and soybean trypsin inhibitor (Kunitz) (STI...

journal_title：Journal of biochemistry

authors： Takahara H,Okamoto H,Sugawara K

更新日期：1986-05-01 00:00:00

abstract：:Incubation of quiescent cultures of Swiss 3T3 cells with epidermal growth factor (EGF) caused an increase in c-myc mRNA. Under these conditions, EGF did not induce phosphoinositide turnover, formation of diacylglycerol, formation of inositol tris-, bis-, and monophosphates, protein kinase C activation, or Ca2+ mobiliz...

journal_title：Journal of biochemistry

authors： Tsuda T,Hamamori Y,Fukumoto Y,Kaibuchi K,Takai Y

更新日期：1986-12-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:Isolation and characterization of a fibrous protein component which might be associated with contractile ring of a dividing Tetrahymena cell were attempted by making use of coprecipitation of the protein with rabbit skeletal muscle myosin. The protein was purified to homogeneity by ammonium sulfate fractionation betwe...

journal_title：Journal of biochemistry

authors： Numata O,Yasuda T,Hirabayashi T,Watanabe Y

更新日期：1980-11-01 00:00:00

abstract：:The structure of a ferricytochrome c' extracted from Rhodospirillum rubrum has been determined at 6 A resolution by the X-ray crystallographic method. The crystals, obtained by dialyzing the protein solution against polyethylene glycol 4000, belong to the hexagonal space group P6(1). Two heavy atom derivatives were ob...

journal_title：Journal of biochemistry

authors： Yasui M,Harada S,Kai Y,Kasai N

更新日期：1985-07-01 00:00:00

abstract：:The interaction between n-octyl-beta-D-glucopyranoside (octyl glucoside) and bovine liver glutamate dehydrogenase (GDH) was studied using techniques including equilibrium dialysis, UV-spectrophotometry, circular dichroism (CD), fluorescence energy transfer and extrinsic spectrofluorometry in 50 mM sodium phosphate buf...

journal_title：Journal of biochemistry

authors： Ghobadi S,Safarian S,Moosavi-Movahedi AA,Ranjbar B

更新日期：2001-11-01 00:00:00

abstract：:The FLI-1 transcription factor is a member of the ETS gene family, most closely related to ERG. In this study, the FLI-1 protein products were characterized using a specific monoclonal antibody previously developed against bacterially expressed protein. In the human T-cell line Jurkat, both isoforms of FLI-1, p51 and ...

journal_title：Journal of biochemistry

authors： Zhang XK,Watson DK

更新日期：2005-03-01 00:00:00