Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes.

abstract：:Synthesis and degradation of glycerophospholipids in HL-60 cells and retinoic acid (RA)-treated HL-60 cells were examined. The synthesis of each subclass of ethanolamine-containing glycerophospholipids was extremely suppressed in RA-treated HL-60 cells, while that of other glycerophospholipids was not seriously affect...

journal_title：Journal of biochemistry

authors： Naito M,Kudo I,Nakagawa Y,Waku K,Nojiri H,Saito M,Inoue K

更新日期：1991-10-01 00:00:00

abstract：:1. When ribonuclease T1 [EC 3.1.4.8] (0.125% solution) was treated with a 760-fold molar excess of iodoacetamide at pH 8.0 and 37 degrees, about 90% of the original activity was lost in 24 hr. The half-life of the activity was about 8 hr. The binding ability for 3'-GMP was lost simultaneously. Changes were detected on...

journal_title：Journal of biochemistry

authors： Takahashi K

更新日期：1976-12-01 00:00:00

abstract：:Maitotoxion, a putative Ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate Gambierdiscus toxicus as a colorless amorphous solid. The toxin reacted positively to Dragendorff's reagent but not to ninhydrin reagent. The mouse lethality of maitotoxin determined by intraperitoneal injection was 0...

journal_title：Journal of biochemistry

authors： Yokoyama A,Murata M,Oshima Y,Iwashita T,Yasumoto T

更新日期：1988-08-01 00:00:00

abstract：:A novel positively charged planar bilayer membrane was formed from a mixture containing 20% cationic lipid, 1,2-dioleoyloxy-3-(trimethylammonio)propane, and neutral phospholipid mixture of 56% phosphatidylethanolamine and 24% egg phosphatidylcholine. The basic properties of the bilayer were essentially the same as tho...

journal_title：Journal of biochemistry

authors： Anzai K,Masumi M,Kawasaki K,Kirino Y

更新日期：1993-10-01 00:00:00

abstract：:The immunological properties of gamma-glutamyltransferases (gamma-GTs) from human serum, liver and tonsil were studied by using a monospecific antibody to human kidney gamma-GT for the purpose of elucidating their isozymic relationships. gamma-GTs partially purified from liver and tonsil were indistinguishable in this...

journal_title：Journal of biochemistry

authors： Tsuchida S,Imai F,Sato K

更新日期：1981-03-01 00:00:00

abstract：:Methylobacterium extorquens AM1 is an aerobic facultative methylotroph known to secrete pyrroloquinoline quinone (PQQ), a cofactor of a number of bacterial dehydrogenases, into the culture medium. To elucidate the molecular mechanism of PQQ biosynthesis, we are focusing on PqqE which is believed to be the enzyme catal...

journal_title：Journal of biochemistry

authors： Saichana N,Tanizawa K,Pechoušek J,Novák P,Yakushi T,Toyama H,Frébortová J

更新日期：2016-01-01 00:00:00

abstract：:In dystrophic hamsters, increases in the levels of cathepsin B plus L and thiol proteinase inhibitor were marked in skeletal muscle, but only slight in heart muscle. The lysosomal hydrolases did not increase in parallel in dystrophic muscle: cathepsin B plus L and beta-glucuronidase increased, but cathepsin C and acid...

journal_title：Journal of biochemistry

authors： Kominami E,Bando Y,Ii K,Hizawa K,Katunuma N

更新日期：1984-12-01 00:00:00

abstract：:Neutral alpha-glucosidase was partially purified from granular fractions isolated from guinea pig polymorphonuclear leukocytes (PMNL). The native enzyme had a high molecular weight, about 417,000, with a subunit of 43,000. The purified enzyme hydrolysed 4-methylumbelliferyl alpha-glucoside and maltose, but not isomalt...

journal_title：Journal of biochemistry

authors： Imai K,Harada T,Takano Y,Morikawa S,Tanaka A

更新日期：1989-10-01 00:00:00

abstract：:Subtilisin BPN' was chemically converted to thiolsubtilisin and the interaction of this modified enzyme with Streptomyces subtilisin inhibitor (SSI) was examined. SSI competitively inhibited the esterolytic activity of thiolsubtilisin toward p-nitrophenyl acetate with a K1 value of 1.3 X 10(-5) M at pH 7.5 Spectrophot...

journal_title：Journal of biochemistry

authors： Fujiwara K,Inouye K,Tonomura B,Murao S,Tsuru D

更新日期：1977-07-01 00:00:00

abstract：:The three-dimensional structure of rat-liver acyl-CoA oxidase-II (ACO-II) in a complex with a C12-fatty acid was solved by the molecular replacement method based on the uncomplexed ACO-II structure. The crystalline form of the complex was obtained by cocrystallization of ACO-II with dodecanoyl-CoA. The crystalline com...

journal_title：Journal of biochemistry

authors： Tokuoka K,Nakajima Y,Hirotsu K,Miyahara I,Nishina Y,Shiga K,Tamaoki H,Setoyama C,Tojo H,Miura R

更新日期：2006-04-01 00:00:00

abstract：:Caldesmon, calmodulin-, and actin-binding protein of chicken gizzard did not affect the process of polymerization of actin induced by 0.1 M KCl. Caldesmon binds to F-actin, thus inhibiting the gelation action of actin binding protein (ABP; filamin). Low shear viscosity and flow birefringence measurements revealed that...

journal_title：Journal of biochemistry

authors： Maruyama K,Morimoto K,Kijima Y,Sobue K,Kakiuchi S

更新日期：1985-05-01 00:00:00

abstract：:Diacylglycerol acyltransferase (DGAT) was examined as a key enzyme for triacylglycerol (TG) accumulation of an oleaginous fungus, Mortierella ramanniana var. angulispora. Subcellular fractionation of the fungus showed that DGAT activity was highest in the lipid body fraction, which occupied 77% of the recovered DGAT a...

journal_title：Journal of biochemistry

authors： Kamisaka Y,Nakahara T

更新日期：1994-12-01 00:00:00

abstract：:The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1, 616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells tran...

journal_title：Journal of biochemistry

authors： Kim DH,Inagaki Y,Suzuki T,Ioka RX,Yoshioka SZ,Magoori K,Kang MJ,Cho Y,Nakano AZ,Liu Q,Fujino T,Suzuki H,Sasano H,Yamamoto TT

更新日期：1998-12-01 00:00:00

abstract：:Eukaryotic typical 2-Cys type peroxiredoxin (Prx) is inactivated by hyperoxidation of the peroxidatic cysteine to a sulphinic acid in a catalytic cycle-dependent manner. This inactivation process has been well documented for cytosolic isoforms of Prx. However, such a hyperoxidative inactivation has not fully been inve...

journal_title：Journal of biochemistry

authors： Ikeda Y,Nakano M,Ihara H,Ito R,Taniguchi N,Fujii J

更新日期：2011-04-01 00:00:00

abstract：:An enzyme which catalyzes the hydrolysis of acetamido groups of N-acetylglucosamine residues in cell wall peptidoglycan was found in the supernatant and 20,000 X g pellet fractions of Bacillus cereus. Autolysis of the latter fraction resulted in solubilization and activation of the deacetylase. Among various bacteria,...

journal_title：Journal of biochemistry

authors： Araki Y,Oba S,Araki S,Ito E

更新日期：1980-08-01 00:00:00

abstract：:To investigate the role of carboxyl groups of glucoamylases [EC 3.2.1.3] from a Rhizopus sp. (Gluc1 and Gluc2), the modification of Gluc1 and Gluc2 with a water-soluble carbodiimide, 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate (CMC), was studied. The inactivation of Gluc1 proceeded wi...

journal_title：Journal of biochemistry

authors： Iwama M,Ohtsuki R,Takahashi T,Irie M

更新日期：1984-08-01 00:00:00

abstract：:1. The endogenous phosphorylation of mouse brain microsomes was studied using the technique of acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). 2. It was found that specific proteins and lipids in brain microsomes were phosphorylated by the terminal phosphate of ATP under appropriate con...

journal_title：Journal of biochemistry

authors： Satomi D

更新日期：1977-12-01 00:00:00

abstract：:We expressed human myotonic dystrophy protein kinase (DMPK) in the fission yeast Schizosaccharomyces pombe, in which the overexpression of human DMPK affects cell growth and cell shape. The human DMPK protein has a leucine-rich domain at the N-terminus, a serine/threonine kinase domain in the middle, and a hydrophobic...

journal_title：Journal of biochemistry

authors： Sasagawa N,Kino Y,Takeshita Y,Oma Y,Ishiura S

更新日期：2003-10-01 00:00:00

abstract：:Frontal gel chromatography is a convenient and accurate method to obtain the free ligand concentration of a protein-ligand mixture. Because a large amount of sample (more than 6 ml) is required for the method, it has been rarely used for binding experiments. We have developed a system to carry out frontal gel chromato...

journal_title：Journal of biochemistry

authors： Honjo M,Ishida T,Horiike K

更新日期：1997-08-01 00:00:00

abstract：:The crystal structure of an adenylate kinase from an extremophilic archaeon Aeropyrum pernix was determined in complex with full ligands, ATP-Mg2+ and AMP, at a resolution of 2.0 Å. The protein forms a trimer as found for other adenylate kinases from archaea. Interestingly, the reacting three atoms, two phosphorus and...

journal_title：Journal of biochemistry

authors： Shibanuma Y,Nemoto N,Yamamoto N,Sampei GI,Kawai G

更新日期：2020-09-01 00:00:00

abstract：:Beta-N-Acetylgalactosaminidase [EC 3.2.1.53] was purified to homogeneity from the culture media of Bacillus sp. AT173-1. The enzyme has a molecular weight of 48,000 as estimated by SDS-PAGE under reducing conditions and an isoelectric point of 4.3. The enzyme requires dithiothreitol as an activator and is most active ...

journal_title：Journal of biochemistry

authors： Tanaka A,Ozaki S

更新日期：1997-08-01 00:00:00

abstract：:The 20,000 dalton light chain (L2) was isolated from rabbit and chicken striated muscle myosins, and the Ca2+-induced conformational changes of these proteins were investigated. 1) The reaction of thiol groups of L2 with dithiobisnitrobenzoic acid (DTNB), 2) measurements of the UV difference absorption spectrum, 3) me...

journal_title：Journal of biochemistry

authors： Minowa O,Matsuda S,Yagi K

更新日期：1983-07-01 00:00:00

abstract：:The voltage-dependent L-type Ca2+ channel in the heart is regulated by cAMP-dependent protein kinase (PKA) and possibly by protein kinase C (PKC). We have investigated the channel modulation through phosphorylation by these protein kinases, using liposomes into which Ca2+ channels from bovine heart were reconstituted....

journal_title：Journal of biochemistry

authors： Kameyama A,Shearman MS,Sekiguchi K,Kameyama M

更新日期：1996-07-01 00:00:00

abstract：:We investigated the significance of the plasma membrane lipid composition in exocytosis in an in vitro interaction system using an intact secretory granular fraction (SG) isolated from the rat parotid gland. When various liposomes (as a model of plasma membranes) were added to this assay system, rapid and transient am...

journal_title：Journal of biochemistry

authors： Mizuno-Kamiya M,Inokuchi H,Kameyama Y,Yashiro K,Shin SO,Fujita A

更新日期：1995-10-01 00:00:00

abstract：:Six major acidic glycosphingolipids were isolated from human amnion using DEAE Sephadex A-25 and silica beads column chromatography. The structures of these glycosphingolipids were determined by methylation analysis, TLC immunostaining and/or negative ion FAB-MS, and were concluded to be II3 alpha NeuAcLacCer(GM3), IV...

journal_title：Journal of biochemistry

authors： Asano K,Suzuki M,Handa S,Oshima M

更新日期：1991-01-01 00:00:00

abstract：:Raman spectroscopy was used to investigate the hydrogen bonding at the C(4)=O moiety of the isoalloxazine nucleus in a series of flavins and flavoproteins. Isotope effects of Raman bands confirmed that the band observed around 1,710 cm(-1) is mainly derived from C(4)=O stretching vibrational mode. A linear correlation...

journal_title：Journal of biochemistry

authors： Hazekawa I,Nishina Y,Sato K,Shichiri M,Miura R,Shiga K

更新日期：1997-06-01 00:00:00

abstract：:After a dose of 10 micrograms of 6-aminonicotinamide (6-AN) was administered to day-4- chick embryo in ovo, micromelia was obviously observed in the hind limbs of 7-day chick embryos. We examined the teratogenic mechanism of 6-AN by using the normal or micromelial hind limbs (buds) from day 5 to day 7, with special at...

journal_title：Journal of biochemistry

authors： Honda A,Iwama M,Umeda T,Mori Y

更新日期：1982-06-01 00:00:00

abstract：:Anti-cobrotoxin antibodies can be separated into precipitin and non-precipitin antibodies. The precipitin antibody possesses the same binding affinity to cobrotoxin as non-precipitin antibody, but the neutralizing capability of the latter is superior to that of the former in blocking cobrotoxin binding to nicotinic ac...

journal_title：Journal of biochemistry

authors： Kuo KW,Chang LS,Lin PM,Leaber RJ,Chang CC

更新日期：1994-12-01 00:00:00

abstract：:We cloned and sequenced the Serratia marcescens prolyl aminopeptidase (SPAP) gene. Nucleotide sequence analysis revealed an open reading frame of 951 bp, encoding a protein of 317 amino acids with a predicted molecular weight of 36,083. The expressed enzyme was purified about 90-fold on columns of Toyopearl HW65C and ...

journal_title：Journal of biochemistry

authors： Kabashima T,Kitazono A,Kitano A,Ito K,Yoshimoto T

更新日期：1997-09-01 00:00:00

abstract：:The mammalian ATP/ADP translocator isoform-2 (ANT2) gene is growth-activated. Regulation of the gene appears to involve Sp1, as an essential activator, and a suppressor through an Sp1 core element next to the transcription start. We show here that the proximal promoter also binds AP-2 strongly and specifically to two ...

journal_title：Journal of biochemistry

authors： Li R,Hodny Z,Zaid A,Barath P,Nelson BD

更新日期：1999-07-01 00:00:00