Lecithin: cholesterol acyltransferase is insufficient to prevent oxidative modification of low-density lipoprotein.

abstract：:1. When ribonuclease T1 [EC 3.1.4.8] (0.125% solution) was treated with a 760-fold molar excess of iodoacetamide at pH 8.0 and 37 degrees, about 90% of the original activity was lost in 24 hr. The half-life of the activity was about 8 hr. The binding ability for 3'-GMP was lost simultaneously. Changes were detected on...

journal_title：Journal of biochemistry

authors： Takahashi K

更新日期：1976-12-01 00:00:00

abstract：:The role of thiols on the activation and/or stabilization of rat brain nitric oxide synthase (NOS) has been investigated. It was found that thiols are not necessary for stabilizing or protecting the protein during purification but are required during enzyme turnover for maximum activity. In the complete absence of thi...

journal_title：Journal of biochemistry

authors： Komori Y,Hyun J,Chiang K,Fukuto JM

更新日期：1995-04-01 00:00:00

abstract：:Micromolar levels of free calcium ions added to the extracellular medium elicit secretion of serotonin from electropermeabilized bovine platelets in the presence of millimolar levels of Mg-ATP. Such Ca2(+)-dependent secretion of serotonin was almost completely impaired when the permeabilized platelets were preincubate...

journal_title：Journal of biochemistry

authors： Morimoto T,Oho C,Ueda M,Ogihara S,Takisawa H

更新日期：1990-08-01 00:00:00

abstract：:The structure and expression of the pentraxins, serum amyloid P component (SAP), and C-reactive protein (CRP), have been investigated in the guinea pig. Northern blot analysis of hepatic RNA from animals in which acute inflammation had been induced by intraperitoneal injection of thioglycollate established that neithe...

journal_title：Journal of biochemistry

authors： Rubio N,Sharp PM,Rits M,Zahedi K,Whitehead AS

更新日期：1993-03-01 00:00:00

abstract：:Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry 20, 2945-2950) that these fragments can be separated by gel filtration i...

journal_title：Journal of biochemistry

authors： Hozumi T

更新日期：1981-09-01 00:00:00

abstract：:After screening 900 E. coli strains of the Clarke and Carbon collection for by lysophospholipase L1 activities, we isolated a clone bearing the plasmid pLC6-34, which showed an increased level of lysophospholipase L1 activity. Strains bearing the plasmid pC124, a subclone of pLC6-34 in plasmid vector pUC8, showed appr...

journal_title：Journal of biochemistry

authors： Karasawa K,Kudo I,Kobayashi T,Homma H,Chiba N,Mizushima H,Inoue K,Nojima S

更新日期：1991-02-01 00:00:00

abstract：:We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted into liposomes comprising Salmonella minnesota R595 lipopolysaccharides, and fusion of spleen cells with a mouse myeloma cell...

journal_title：Journal of biochemistry

authors： Watarai S,Kushi Y,Shigeto R,Misawa N,Eishi Y,Handa S,Yasuda T

更新日期：1995-05-01 00:00:00

abstract：:The complement system is a classic central player in innate immunity. Most pathogens activate both complement and the toll-like receptor (TLR) pathway. Therefore, to provide a more comprehensive understanding of innate immunity, it is important to understand the crosstalk between these two systems. Mouse macrophages p...

journal_title：Journal of biochemistry

authors： Okazaki N,Hazeki K,Izumi T,Nigorikawa K,Hazeki O

更新日期：2011-03-01 00:00:00

abstract：:When an aqueous solution of a spin-labeled "two tail" gangliosidoid was incubated with liposomes or sheep erythrocytes, the broad single resonance line in the ESR spectrum disappeared and a signal showing an anisotropic motion appeared, indicating that the spin-labeled "two tail" gangliosidoid in the micellar state wa...

journal_title：Journal of biochemistry

authors： Kanda S,Inoue K,Nojima S,Utsumi H,Wiegandt H

更新日期：1982-06-01 00:00:00

abstract：:Human serum was shown to contain N-acetyllactosamine: N-acetylglucosaminyltransferase activity. The reaction product was hydrolyzed by beta-N-acetylglucosaminidase and released [14C]N-acetylglucosamine, indicating that the N-acetylglucosaminyl residue was beta-linked to N-acetyllactosamine. Methylation and hydrolysis ...

journal_title：Journal of biochemistry

authors： Hosomi O,Takeya A,Kogure T

更新日期：1984-06-01 00:00:00

abstract：:Reverse transcriptases (RTs) from Moloney murine leukaemia virus (MMLV) and avian myeloblastosis virus (AMV) contain all the fingers, palm, thumb, connection and RNase H domains. The fingers, palm and thumb domains are thought to be involved in the reverse transcription activity, and the RNase H domain is in the RNase...

journal_title：Journal of biochemistry

authors： Yasukawa K,Mizuno M,Inouye K

更新日期：2009-03-01 00:00:00

abstract：:A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and r...

journal_title：Journal of biochemistry

authors： Tsugita A,Uchida T,Mewes HW,Ataka T

更新日期：1987-12-01 00:00:00

abstract：:The subcellular distribution of calmodulin and particulate calmodulin-binding activity was studied in a eukaryotic protozoan, Tetrahymena pyriformis NT-1. The particulate calmodulin-binding activity was found to be localized principally in microsomes and to some extent in cilia and surface membranes called pellicles. ...

journal_title：Journal of biochemistry

authors： Nagao S,Banno Y,Nozawa Y,Sobue K,Yamazaki R,Kakiuchi S

更新日期：1981-09-01 00:00:00

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:A cytochrome o-type oxidase from the thermophilic bacterium PS3 grown under air-limited conditions was purified by ion-exchange chromatography in the presence of a non-ionic detergent. The enzyme was composed of three subunits (60, 30, and 16 kDa) and seemed to contain two molecules of heme b as prosthetic groups. It ...

journal_title：Journal of biochemistry

authors： Sone N,Kutoh E,Sato K

更新日期：1990-04-01 00:00:00

abstract：:Calmodulin (CaM), a physiologically important Ca(2+)-binding protein, participates in numerous cellular regulatory processes. It is dumbbell shaped and contains two globular domains connected by a short alpha-helix. Each of the globular domains has two Ca(2+)-binding sites, the EF hands. CaM undergoes a conformational...

journal_title：Journal of biochemistry

authors： Shishido H,Yamada MD,Kondo K,Maruta S

更新日期：2009-10-01 00:00:00

abstract：:The pH-activity curve of heavy meromyosin ATPase [EC 3.6.1.3] was measured at various temperatures. The pH-activity curve at higher temperatures showed a maximum at low pH and a minimum at pH 7 to 8 as has been already reported. At lower temperatures it was sigmoidal in shape, similar to a simple dissociation curve of...

journal_title：Journal of biochemistry

authors： Ishigami F,Morita F

更新日期：1977-02-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the expression of phospholipid biosynthetic genes, including the INO1 gene (encoding inositol-1-phosphate synthase), is coordinately regulated by a cis-acting transcriptional element, UAS(INO) (inositol-sensitive upstream activating sequence). For this paper we studied the effect...

journal_title：Journal of biochemistry

authors： Kagiwada S,Zen R

更新日期：2003-04-01 00:00:00

abstract：:The rates of synthesis and degradation of arginosuccinate synthetase in rat liver under various dietary conditions were determined. The relative rate of the enzyme synthesis in the livers of rats fed on 70% casein diet was 4.0 times greater than that for rats fed on 5% casein diet. The rate constants of degradation (K...

journal_title：Journal of biochemistry

authors： Tsuda M,Shikata Y,Katsunuma T

更新日期：1979-03-01 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (InsP3) releases Ca2+ from the non-mitochondrial Ca2+ store site of various types of cells. To study the mechanisms of the Ca2+ release from the store site, the effect of InsP3 on the passive Ca2+ release and influx, and the active Ca2+ uptake in the presence of oxalate, was examined using...

journal_title：Journal of biochemistry

authors： Hirata M,Kukita M,Sasaguri T,Suematsu E,Hashimoto T,Koga T

更新日期：1985-06-01 00:00:00

abstract：:The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth...

journal_title：Journal of biochemistry

authors： Ishihama A,Enami M,Nishijima Y,Fukui T,Ohtsuka E,Ikehara M

更新日期：1980-03-01 00:00:00

abstract：:Selected biochemical properties, including the charge heterodispersity profile and carbohydrate specificity, of bovine galectin-1 were determined in detail. The lectin was purified through an improved purification protocol that yielded 35-40 mg/kg of wet tissue with a specific activity of 1.7-2 x 10(4) mg-1.ml. The ga...

journal_title：Journal of biochemistry

authors： Ahmed H,Fink NE,Pohl J,Vasta GR

更新日期：1996-11-01 00:00:00

abstract：:The chromatin fraction of rat liver exhibited proteolytic activity caused by serine proteases, with maximal activity at pH 8 or 10. They were analyzed by affinity labeling with [3H]diisopropylfluorophosphate followed by SDS-polyacrylamide gel electrophoresis, and partially purified by gel filtration through Sepharose ...

journal_title：Journal of biochemistry

authors： Tsurugi K,Ogata K

更新日期：1982-11-01 00:00:00

abstract：:Induction of the enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in a medium containing straight chain saturated fatty acids was studied. The acyl-CoA dehydrogenase (ACDH) activity was induced during the exponential phase in cells grown in palmitic acid-supplemented medium, reache...

journal_title：Journal of biochemistry

authors： Sato S,Imamura S,Ozeki Y,Kawaguchi A

更新日期：1992-01-01 00:00:00

abstract：:Carboxyl groups of native human fibrinogen were modified with glycine methyl ester and 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. It seemed likely that the modification occurred stepwise. Approximately 26% of the carboxyl groups of fibrinogen was modified finally. The modified fibrinogen had no interaction with cat...

journal_title：Journal of biochemistry

authors： Kurioka S,Inoue F,Matsuda M

更新日期：1975-11-01 00:00:00

abstract：:The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic a...

journal_title：Journal of biochemistry

authors： Sato E,Uezato T,Fujita M,Nishimura K

更新日期：1982-06-01 00:00:00

abstract：:In order to determine the mode of action of cytostatic 9-beta-D-xylofuranosyladenine (xylo-A), the inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate (xylo-ATP) on DNA-dependent RNA polymerases I and II purified from cherry salmon (Oncorhynchus masou) liver nuclei were studied. This nucleotide showed ...

journal_title：Journal of biochemistry

authors： Nakayama C,Saneyoshi M

更新日期：1985-05-01 00:00:00

abstract：:Several maleimide derivatives of potential usefulness as conformational probes were tested for reactivity toward SH groups of Ca2+, Mg2+-ATPase of sarcoplasmic reticulum. These include three fluorescent labels, N-(1-anilinonaphthyl-4)maleimide (ANM), N-(p-(2-benzimidazolyl)phenyl)maleimide (BIPM), and N-(7-dimethylami...

journal_title：Journal of biochemistry

authors： Yasuoka-Yabe K,Kawakita M

更新日期：1983-09-01 00:00:00

abstract：:The physicochemical nature of an active fragment (T2A) of colicin E3 was examined together with those of its specific inhibitor, B, and the T2A-B complex under various conditions by means of CD and fluorescence spectroscopy. 1. T2A was mainly composed of unordered structure, although the molecule was folded into a rat...

journal_title：Journal of biochemistry

authors： Suzuki K,Ohno S,Imahori K

更新日期：1980-03-01 00:00:00

abstract：:Fibronectin was isolated from human placenta tissues and compared with human plasma fibronectin. Placenta and plasma fibronectins had similar amino acid compositions, immunological properties, and cell attachment-promoting activities, but differed in apparent molecular weight on sodium dodecyl sulfate-polyacrylamide g...

journal_title：Journal of biochemistry

authors： Isemura M,Yamaguchi Y,Munakata H,Aikawa J,Kan M,Yamane I,Yosizawa Z

更新日期：1984-07-01 00:00:00