Abstract:
:A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158 degrees C for reaction times of 22.5 and 45 min with 7 M HCl and 10% TFA containing 0.1% phenol.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Tsugita A,Uchida T,Mewes HW,Ataka Tdoi
10.1093/oxfordjournals.jbchem.a122209subject
Has Abstractpub_date
1987-12-01 00:00:00pages
1593-7issue
6eissn
0021-924Xissn
1756-2651journal_volume
102pub_type
杂志文章abstract::The Serratia marcescens serine protease gene encoding a 1,045-amino-acid precursor protein of 112 kDa directs excretion of the mature protease of ca. 58 kDa through the outer membrane of Escherichia coli. A typical signal peptide of 27 amino acids and a large COOH-terminal domain of the precursor are both functionally...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123809
更新日期:1992-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
doi:10.1093/jb/mvp196
更新日期:2010-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvp043
更新日期:2009-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122796
更新日期:1989-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a002818
更新日期:2001-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123937
更新日期:1992-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1976-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1999-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021730
更新日期:1997-07-01 00:00:00
abstract::Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 45...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1979-07-01 00:00:00
abstract::The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis usi...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021237
更新日期:1996-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132190
更新日期:1978-10-01 00:00:00
abstract::The modes of actions of 1-farnesylpyridinium (FPy) on yeast cell growth were investigated on the basis of its effects on cell cycle progression, morphogenesis and the related events for construction of cell wall architecture in Schizosacchromyces pombe. FPy predominantly inhibited the growth of the yeast cells after v...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvj218
更新日期:2006-12-01 00:00:00
abstract::We report here the molecular cloning and characterization of the Drosophila neutral ceramidase (CDase). Using the BLAST program, a neutral CDase homologue (AE003774) was found in the Drosophila GenBank and cloned from a cDNA library of Drosophila imaginal discs. The open reading frame of 2,112 nucleotides encoded a po...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003215
更新日期:2002-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021247
更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132079
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132703
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134806
更新日期:1984-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123102
更新日期:1990-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121769
更新日期:1986-09-01 00:00:00
abstract::The position in the acyl phosphate linkage of the phosphorylated intermediate of (Na+, K+)-ATPase that is cleaved by N-methylhydroxylamine was compared with that of the model compound acetylphosphate. The products of the cleavage of the phosphoenzyme by methylhydroxylamine were the active enzyme and a N-P compound, no...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021805
更新日期:1997-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132010
更新日期:1978-04-01 00:00:00
abstract::Candida albicans is a commensal organism, but causes life-threatening infections in immunocompromised patients. Certain factors such as yeast-hyphae transition and hydrolytic enzymes are suggested as virulence attributes of C. albicans. Among them, 10 types of secreted aspartic protease (SAP) genes have received parti...
journal_title:Journal of biochemistry
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更新日期:2011-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-02-01 00:00:00
abstract::Four distinctive DNA polymerases have been isolated from mature oocytes of the starfish, Asterina pectinifera, and characterized. The N-ethylmaleimide-resistant DNA polymerase activity was separated from the other three DNA polymerases by hydroxyapatite column chromatography. The enzyme, whose approximate sedimentatio...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/93.3.687
更新日期:1983-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133401
更新日期:1981-06-01 00:00:00
abstract::Diacylglycerol acyltransferase (DGAT) was examined as a key enzyme for triacylglycerol (TG) accumulation of an oleaginous fungus, Mortierella ramanniana var. angulispora. Subcellular fractionation of the fungus showed that DGAT activity was highest in the lipid body fraction, which occupied 77% of the recovered DGAT a...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00