The primary structures of two types of the Ulex europeus seed lectin.

abstract：:Site-directed mutagenesis studies of recombinant Trimeresurus flavoviridis venom gland phospholipase A2 (PLA2) showed that the Asn residue at position 67 takes part in recognition of the substrate 2-arachidonoyl sn-glycero-3-phosphocholine in both monomeric and micellar states. The amount of arachidonate released from...

journal_title：Journal of biochemistry

authors： Ogawa T,Shimohigashi Y,Ohno M

更新日期：1997-11-01 00:00:00

abstract：:Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...

journal_title：Journal of biochemistry

authors： Nakanishi N,Takeuchi F,Tsubaki M

更新日期：2007-11-01 00:00:00

abstract：:The three-dimensional structure of cytochrome c-553 isolated from sulfate-reducing bacterium, Desulfovibrio vulgaris Miyazaki F strain, has been determined by the multi-wavelength anomalous dispersion technique with use of synchrotron radiation. The result shows that bacterial S-class cytochromes c have a variety of f...

journal_title：Journal of biochemistry

authors： Nakagawa A,Higuchi Y,Yasuoka N,Katsube Y,Yagi T

更新日期：1990-11-01 00:00:00

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:Three forms of cytochrome P-450 of liver microsomes of 3-methylcholanthrene-treated Golden hamsters were purified and characterized as regards their catalytic activity toward aflatoxin B1-related hepatocarcinogenic mycotoxins. These include two major forms, designated as cytochrome P-450-AFB (P-450-I) and P-450-II, an...

journal_title：Journal of biochemistry

authors： Fukuhara M,Nohmi T,Mizokami K,Sunouchi M,Ishidate M Jr,Takanaka A

更新日期：1989-08-01 00:00:00

abstract：:The nucleotide sequence of the aspartate aminotransferase [EC 2.6.1.1] structural gene, aspC, of Escherichia coli K-12 was determined. The coding region of the aspC gene contained 1,188 nucleotide residues and encoded 396 amino acid residues. The amino acid sequence deduced from the nucleotide sequence agreed perfectl...

journal_title：Journal of biochemistry

authors： Kuramitsu S,Okuno S,Ogawa T,Ogawa H,Kagamiyama H

更新日期：1985-04-01 00:00:00

abstract：:The activity of biological molecules is often affected by their phosphorylation state. Regulatory phosphorylation operates as a binary switch and is usually controlled by counteracting kinases and phosphatases. However, phosphatidylinositol (PtdIns) has three phosphorylation sites on its inositol ring. The phosphoryla...

journal_title：Journal of biochemistry

authors： Miyazawa K

更新日期：2013-01-01 00:00:00

abstract：:Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...

journal_title：Journal of biochemistry

authors： Okamoto Y,Ogawa K,Motohiro T,Nishi N,Muta E,Ota S

更新日期：1993-06-01 00:00:00

abstract：:Thermal denaturation of cytochromes c of horse, cow, and Candida krusei in aqueous guanidine hydrochloride in the neutral pH region was studied by means of absorption and optical rotation measurements. The values of standard free energy change upon denaturation were estimated over the temperature range from 3 to 51 de...

journal_title：Journal of biochemistry

authors： Kawaguchi H,Noda H

更新日期：1977-05-01 00:00:00

abstract：:In a previous report (J. Biochem. 89, 1333-1335, 1981) we showed that 30 degrees C-treatment (pCa less than 6 and 2 mM MgCl2), like EDTA-treatment, caused a reversible removal of regulatory light-chains from Akazara adductor myosin. Utilizing the heat-treatment, we now show (a) that not half but the total removal of r...

journal_title：Journal of biochemistry

authors： Ojima T,Nishita K,Watanabe S

更新日期：1983-02-01 00:00:00

abstract：:The nucleotide sequence of 5S rRNA from the fission yeast, S. pombe, has been established by post labeling procedures combined with cataloging RNase T1- and A-oligonucleotides derived from unlabeled 5S rRNA. The sequence consists of 119 nucleotides without a modified base and shows more dissimilarities (at 38 position...

journal_title：Journal of biochemistry

authors： Komiya H,Miyazaki M,Takemura S

更新日期：1981-05-01 00:00:00

abstract：:The genomic organization of the gene encoding the human adipocyte-derived leucine aminopeptidase (A-LAP) has been determined. The gene is composed of 20 exons and 19 introns and spans approximately 47 kilobases of chromosome 5q15. The gluzincin aminopeptidase motif, the HEXXH(X)(18)E zinc-binding motif essential for e...

journal_title：Journal of biochemistry

authors： Hattori A,Matsumoto K,Mizutani S,Tsujimoto M

更新日期：2001-08-01 00:00:00

abstract：:The effects of freezing on Alcaligenes sp. nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, EC 1.7.2.1] dissolved in sodium phosphate (pH 7.2) were investigated. The nitrite reductase was gradually activated with time in the frozen state, resulting in an increase in its activity of 2.5-4.5 times. The...

journal_title：Journal of biochemistry

authors： Masuko M,Iwasaki H,Sakurai T,Suzuki S,Nakahara A

更新日期：1985-11-01 00:00:00

abstract：:Gp91(phox) is a key component of the phagocyte NADPH oxidase. Mutations of its promoter found in patients with chronic granulomatous disease cause deficient binding of PU.1 and HAF-1. Because the two factors bind to the same site (Pu box) of the promoter, we attempted to clarify their relative in vivo contributions to...

journal_title：Journal of biochemistry

authors： Islam MR,Fan C,Fujii Y,Hao LJ,Suzuki S,Kumatori A,Yang D,Rusvai E,Suzuki N,Kikuchi H,Nakamura M

更新日期：2002-04-01 00:00:00

abstract：:The nucleotide sequence of gene PBII encoding salivary proline-rich protein P-B was determined. PBII is 7.1 kb long and contains 3 exons. PBII exhibits considerable nucleotide sequence homology not only in exons but also in introns with PBI (accession number D89501), the gene whose nucleotide sequence was determined p...

journal_title：Journal of biochemistry

authors： Isemura S

更新日期：2000-03-01 00:00:00

abstract：:Undecaprenyl diphosphate synthase catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall. This cis-type prenyltra...

journal_title：Journal of biochemistry

authors： Kharel Y,Zhang YW,Fujihashi M,Miki K,Koyama T

更新日期：2003-12-01 00:00:00

abstract：:The entire mitochondrial genome of Rana catesbeiana was cloned into a plasmid vector pBR322 at the unique BamHI site and the nucleotide sequences of the ND2 gene and of its flanking genes were determined. The ND2 gene was encoded by 1,033 base pairs and, as deduced from the nucleotide sequence, the ND2 product consist...

journal_title：Journal of biochemistry

authors： Fujii H,Shimada T,Goto Y,Okazaki T

更新日期：1988-03-01 00:00:00

abstract：:Plasmapheresis with a dextran sulfate column is a treatment for patients with hypercholesteremia. When proteins bound to the column during the treatment were fractionated to prepare some known proteins, we found a 57 kDa glycoprotein designated GP57 which showed a new N-terminal amino acid sequence. Western-blot analy...

journal_title：Journal of biochemistry

authors： Choi-Miura NH,Sano Y,Oda E,Nakano Y,Tobe T,Yanagishita T,Taniyama M,Katagiri T,Tomita M

更新日期：1995-02-01 00:00:00

abstract：:Mammalian/mechanistic target of rapamycin (mTOR) is a highly conserved serine/threonine kinase that responds to environmental determinants such as growth factor concentration, nutrient availability, energy sufficiency and stress. mTOR forms two different complexes, called mTOR complex 1 (mTORC1) and mTORC2, and these ...

journal_title：Journal of biochemistry

authors： Hoshii T,Matsuda S,Hirao A

更新日期：2014-08-01 00:00:00

abstract：:A particulate enzyme preparation from Bacillus stearothermophilus synthesized 1,3-poly(glycerol phosphage) from CDPglycerol at an optimum pH of 8.0 and the reaction was stimulated by divalent cations. Km for CDPglycerol was 0.18 mM. The synthesis was inhibited by CMP, CDP, and CTP and by concentrations of CDP-glycerol...

journal_title：Journal of biochemistry

authors： Cheang OO K

更新日期：1975-03-01 00:00:00

abstract：:The accumulation of unfolded proteins in the endoplasmic reticulum (ER) under ER stress conditions activates a series of homoeostatic responses collectively termed the unfolded protein response (UPR). The UPR is unique in which the molecular mechanisms it uses to transmit signals from the ER lumen to the nucleus are c...

journal_title：Journal of biochemistry

authors： Mori K

更新日期：2009-12-01 00:00:00

abstract：:A DNA polymerase which was active with unprimed poly(dC) as a template was detected in the DNA polymerase alpha fraction from rat thymoma tissue. The activity was dependent on rGTP and associated with ribonucleotide polymerizing activity. The activity was partially sensitive to aphidicolin and dideoxy GTP and resistan...

journal_title：Journal of biochemistry

authors： Nishizawa M,Tanabe K,Matsukage A,Nakamura H,Yagura T

更新日期：1983-01-01 00:00:00

abstract：:cAMP and Ca2(+)-independent histone kinase was generated from rat liver plasma membrane in an ionic strength-dependent manner by the action of an endogenous trypsin-like protease (Hashimoto, E. et al. (1986) FEBS Lett. 200, 63-66). In addition to the effect of ionic strength, this proteolytic activation of protein kin...

journal_title：Journal of biochemistry

authors： Hashimoto E,Yamamura H

更新日期：1989-12-01 00:00:00

abstract：:Disulfide bond interchange has been pointed out as a considerable problem in preparing recombinant proteins from Escherichia coli cells. This has been reported in the system of reducing denaturation followed by a refolding process, where incorrectly folded molecules are sometimes produced. As the possibility of disulf...

journal_title：Journal of biochemistry

authors： Kimura S,Utsumi J,Yamazaki S,Shimizu H

更新日期：1988-07-01 00:00:00

abstract：:Mitochondrial DNA of an erythromycin-resistant petite mutant of yeast, E734, showed physical maps of inversion, which occurred between two cross-over sites in the fragments Hae A and Hae B. The pair of cross-over sites was inferred to be accommodated within a repeat unit of E734 mtDNA during petite mutation by joining...

journal_title：Journal of biochemistry

authors： Wakabayashi K,Furutani Y

更新日期：1984-11-01 00:00:00

abstract：:Mitochondria have two independent protein-import machineries, one in the outer membrane (the Tom system) and the other in the inner membrane (the Tim system). Here, we have characterized the initial steps of precursor import into rat liver mitoplasts. The import reaction was separated into two stages, consisting of pr...

journal_title：Journal of biochemistry

authors： Ishihara N,Komiya T,Sakaguchi M,Ito A,Mihara K

更新日期：1998-10-01 00:00:00

abstract：:Cytokine-induced neutrophil chemoattractant (CINC/Gro) is a chemotactic factor for neutrophils in the rat and a member of the CXC chemokine family. The refined three-dimensional structure of CINC/Gro was derived with the aid of heteronuclear magnetic resonance spectroscopy and a hybrid method of distance geometry and ...

journal_title：Journal of biochemistry

authors： Hanzawa H,Haruyama H,Konishi K,Watanabe K,Tsurufuji S

更新日期：1998-01-01 00:00:00

abstract：:Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

journal_title：Journal of biochemistry

authors： Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

更新日期：2001-06-01 00:00:00

abstract：:Dimeric T. flavoviridis phospholipase A2 has been studied in terms of the interaction with essential Ca2+ by equilibrium gel filtration, ultraviolet difference spectroscopy, fluorescence measurements, and chemical modifications with p-bromophenacyl bromide. The subunit bound to Ca2+ with a 1:1 molar ratio and no coope...

journal_title：Journal of biochemistry

authors： Ohno M,Honda A,Tanaka S,Mohri N,Shieh TC,Kihara H

更新日期：1984-10-01 00:00:00

abstract：:The amino acid sequence of the mature protein of Euglena gracilis cytochrome c1 was determined by sequencing of its cDNA. A cDNA expression library was constructed from Euglena poly(A)+ RNA in phage lambda gt11 and screened with an antiserum raised against cytochrome c1 polypeptide isolated from purified E. gracilis c...

journal_title：Journal of biochemistry

authors： Mukai K,Wakabayashi S,Matsubara H

更新日期：1989-09-01 00:00:00