Molecular cloning and nucleotide sequence of a cDNA encoding Euglena gracilis cytochrome c1.

abstract：:Genomic clones of UDP-glucuronosyltransferase family 1 (UGT1) were isolated from wild-type Wistar rats. The UGT1 locus spans > 120 kb and forms a gene complex. In this locus nine unique first exons encoding NH2-terminal portions of each isoform were located at intervals of approximately 10 kb and followed by only one ...

journal_title：Journal of biochemistry

authors： Emi Y,Ikushiro S,Iyanagi T

更新日期：1995-02-01 00:00:00

abstract：:We have developed three mouse monoclonal antibodies (mAb) of types IgG1 and IgG2b, i.e. anti-acetylspermine (Ac-Spm)-1 and 2 (ACSPM-1 and 2), and anti-acetylspermine (Ac-Spm)-3 (ACSPM-3), respectively, against Ac-Spm conjugated to bovine serum albumin via a heterobifunctional cross-linker, N-(gamma-maleimidobutyryloxy...

journal_title：Journal of biochemistry

authors： Fujiwara K,Kaminishi Y,Kitagawa T,Tsuru D,Yabuuchi M,Kanetake H,Nomata K

更新日期：1998-07-01 00:00:00

abstract：:Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

journal_title：Journal of biochemistry

authors： Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

更新日期：1996-04-01 00:00:00

abstract：:A cytochrome o-type oxidase from the thermophilic bacterium PS3 grown under air-limited conditions was purified by ion-exchange chromatography in the presence of a non-ionic detergent. The enzyme was composed of three subunits (60, 30, and 16 kDa) and seemed to contain two molecules of heme b as prosthetic groups. It ...

journal_title：Journal of biochemistry

authors： Sone N,Kutoh E,Sato K

更新日期：1990-04-01 00:00:00

abstract：:cAMP and Ca2(+)-independent histone kinase was generated from rat liver plasma membrane in an ionic strength-dependent manner by the action of an endogenous trypsin-like protease (Hashimoto, E. et al. (1986) FEBS Lett. 200, 63-66). In addition to the effect of ionic strength, this proteolytic activation of protein kin...

journal_title：Journal of biochemistry

authors： Hashimoto E,Yamamura H

更新日期：1989-12-01 00:00:00

abstract：:Genomic DNA libraries were screened for the human histidine-rich glycoprotein (HRG) gene and a sequence of 15,499 nucleotides was determined. The gene is composed of 7 exons and 6 introns, and all the exon-intron boundaries match the consensus GT/AG sequence for donor and acceptor splice sites. Each of cystatin-like d...

journal_title：Journal of biochemistry

authors： Wakabayashi S,Takahashi K,Koide T

更新日期：1999-03-01 00:00:00

abstract：:The complete amino acid sequence of coagulogen purified from the hemocytes of the horseshoe crab Carcinoscorpius rotundicauda was determined by characterization of the NH2-terminal sequence and the peptides generated after digestion of the protein with lysyl endopeptidase, Staphylococcal aureus protease V8 and trypsin...

journal_title：Journal of biochemistry

authors： Srimal S,Miyata T,Kawabata S,Miyata T,Iwanaga S

更新日期：1985-08-01 00:00:00

abstract：:Acyl-CoA oxidase, the initial enzyme of the peroxisomal beta-oxidation system, was purified from rat liver. The final preparation was judged to be nearly homogeneous from the results of sedimentation analysis. Ultrogel AcA-34 column chromatography, and phosphocellulose column chromatography. The molecular weight of th...

journal_title：Journal of biochemistry

authors： Osumi T,Hashimoto T,Ui N

更新日期：1980-06-01 00:00:00

abstract：:Fluorescence changes of 1-anilino-8-naphthalenesulfonic acid and 3,3'-dipropyl-2,2'-thiadicarbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increase...

journal_title：Journal of biochemistry

authors： Ueno T,Sekine T

更新日期：1978-10-01 00:00:00

abstract：:The FLI-1 transcription factor is a member of the ETS gene family, most closely related to ERG. In this study, the FLI-1 protein products were characterized using a specific monoclonal antibody previously developed against bacterially expressed protein. In the human T-cell line Jurkat, both isoforms of FLI-1, p51 and ...

journal_title：Journal of biochemistry

authors： Zhang XK,Watson DK

更新日期：2005-03-01 00:00:00

abstract：:β-lactoglobulin forms a non-native α-helix during an early stage of folding. To address the role of the non-native structure in the folding process, we designed several mutants of equine β-lactoglobulin with reduced helical propensity in the non-native helix region. One of them, A123T, showed a similar structure to th...

journal_title：Journal of biochemistry

authors： Okabe T,Miyajima T,Nakagawa K,Tsukamoto S,Fujiwara K,Ikeguchi M

更新日期：2014-11-01 00:00:00

abstract：:Angiogenesis, a formation of neovessels, is regulated by the local balance between angiogenesis stimulators and inhibitors. A number of such endogenous regulators of angiogenesis have been found in the body. Recently, vasohibin-1 (VASH1) was isolated as a negative feedback regulator of angiogenesis produced by endothe...

journal_title：Journal of biochemistry

authors： Sato Y

更新日期：2013-01-01 00:00:00

abstract：:The sigmaH of Bacillus subtilis directs transcription of a large number of early sporulation genes, whereas the principal sigma factor, sigmaA, is essential for the transcription of the genes for vegetative growth and early sporulation. We have purified sigmaA and sigmaH proteins, and characterized their properties. T...

journal_title：Journal of biochemistry

authors： Fujita M,Sadaie Y

更新日期：1998-07-01 00:00:00

abstract：:A gene for a methionine aminopeptidase (MAP; EC 3.4.11.18), which catalyzes the removal of amino-terminal methionine from the growing peptide chain on the ribosome, has been cloned from the hyperthermophilic Archaeon, Pyrococcus furiosus, by a novel method effectively using its cosmid protein library, sequenced and ex...

journal_title：Journal of biochemistry

authors： Tsunasawa S,Izu Y,Miyagi M,Kato I

更新日期：1997-10-01 00:00:00

abstract：:A novel glycosphingolipid (SEGLx) has been isolated from the plerocercoids of a tapeworm, Spirometra erinacei. From the results of compositional analysis, methylation analysis, exoglycosidase hydrolysis, acid hydrolysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance (NMR) analysis, its...

journal_title：Journal of biochemistry

authors： Kawakami Y,Nakamura K,Kojima H,Suzuki M,Inagaki F,Suzuki A,Sonoki S,Uchida A,Murata Y,Tamai Y

更新日期：1993-11-01 00:00:00

abstract：:Large amounts of extra proteins, X (in the envelope fraction) and X' (in the cytoplasmic fraction) were detected by SDS-polyacrylamide gel electrophoresis when DNA of Escherichia coli was damaged. These two proteins had the same apparent molecular weight (appproximately 40,000) and were produced under identical condit...

journal_title：Journal of biochemistry

authors： Yoda K,Nagai K,Tamura G

更新日期：1977-05-01 00:00:00

abstract：:MscL is a mechanosensitive channel that undergoes a global conformational change upon application of membrane stretching. To elucidate how the structural stability and flexibility occur, we isolated temperature-sensitive (Ts) mutants of Escherichia coli MscL that allowed cell growth at 32°C but not at 42°C. Two Ts mut...

journal_title：Journal of biochemistry

authors： Owada N,Yoshida M,Morita K,Yoshimura K

更新日期：2019-09-01 00:00:00

abstract：:Three forms of rat brain enolase (Fletcher, L., Rider, C.C., & Taylor, C.B. (1976) Biochim. Biophys. Acta 452, 245-252), which were separable by DEAE-cellulose chromatography (referred as enolase I, enolase II, and enolase III in order of the elution), were purified with a high yield by use of column chromatographies ...

journal_title：Journal of biochemistry

authors： Suzuki F,Umeda Y,Kato K

更新日期：1980-06-01 00:00:00

abstract：:Candida albicans is a commensal organism, but causes life-threatening infections in immunocompromised patients. Certain factors such as yeast-hyphae transition and hydrolytic enzymes are suggested as virulence attributes of C. albicans. Among them, 10 types of secreted aspartic protease (SAP) genes have received parti...

journal_title：Journal of biochemistry

authors： Aoki W,Kitahara N,Miura N,Morisaka H,Yamamoto Y,Kuroda K,Ueda M

更新日期：2011-10-01 00:00:00

abstract：:An ATP-dependent DNase has been purified from Thermus thermophilus HB8 by a procedure involving streptomycin precipitation, DEAE-cellulose chromatography, Sephadex G-200 gel filtration and heparin-agarose affinity chromatography. ATP-dependent DNase activity was separated into two distinct peaks, Peak A and Peak B, by...

journal_title：Journal of biochemistry

authors： Watanabe N,Umeno M,Anai M

更新日期：1983-02-01 00:00:00

abstract：:Newly recognized mammalian secretory proteins such as preprolactin are translocated across the endoplasmic reticulum (ER) in a signal recognition particle (SRP)-dependent manner. Recent studies revealed that there are two recognition steps for signal peptides during this translocation. The first step is recognition by...

journal_title：Journal of biochemistry

authors： Hatsuzawa K,Tagaya M,Mizushima S

更新日期：1997-02-01 00:00:00

abstract：:Activation of muscarinic acetylcholine receptors (mAChRs) causes the rapid release of Ca2+ from intracellular stores and a sustained influx of external Ca2+ in PC12D cells, a subline of the widely studied cell line PC12. Release of Ca2+ from intracellular stores and a sustained influx of Ca2+ are also observed followi...

journal_title：Journal of biochemistry

authors： Ebihara T,Guo F,Zhang L,Kim JY,Saffen D

更新日期：2006-03-01 00:00:00

abstract：:The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

journal_title：Journal of biochemistry

authors： Fukumori Y,Yamanaka T

更新日期：1987-02-01 00:00:00

abstract：:The stalk protein L12 is the only multiple component in 50S ribosomal subunit. In Escherichia coli, two L12 dimers bind to the C-terminal domain of L10 to form a pentameric complex, L10[(L12)(2)](2), while the recent X-ray crystallographic study and tandem MS analyses revealed the presence of a heptameric complex, L10...

journal_title：Journal of biochemistry

authors： Nomura T,Nakatsuchi M,Sugita D,Nomura M,Kaminishi T,Takemoto C,Shirouzu M,Miyoshi T,Yokoyama S,Hachimori A,Uchiumi T

更新日期：2008-11-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:The sarcoplasmic reticulum (SR) of rabbit skeletal muscle was irradiated with ultraviolet light (UV) in the presence of vanadate plus 2 mM EGTA, 10 mM MgCl2, 20% DMSO, and 50 mM PIPES (pH 6.5) at room temperature. In the presence of 100 microM vanadate, the Ca(2+)-uptake activity of SR rapidly decreased and was almost...

journal_title：Journal of biochemistry

authors： Hirose T,Yamasaki K,Yamamoto T

更新日期：1995-02-01 00:00:00

abstract：:Comparative studies of the unfolding equilibria of two homologous proteins, bovine alpha-lactalbumin and hen lysozyme, induced by treatment with guanidine hydrochloride have been made by analysis of the peptide and the aromatic circular dichroism spectra. The effect of the specific binding of Ca2+ ion by the former pr...

journal_title：Journal of biochemistry

authors： Ikeguchi M,Kuwajima K,Sugai S

更新日期：1986-04-01 00:00:00

abstract：:The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs. The in vitro synthesized RnBPs, with the respective cDNAs, formed heterodimers with porcine renin and homodimers. On comparison o...

journal_title：Journal of biochemistry

authors： Inoue H,Takahashi S,Fukui K,Miyake Y

更新日期：1991-10-01 00:00:00

abstract：:Manganese ion, like Mg2+, has been found to produce high biosynthetic activity of the unadenylylated form of glutamine synthetase obtained from Mycobacterium smegmatis, and the activity with each of these cations was decreased by the adenylylation of the enzyme. Further, the gamma-glutamyltransferase reaction was cata...

journal_title：Journal of biochemistry

authors： Matsuoka K,Kimura K

更新日期：1985-04-01 00:00:00

abstract：:The planar cell polarity (PCP) protein, Prickle (Pk), is conserved in invertebrates and vertebrates, and regulates cellular morphogenesis and movement. Vertebrate Pk consists of at least two family members, Pk1 and Pk2, both of which are expressed in the brain; however, their localization and function at synapses rema...

journal_title：Journal of biochemistry

authors： Hida Y,Fukaya M,Hagiwara A,Deguchi-Tawarada M,Yoshioka T,Kitajima I,Inoue E,Watanabe M,Ohtsuka T

更新日期：2011-06-01 00:00:00