The modulatory effect of MgATP on heterotrimeric smooth muscle myosin phosphatase activity.

abstract：:Rho family GTPases act as transducers of signals from extracellular stimuli to the cytoskeleton and gene expression. Their actions are temporal and spatial determinants for cellular functions. The cellular functions of Rho family GTPases have been studied in fibroblasts and endothelial cells, and recent advances have ...

journal_title：Journal of biochemistry

authors： Negishi M,Katoh H

更新日期：2002-08-01 00:00:00

abstract：:Coenzyme A (CoASH) is an obligate cofactor for lipids undergoing beta-oxidation in peroxisomes. Although the peroxisomal membrane appears to be impermeable to CoASH, peroxisomes contain their own pool of CoASH. It is believed that CoASH enters peroxisomes as acyl-CoAs, but it is not known how this pool is regulated. T...

journal_title：Journal of biochemistry

authors： Reilly SJ,Tillander V,Ofman R,Alexson SE,Hunt MC

更新日期：2008-11-01 00:00:00

abstract：:The effects of D-glucose anomers on the germination of dormant spores of Bacillus megaterium QM B1551 were studied, alpha-D-Glucose (1 mM) slightly initiated the germination of the dormant spores during 10 min incubation at 37 degrees C, while about 60% of the dormant spores became germinated with beta-D-glucose (1 mM...

journal_title：Journal of biochemistry

authors： Miwa I,Ishihara H,Fujii H,Nagano H,Okuda J

更新日期：1979-01-01 00:00:00

abstract：:The functions of the helix E (W97-F105), an amphiphilic lumenal 310 helix of the major antenna of photosystem II (LHCII), are still unidentified. To elucidate the roles of individual amino acid residue of the helix E, alanine scanning mutagenesis has been performed to mutate every residue of this domain to alanine. Th...

journal_title：Journal of biochemistry

authors： Liu C,Rao Y,Zhang L,Yang C

更新日期：2014-10-01 00:00:00

abstract：:The stress-related protein Stm1 interacts with ribosomes, and is implicated in repressing translation. Stm1 was previously studied both in vivo and in vitro by cell-free translation systems using crude yeast lysates, but its precise functional mechanism remains obscure. Using an in vitro reconstituted translation syst...

journal_title：Journal of biochemistry

authors： Hayashi H,Nagai R,Abe T,Wada M,Ito K,Takeuchi-Tomita N

更新日期：2018-03-01 00:00:00

abstract：:We investigated the expression and functions of circular RNA (circRNA) circNINL and miR-921 in breast cancer (BC) in this study. We found that the expression of circNINL increased while the expression of miR-921 decreased in BC tissues and cell lines, and their anomalous expressions were associated with malignant feat...

journal_title：Journal of biochemistry

authors： Xu C,Yu H,Yin X,Zhang J,Liu C,Qi H,Liu P

更新日期：2021-01-21 00:00:00

abstract：:A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...

journal_title：Journal of biochemistry

authors： Obaru K,Fukuda M,Maeda S,Shimada K

更新日期：1986-03-01 00:00:00

abstract：:Previously, we reported that proteasomes (large multi-protease complexes) are present in a latent state in a variety of eukaryotic cells, and can be activated by treatment with various compounds such as sodium dodecyl sulfate (SDS) or poly-lysine (Tanaka et al. (1988) J. Biol. Chem. 263, 16209-16217). In the present s...

journal_title：Journal of biochemistry

authors： Tanaka K,Yoshimura T,Ichihara A

更新日期：1989-09-01 00:00:00

abstract：:Isoleucyl-tRNA formation and isoleucine-dependent PPi-ATP exchange catalyzed by purified isoleucyl-tRNA synthetase [EC 6.1.1.5] of Escherichia coli were studied in the presence of various amounts of either Mg2+, Ca2+, Fe2+, Ni2+, or Cu2+. In the presence of Mg2+, isoleucine-dependent PPi-ATP exchange was observed in p...

journal_title：Journal of biochemistry

authors： Takeda Y,Ohnishi T,Ogiso Y

更新日期：1976-09-01 00:00:00

abstract：:To understand the molecular basis of the thermostability of a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1, we introduced mutations at Pro5, Pro7, Pro240 and Pro268, which are located on the surface loops of aqualysin I, by changing these amino acid residues into those found at the correspondin...

journal_title：Journal of biochemistry

authors： Sakaguchi M,Matsuzaki M,Niimiya K,Seino J,Sugahara Y,Kawakita M

更新日期：2007-02-01 00:00:00

abstract：:The ferric uptake regulator A (FurA) plays an essential role in responding to oxidative stress in mycobacteria. The genome of Mycobacterium smegmatis harbours three FurA orthologs; however, the potential cross-talk and contribution to drug resistance of different furA operon remain underdetermined. In this study, we c...

journal_title：Journal of biochemistry

authors： Gao CH,Wei WP,Tao HL,Cai LK,Jia WZ,Hu L,Yang M

更新日期：2019-09-01 00:00:00

abstract：:The SH groups of glutamine synthetase [EC 6.3.1.2] from Bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of SH groups in the molecule as well as the effect of the modification on the enzyme activity. Three SH groups per subunit were detected after co...

journal_title：Journal of biochemistry

authors： Hachimori A,Takeda A,Nagaoka T,Suzuki H,Nosoh Y,Samejima T

更新日期：1975-12-01 00:00:00

abstract：:We investigated whether transforming growth factor (TGF)-β1 promoted epithelial-mesenchymal transition (EMT) and migration of human oral squamous cell carcinoma (hOSCC) cells. Among 6 hOSCC cell lines investigated, Smad2 phosphorylation and TGF-β target genes expression were most clearly upregulated following TGF-β1 s...

journal_title：Journal of biochemistry

authors： Saito D,Kyakumoto S,Chosa N,Ibi M,Takahashi N,Okubo N,Sawada S,Ishisaki A,Kamo M

更新日期：2013-03-01 00:00:00

abstract：:Ras is a signal-transducing, guanine nucleotide-binding protein for various membrane receptors including tyrosine kinase receptors. Ras participates in the regulation of cell proliferation, differentiation, and morphology. Activated ras oncogenes have been identified in various forms of human cancer including epitheli...

journal_title：Journal of biochemistry

authors： Yamamoto T,Taya S,Kaibuchi K

更新日期：1999-11-01 00:00:00

abstract：:The effect of concentration of rabbit reticulocyte rRNAs on the translation of rabbit globin mRNAs was studied by using a rabbit reticulocyte lysate system. Globin synthesis was studied using L-[U-14C]leucine. Both the 18S and 28S rRNA inhibited globin synthesis. The 18S rRNA inhibited the synthesis of alpha-globin ch...

journal_title：Journal of biochemistry

authors： Suzuki H

更新日期：1978-04-01 00:00:00

abstract：:To obtain direct evidence for the attachment of 5SrRNA-ribosomal L5 protein particles (5SRNP) and methionine-tRNA (tRNA(met)) to methionyl-tRNA synthetase (MetRS) in the macromolecular aminoacyl-tRNA synthetase (ARS) complex of rat liver, a MetRS-5SRNP-tRNA(met) complex was dissociated from the macromolecular ARS comp...

journal_title：Journal of biochemistry

authors： Ogata K,Ohno R,Morioka S,Terao K

更新日期：1996-11-01 00:00:00

abstract：:Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry 20, 2945-2950) that these fragments can be separated by gel filtration i...

journal_title：Journal of biochemistry

authors： Hozumi T

更新日期：1981-09-01 00:00:00

abstract：:The conformational changes of ribonucleic acid induced by heat treatment were studied by measurements of fluorescence polarization, circular dichroism, and ultraviolet absorption. The fluorescence polarization of proflavine covalently bound to the 3'-end of 16S ribosomal RNA of Escherichia coli and that of ethenoadeno...

journal_title：Journal of biochemistry

authors： Kumagai JI,Ohta T,Imahori K

更新日期：1976-03-01 00:00:00

abstract：:Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each protein in different c...

journal_title：Journal of biochemistry

authors： Khademi F,Mostafaie A

更新日期：2010-05-01 00:00:00

abstract：:The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis usi...

journal_title：Journal of biochemistry

authors： Kubo T,Sasaki M,Nakamura J,Sasagawa H,Ohashi K,Takeuchi H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:A new protein factor that regulates the state of actin polymerization was purified from porcine brain by DNase I-agarose chromatography. The purified protein factor consisted of a 1 : 1 complex of 88,000 dalton polypeptide and actin. When actin was polymerized by salt in the presence of the factor, the steady-state vi...

journal_title：Journal of biochemistry

authors： Nishida E,Kuwaki T,Maekawa S,Sakai H

更新日期：1981-05-01 00:00:00

abstract：:At the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases. To understand the structural basis for the primer synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus hori...

journal_title：Journal of biochemistry

authors： Ito N,Nureki O,Shirouzu M,Yokoyama S,Hanaoka F

更新日期：2001-12-01 00:00:00

abstract：:The kinetic properties of the hydrolyses of 8-Br ATP and 8-SCH3 ATP by myosin [EC 3.6.1.3] and actomyosin were compared with those of ATP, and the following results were obtained. The Ca-NTPase activities of myosin using these two ATP analogs as substrates were smaller than that of ATPase, and the NTPase activities to...

journal_title：Journal of biochemistry

authors： Takenaka H,Ikehara M,Tonomura Y

更新日期：1976-12-01 00:00:00

abstract：:In dystrophic hamsters, increases in the levels of cathepsin B plus L and thiol proteinase inhibitor were marked in skeletal muscle, but only slight in heart muscle. The lysosomal hydrolases did not increase in parallel in dystrophic muscle: cathepsin B plus L and beta-glucuronidase increased, but cathepsin C and acid...

journal_title：Journal of biochemistry

authors： Kominami E,Bando Y,Ii K,Hizawa K,Katunuma N

更新日期：1984-12-01 00:00:00

abstract：:Crystals as large as 0.5 X 0.3 X 0.2 mm of purified arginine-transfer RNA from Escherichia coli have been prepared by a vapour diffusion method. X-ray diffraction photographs showed that the crystals gave reflections up to 3.7 A spacing. They have a trigonal space group P31 2 1 (OR P32 2 1) and cell-dimensions a=97.2,...

journal_title：Journal of biochemistry

authors： Morikawa K,Sakamaki T,Nishimura Y,Mitsui Y,Aoki K,Iitaka Y,Tsuboi M,Nishimura S

更新日期：1978-08-01 00:00:00

abstract：:The changes in the cellular concentrations of ATP, ADP, and AMP and in oxidative phosphorylation of mitochondria were investigated in the remaining liver of partially hepatectomized rabbits. The energy charge (defined as half of the average number of anhydride-bonded phosphate groups per adenosine moiety) of the liver...

journal_title：Journal of biochemistry

authors： Kamiyama Y,Ozawa K,Honjo I

更新日期：1976-10-01 00:00:00

abstract：:The binding of nuclear envelope precursor vesicles and chromatin was characterized by using an in vitro system constituted from a Xenopus egg extract and demembranated Xenopus sperm chromatin. The results of binding studies in the presence of salts, urea, and a chelator showed that the binding involves an ionic intera...

journal_title：Journal of biochemistry

authors： Imai N,Sasagawa S,Yamamoto A,Kikuchi F,Sekiya K,Ichimura T,Omata S,Horigome T

更新日期：1997-11-01 00:00:00

abstract：:Mitochondrial processing peptidase (MPP), a metalloendopeptidase consisting of alpha and beta subunits, specifically recognizes a large variety of mitochondrial precursor proteins and cleaves off the N-terminal basic matrix targeting signals (MTS). Basic residues in MTS and acidic sites in MPP are required for effecti...

journal_title：Journal of biochemistry

authors： Kitada S,Ito A

更新日期：2001-01-01 00:00:00

abstract：:Dupuytren's disease is a palmar fibromatosis associated with changes in fibroblast activity that also affect the metabolism of extracellular matrix components. In contrast to disease connected alterations in collagen and non-collagenous glycoproteins (mainly fibronectin), the metabolism of proteoglycans, being glycosa...

journal_title：Journal of biochemistry

authors： Koźma EM,Olczyk K,Wisowski G,Głowacki A,Bobiński R

更新日期：2005-04-01 00:00:00

abstract：:We have detected in rat liver cytosol three enzymes (termed C-1, C-2, and C-3) which cleaved the RNA moiety of RNA-DNA hybrid. These enzymes were separated from each other by DEAE-Sephadex and Sephadex G-200 chromatography. C-1 and C-2 specifically act on the RNA moiety of RNA-DNA hybrid, while C-3 degrades single-str...

journal_title：Journal of biochemistry

authors： Tashiro F,Ueno Y

更新日期：1978-08-01 00:00:00