A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein.

abstract：:A high mobility group (HMG) nonhistone protein fraction HMG(1 + 2), composed of HMG1 and HMG2, was prepared from pig thymus chromatin. In order to examine a possibility that the HMG(1 + 2) participates in the unwinding of the DNA double-helix, DNA hydrolysis assay systems with the endonucleases specific for single-str...

journal_title：Journal of biochemistry

authors： Yoshida M,Shimura K

更新日期：1984-01-01 00:00:00

abstract：:In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. A...

journal_title：Journal of biochemistry

authors： Umebayashi Y,Miyamoto Y,Wakita M,Kobayashi A,Nishisaka T

更新日期：2003-08-01 00:00:00

abstract：:Human HsRad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. We have characterized the self-assembly of HsRad51 by measuring the fluorescence resonance energy transfer from the fluorescein-labeled protein to the rhodamine-labeled protein. Self-as...

journal_title：Journal of biochemistry

authors： Yoshioka K,Yumoto-Yoshioka Y,Fleury F,Takahashi M

更新日期：2003-05-01 00:00:00

abstract：:During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyz...

journal_title：Journal of biochemistry

authors： Iwamuro S,Saeki M,Kato S

更新日期：1999-07-01 00:00:00

abstract：:Human platelet membrane glycoproteins IIb (GPIIb) and IIIa (GPIIIa), which have been proposed to be subunits of a receptor for fibrinogen, were purified from Triton X-100-solubilized platelet membranes by affinity chromatography on a concanavalin A (Con A)-Sepharose column followed by preparative sodium dodecyl sulfat...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-11-01 00:00:00

abstract：:Rat peritoneal macrophages were shown to have two distinct mannose/fucose/N-acetylglucosamine-specific lectins. The major lectin of 180 kDa, which is similar in size to the mannose receptor first isolated from alveolar macrophages (Wileman, T.E., Lennartz, M.R., & Stahl, P.D. (1986) Proc. Natl. Acad. Sci. U.S. 83, 250...

journal_title：Journal of biochemistry

authors： Ii M,Wada M,Kawasaki T,Yamashina I

更新日期：1988-10-01 00:00:00

abstract：:Protein kinases are essential mediators of cellular signal transduction and are often dysregulated in disease. Among these, protein tyrosine kinases (PTKs) have received specific interest due to their common roles in various diseases including cancer, and emerging observations indicating that PTK signalling pathways a...

journal_title：Journal of biochemistry

authors： Dustin CM,Heppner DE,Lin MJ,van der Vliet A

更新日期：2020-02-01 00:00:00

abstract：:Thioredoxin 1 (Trx1) is known to play an important role in protecting against cell death. However, the mechanism for control of Trx1 in cell death resulting from DNA damage has not been fully investigated. In this study, we used the DNA-damaging agent methyl methanesulfonate (MMS) to investigate the protective effects...

journal_title：Journal of biochemistry

authors： Gu L,Gao W,Yang HM,Wang BB,Wang XN,Xu J,Zhang H

更新日期：2016-01-01 00:00:00

abstract：:Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5' nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. In vitro studies have shown that SpnA is a nuclease that cleaves ssDNA and dsDNA, including the DNA backbone of neutrophil extracellular traps. S5nA was ...

journal_title：Journal of biochemistry

authors： Soh KY,Loh JMS,Proft T

更新日期：2018-08-01 00:00:00

abstract：:Using glycerol gradient centrifugation, the molecular sizes of porcine (2'-5')oligoadenylate synthetases (2-5A synthetases) were estimated. The 2-5A synthetase purified from pig spleen was about 150 kDa, while the enzyme extracted from nuclei of Newcastle disease virus-infected pig epithelial cells (SK-h) was about 20...

journal_title：Journal of biochemistry

authors： Shimizu N,Sokawa Y,Sokawa J

更新日期：1984-06-01 00:00:00

abstract：:The structure of a ferricytochrome c' extracted from Rhodospirillum rubrum has been determined at 6 A resolution by the X-ray crystallographic method. The crystals, obtained by dialyzing the protein solution against polyethylene glycol 4000, belong to the hexagonal space group P6(1). Two heavy atom derivatives were ob...

journal_title：Journal of biochemistry

authors： Yasui M,Harada S,Kai Y,Kasai N

更新日期：1985-07-01 00:00:00

abstract：:Serum mannan binding protein (MBP), a mannose/N-acetylglusosamine-specific lectin, is important in innate immunity. In order to elucidate the mechanism underlying the wide intra- and interracial variety in the MBP serum level, we have studied the transcriptional regulation of human MBP. Rapid amplification of cDNA end...

journal_title：Journal of biochemistry

authors： Naito H,Ikeda A,Hasegawa K,Oka S,Uemura K,Kawasaki N,Kawasaki T

更新日期：1999-12-01 00:00:00

abstract：:The spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, had a complex spectrum of neutral glycosphingolipids. CDS, CTS, CTeS were isolated as minor components (together they accounted for 3% of the total neutral glycolipid fraction), while CMS and the glycolipids (Lipid I, II, and III) with larger saccharide...

journal_title：Journal of biochemistry

authors： Sugita M,Yamamoto T,Masuda S,Itasaka O,Hori T

更新日期：1981-11-01 00:00:00

abstract：:Galactocerebrosidase was purified about 22,600-fold using several hydrophobic column and gel filtration steps with a 4.8% recovery, from human lymphocytes. Its specific activity was 1.54 x 10(5) nmol/h/mg with tritium-labeled galactocerebroside as the substrate in the taurocholate system. The optimal pH for galactocer...

journal_title：Journal of biochemistry

authors： Sakai N,Inui K,Midorikawa M,Okuno Y,Ueda S,Iwamatsu A,Okada S

更新日期：1994-09-01 00:00:00

abstract：:Kidney catalase [EC 1.11.1.6] was doubly labeled by injecting a mixture of [14C]-leucine and delta-amino[3H]levulinic acid (ALA) into normal and allyliso-(2-isopropyl-4-pentenoyl)urea (Sedormid)-treated rats. Shortly after the injection of a tracer dose or the precursors, catalase in microsomes showed the highest spec...

journal_title：Journal of biochemistry

authors： Higashi T,Kawamata F,Dogen M

更新日期：1976-08-01 00:00:00

abstract：:Proteins in the brush border membrane (BBM) of the midgut binding to the insecticidal Cry1Ac toxin from Bacillus thuringiensis were investigated to examine the lower sensitivity of Bombyx mori to Cry1Ac, and new aminopeptidase N that bound to Cry1Ac was discovered. DEAE chromatography of Triton X-100-soluble BBM prote...

journal_title：Journal of biochemistry

authors： Shitomi Y,Hayakawa T,Hossain DM,Higuchi M,Miyamoto K,Nakanishi K,Sato R,Hori H

更新日期：2006-02-01 00:00:00

abstract：:The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite's survival. We cloned and studied the biochemical properties of plant-type ferredoxin (Fd) and Fd-NADP+ reductase (FNR), a redox system that p...

journal_title：Journal of biochemistry

authors： Kimata-Ariga Y,Kurisu G,Kusunoki M,Aoki S,Sato D,Kobayashi T,Kita K,Horii T,Hase T

更新日期：2007-03-01 00:00:00

abstract：:Serum at 5 to 10% is required for maintenance of functional adult rat hepatocytes in primary culture. One effect of the serum is to induce attachment and spreading of hepatocytes on plates as monolayers. Another role is to maintain cell viability for over 2 days. For the first effect, serum could be replaced completel...

journal_title：Journal of biochemistry

authors： Horiuti Y,Nakamura T,Ichihara A

更新日期：1982-12-01 00:00:00

abstract：:The kinetic specificities of BPN' and Carlsberg subtilisins [EC 3.4.21.14] were examined with various nucleus-substituted derivatives of Nalpha-acetylated aromatic amino acid methyl esters for mapping their hydrophobic binding sites in comparison with that of alpha-chymotrypsin. The Carlsberg enzyme was generally much...

journal_title：Journal of biochemistry

authors： Karasaki Y,Ohno M

更新日期：1978-09-01 00:00:00

abstract：:Ras is a signal-transducing, guanine nucleotide-binding protein for various membrane receptors including tyrosine kinase receptors. Ras participates in the regulation of cell proliferation, differentiation, and morphology. Activated ras oncogenes have been identified in various forms of human cancer including epitheli...

journal_title：Journal of biochemistry

authors： Yamamoto T,Taya S,Kaibuchi K

更新日期：1999-11-01 00:00:00

abstract：:Mitochondria play many critical roles in cells, not only by supplying energy, but also by supplying metabolites, buffering Ca2+ levels and regulating apoptosis. During oocyte maturation and subsequent embryo development, mitochondria change their morphology by membrane fusion and fission, and coordinately undergo mult...

journal_title：Journal of biochemistry

authors： Udagawa O,Ishihara N

更新日期：2020-03-01 00:00:00

abstract：:Low molecular weight RNAs were isolated from nuclei of the cellular slime mold Dictyostelium discoideum AX-3. Analysis of the RNAs by polyacrylamide gel electrophoresis showed that the vegetative cell nuclei contained, besides tRNA (Dd1), 5S RNA (Dd4), and 5.8S RNA (Dd7), at least 7 small RNA species (Dd3, Dd5, Dd6, D...

journal_title：Journal of biochemistry

authors： Takeishi K,Kaneda S

更新日期：1981-08-01 00:00:00

abstract：:The Tetrahymena group I intron ribozyme folds into a complex three dimensional structure for performing the self-splicing reaction. Catalysis depends on its core structure comprising two helical domains, P4-P6 and P3-P7. The two domains are joined by three sets of conserved base-triple(s) and other tertiary interactio...

journal_title：Journal of biochemistry

authors： Ohki Y,Ikawa Y,Shiraishi H,Inoue T

更新日期：2002-11-01 00:00:00

abstract：:Both beef heart cytochrome oxidase and bacteriorhodopsin of Halobacterium halobium were reconstituted into liposomes by the sonication-cholate dialysis method. The proteoliposomes showed the respiratory control ratio of 4.2, and steady-state illumination of the vesicles lead to the 2.7-fold stimulation of the oxidase ...

journal_title：Journal of biochemistry

authors： Miki T,Orii Y,Mukohata Y

更新日期：1987-07-01 00:00:00

abstract：:The light protein components of silk fibroin were analyzed by Western blotting using polyclonal antibodies against two synthetic peptides that are segment of the light (L-) chain [Yamaguchi et al. (1989) J. Mol. Biol. 210, 127-139] and P25 [Chevillard et al. (1986) Sericologia 26, 435-449], respectively, and against t...

journal_title：Journal of biochemistry

authors： Tanaka K,Mori K,Mizuno S

更新日期：1993-07-01 00:00:00

abstract：:Midkine (MK) and pleiotrophin (PTN) constitute a new family of heparin-binding growth factors. Extraembryonic membranes and the placenta of the mouse expressed MK mRNA at 11.5 days gestation. While the MK mRNA level in extraembryonic membranes decreased during embryogenesis, that in the placenta remained unchanged. Im...

journal_title：Journal of biochemistry

authors： Obama H,Tsutsui J,Ozawa M,Yoshida H,Yoshida Y,Osame M,Muramatsu T

更新日期：1995-07-01 00:00:00

abstract：:The complete amino acid sequence of coagulogen purified from the hemocytes of the horseshoe crab Carcinoscorpius rotundicauda was determined by characterization of the NH2-terminal sequence and the peptides generated after digestion of the protein with lysyl endopeptidase, Staphylococcal aureus protease V8 and trypsin...

journal_title：Journal of biochemistry

authors： Srimal S,Miyata T,Kawabata S,Miyata T,Iwanaga S

更新日期：1985-08-01 00:00:00

abstract：:The role of HSDJ, a human homolog of bacterial DnaJ and yeast YDJ1p/MAS5, in mitochondrial protein import was examined. Recombinant HSDJ was purified and an antibody was prepared. HSDJ mRNA was heat-induced in cultured cells. In pulse-labeling and chase experiments using COS-7 cells, the endogenous HSDJ homolog was pr...

journal_title：Journal of biochemistry

authors： Kanazawa M,Terada K,Kato S,Mori M

更新日期：1997-05-01 00:00:00

abstract：:Centromere protein B (CENP-B) is one of the centromere DNA binding proteins constituting centromeric heterochromatin of human chromosomes. This protein was originally identified as the target antigen in autoimmune disease patients (often with scleroderma). In this study, we cloned a human CENP-B cDNA which was longer ...

journal_title：Journal of biochemistry

authors： Sugimoto K,Muro Y,Himeno M

更新日期：1992-04-01 00:00:00

abstract：:1. The distribution of ribosomal protein S1 in subcellular fractions of E. coli was determined by radioimmunoassay. It was found that about 70%, 20% and 10% of protein S1 were present in the high salt (1.0 M NH4Cl)-washed ribosomes, the ribosomal wash and the S100 fraction, respectively. 2. Protein S1 was purified fro...

journal_title：Journal of biochemistry

authors： Yokota T,Arai K,Kaziro Y

更新日期：1979-12-01 00:00:00