Abstract:
:The sensitivity and specificity of two influenza C virus assays, solid-phase and overlay assays, were investigated using naturally occurring 9-O-acetylated GD(3), rat serum glycoproteins containing 60% of N-acetyl-9-O-acetylneuraminic acid, and synthetically O-acetylated sialylated compounds. The sensitivity of the solid-phase assay was higher for glycoproteins containing N-acetyl-9-O-acetylneuraminic acid than for gangliosides, and also differed for various 9-O-acetylated gangliosides. The overlay assay was less sensitive for all glycoconjugates tested. For virus recognition the presentation of the sialic acid within the molecule and the structure of the sialic acid are essential. Investigation of gangliosides from human melanomas and normal skin with the influenza C virus assay showed an increase of O-acetylation of sialic acids in most tumour samples and the occurrence of several O-acetylated gangliosides.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Hubl U,Ishida H,Kiso M,Hasegawa A,Schauer Rdoi
10.1093/oxfordjournals.jbchem.a022693keywords:
subject
Has Abstractpub_date
2000-06-01 00:00:00pages
1021-31issue
6eissn
0021-924Xissn
1756-2651journal_volume
127pub_type
杂志文章abstract::Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
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更新日期:1999-05-01 00:00:00
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更新日期:2002-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131809
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022553
更新日期:1999-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132999
更新日期:1980-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a125022
更新日期:1995-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1977-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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更新日期:1999-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-09-01 00:00:00
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更新日期:2004-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134252
更新日期:1983-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122462
更新日期:1988-08-01 00:00:00
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更新日期:1990-10-01 00:00:00