Abstract:
:The properties of phospholipase A1 (PLA1) obtained from the white muscle of bonito, Euthynnus pelamis (Linnaeus), were examined. The PLA1 activity had a pH optimum from 6.5 to 7.0 for phosphatidylcholine (PC), and calcium ion was not required. The optimum temperature was from 20 to 30 degrees C. When a fatty alcohol was used as an acceptor, a wax ester was produced by transferring a fatty acid at the sn-1 position of the donor's PC. The maximum production of lysophosphatidylcholine was shifted by 0.5 pH units to the acidic side and the pH optimum of wax ester synthesis was from 6.0 to 6.5. The synthesis was independent of calcium ion and Coenzyme A. The transacylation was also observed when 1-lyso-2-acyl-sn-glycero-3-phosphocholine was used as an acceptor. Fatty acid at the sn-1 position of the donor PC was transferred to the unoccupied hydroxy group of the acceptor at the sn-1 position. When 2,3-dipalmitoyl-sn-glycero-1-phosphocholine was used as the acyl donor, a similar amount of palmitic acid was transferred as in the case of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. However, 1-acyl-2-lyso-sn -glycero-3-phosphocholine, a positional isomer, was a poor acceptor. These results indicate that the transacylation by the PLA1 from bonito muscle is not stereospecific, but is position-specific both for the acyl donor and acceptor.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Hirano K,Tanaka A,Yoshizumi K,Tanaka T,Satouchi Kdoi
10.1093/oxfordjournals.jbchem.a021876subject
Has Abstractpub_date
1997-12-01 00:00:00pages
1160-6issue
6eissn
0021-924Xissn
1756-2651journal_volume
122pub_type
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journal_title:Journal of biochemistry
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pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1984-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121868
更新日期:1986-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131268
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更新日期:1996-01-01 00:00:00
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更新日期:2003-02-01 00:00:00
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更新日期:1975-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-05-01 00:00:00