Abstract:
:The region containing the RNA polymerase alpha subunit (RNAPalpha) gene (rpoA) and the ribosomal protein genes of a thermophilic eubacterial strain, Thermus thermophilus (Tt) HB8, was cloned from a genomic DNA library by Southern hybridization. The gene order in this region is rpl36-rps13-rps11-rps4-rpoA-rpl17, which is identical to that in some other eubacteria. The rpoA gene encodes a 315 amino acid residue protein with a molecular weight of 35,013, the amino acid sequence showing 42% identity to that of Escherichia coli (Ec). From the results of comparison of the amino acid sequence and the predicted secondary structure of the C-terminal domain of Tt RNAPalpha (Tt alphaCTD) with those of Ec, the overall folding is expected to be similar. However, amino acid residues Asn268 and Cys269 in Ec alphaCTD, which are essential for its interaction with DNA or regulatory proteins, were replaced by His and Ser, respectively, in Tt alphaCTD. By means of a T7-based expression system in Ec cells, Tt RNAPalpha was overexpressed and purified. The high thermostability of Tt RNAPalpha was demonstrated by the CD spectra.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Wada T,Yamazaki T,Kuramitsu S,Kyogoku Ydoi
10.1093/oxfordjournals.jbchem.a022251subject
Has Abstractpub_date
1999-01-01 00:00:00pages
143-50issue
1eissn
0021-924Xissn
1756-2651journal_volume
125pub_type
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journal_title:Journal of biochemistry
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134301
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132790
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123154
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132537
更新日期:1979-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124236
更新日期:1993-11-01 00:00:00
abstract::Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...
journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
doi:10.1093/oxfordjournals.jbchem.a022590
更新日期:2000-02-01 00:00:00