Fibrinopeptides A and B of Japanese monkey (Macaca fuscata) and patas monkey (Erythrocebus patas): their amino acid sequences, restricted mutations, and a molecular phylogeny for macaques, guenons, and baboons.

abstract：:The patterns of myosin isozymes in embryonic and adult chicken pectoralis muscle were examined by electrophoresis in a non-denaturing gel system (pyrophosphate acrylamide gel electrophoresis), and both light chains and heavy chains of embryonic and adult myosin isozymes were compared. In pyrophosphate acrylamide gel e...

journal_title：Journal of biochemistry

authors： Takano-Ohmuro H,Obinata T,Masaki T,Mikawa T

更新日期：1982-04-01 00:00:00

abstract：:HSP47 is a stress protein (heat shock protein) which resides in the endoplasmic reticulum, and is postulated to function as a collagen-specific molecular chaperone. To elucidate the role of HSP47 in procollagen biosynthesis, we have established human embryonic kidney 293 cell lines, which were stably transfected with ...

journal_title：Journal of biochemistry

authors： Hosokawa N,Hohenadl C,Satoh M,Kühn K,Nagata K

更新日期：1998-09-01 00:00:00

abstract：:tRNA(guanosine-2'-)-methyltransferases (Gm-methylases) isolated from extreme thermophiles, Thermus thermophilus strains HB 27 and HB 8, methylate the 2'-OH of the G18 ribose of the GG sequence in the D loop of tRNA, by recognizing the D "loop-stem" structure as a minimal requirement. To examine the role of the consens...

journal_title：Journal of biochemistry

authors： Hori H,Saneyoshi M,Kumagai I,Miura K,Watanabe K

更新日期：1989-11-01 00:00:00

abstract：:Rat liver glucocorticoid receptor was partially purified and characterized for its hormone binding using selenite. Selenite at very low concentrations irreversibly inhibited the hormone binding. The concentration for half maximal inhibition was approximately 2.8 microM. The inhibition was restored by dithiothreitol. T...

journal_title：Journal of biochemistry

authors： Tashima Y,Terui M,Itoh H,Mizunuma H,Kobayashi R,Marumo F

更新日期：1989-03-01 00:00:00

abstract：:A simple and rapid method to remove myosin light chain phosphatase from gizzard myosin using hydroxyapatite chromatography was developed. Stable phosphorylated myosin without light chain phosphatase and kinase was also prepared by the same procedure. A convenient method using the urea gel system to detect minor contam...

journal_title：Journal of biochemistry

authors： Nakamura S,Nonomura Y

更新日期：1984-08-01 00:00:00

abstract：:D-type cyclin-dependent kinases (Cdk4 and Cdk6) regulate the G1 to S phase progression of the mammalian cell cycle. It has been suggested that Cdk4 and Cdk6 may have distinct functions in vivo, even though they are indistinguishable biochemically. Here we show that although these Cdks phosphorylate multiple residues i...

journal_title：Journal of biochemistry

authors： Takaki T,Fukasawa K,Suzuki-Takahashi I,Semba K,Kitagawa M,Taya Y,Hirai H

更新日期：2005-03-01 00:00:00

abstract：:Eukaryotic typical 2-Cys type peroxiredoxin (Prx) is inactivated by hyperoxidation of the peroxidatic cysteine to a sulphinic acid in a catalytic cycle-dependent manner. This inactivation process has been well documented for cytosolic isoforms of Prx. However, such a hyperoxidative inactivation has not fully been inve...

journal_title：Journal of biochemistry

authors： Ikeda Y,Nakano M,Ihara H,Ito R,Taniguchi N,Fujii J

更新日期：2011-04-01 00:00:00

abstract：:Stable cell lines expressing the gastric proton pump alpha- and/or beta-subunits were constructed. The cell line co-expressing the alpha- and beta-subunits showed inward Rb(+) transport, which was activated by Rb(+) in a concentration-dependent manner. In the alpha+beta-expressing cell line, rapid recovery of intracel...

journal_title：Journal of biochemistry

authors： Kimura T,Yoshida A,Tabuchi Y,Ikari A,Takeguchi N,Asano S

更新日期：2002-06-01 00:00:00

abstract：:We examined the effects of growth factors on intracellular free Mg2+ concentrations ([Mg2+]i) in single Swiss 3T3 fibroblasts, using microfluorometry of a Mg(2+)-sensitive dye mag-fura-2. We had already noted an increase in [Mg2+]i after exposure to bombesin for 30-60 min [Ishijima, S., Sonoda, T., & Tatibana, M. (199...

journal_title：Journal of biochemistry

authors： Ishijima S,Tatibana M

更新日期：1994-04-01 00:00:00

abstract：:Phospholipase A2 inhibitor (PLI) was purified from the blood plasma of Chinese Mamushi, Agkistrodon blomhoffii siniticus, by sequential chromatography on Sephadex G-200, Mono Q, and Blue-Sepharose CL-6B columns. The purified PLI was a glycoprotein with an apparent molecular mass of 75 kDa and was composed of a single ...

journal_title：Journal of biochemistry

authors： Ohkura N,Inoue S,Ikeda K,Hayashi K

更新日期：1993-04-01 00:00:00

abstract：:Anti-angiotensin I IgG in serum was measured by immune complex transfer enzyme immunoassays using angiotensin I conjugates prepared by two different methods. In the first method, angiotensin I was conjugated to dinitrophenyl bovine serum albumin and beta-D-galactosidase through covalent links. Anti-angiotensin I IgG i...

journal_title：Journal of biochemistry

authors： Kohno T,Yamaguchi K,Ishikawa E

更新日期：1990-11-01 00:00:00

abstract：:Protein carbonyls are reported to increase in aging and in pathologies such as Alzheimer's disease and ischemic injury. Detailed study of this important issue has, however, been hampered by lack of an appropriate method to identify individual carbonylated proteins. We describe here an immunoblot method to identify ind...

journal_title：Journal of biochemistry

authors： Nakamura A,Goto S

更新日期：1996-04-01 00:00:00

abstract：:Proton translocating ATPase (F0F1) from bovine heart mitochondria was reconstituted into planar phospholipid bilayers, and its electrogenicity was directly demonstrated. The F0F1 ATPase was solubilized using 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonic acid (CHAPS) as a detergent followed by sucrose densi...

journal_title：Journal of biochemistry

authors： Muneyuki E,Ohno K,Kagawa Y,Hirata H

更新日期：1987-12-01 00:00:00

abstract：:The cytoplasmic peptide elongation factor, EF-1 alpha, is anchored at the endoplasmic reticulum membrane by phosphatidylinositol via ethanolamine bridging presumably to Asp306 of the protein. ...

journal_title：Journal of biochemistry

authors： Hayashi Y,Urade R,Utsumi S,Kito M

更新日期：1989-10-01 00:00:00

abstract：:It is known that ABO blood group substances in human erythrocyte membranes are sphingoglycolipids, but recently several authors have reported that the glycoproteins of the erythrocyte membranes also have ABO blood group activities in addition to MN blood group activities and virus hemagglutination inhibitor activity. ...

journal_title：Journal of biochemistry

authors： Yamato K,Handa S,Yamakawa T

更新日期：1975-12-01 00:00:00

abstract：:alpha-Actinin purified from chicken gizzard smooth muscle was characterized in comparison with alpha-actinins from chicken striated muscles, or fast-skeletal muscle, slow-skeletal muscle, and cardiac muscle. The gizzard alpha-actinin molecule consisted of two apparently identical subunits with a molecular weight of 10...

journal_title：Journal of biochemistry

authors： Endo T,Masaki T

更新日期：1982-11-01 00:00:00

abstract：:We designed a simple procedure for the purification of peptidylarginine deiminase, which catalyzes the deimination of arginyl residues in protein, from rabbit skeletal muscle using substrate affinity chromatography. Of the immobilized substrate ligands tested, i.e. protamine and soybean trypsin inhibitor (Kunitz) (STI...

journal_title：Journal of biochemistry

authors： Takahara H,Okamoto H,Sugawara K

更新日期：1986-05-01 00:00:00

abstract：:Deleting the gene for small RNA GcvB in Esherichia coli was found to increase the sensitivity to several aminoglycoside antibiotics, such as neomycin, streptomycin, kanamycin, kasugamycin and spectinomycin, at low concentrations. GcvB, conserved in gram-negative enteric bacteria, is known to negatively control the exp...

journal_title：Journal of biochemistry

authors： Muto A,Goto S,Kurita D,Ushida C,Himeno H

更新日期：2020-11-10 00:00:00

abstract：:Accumulation of pyrophosphate induced by acetate administration was investigated in rat liver in situ and in perfused rat liver. Intraperitoneal injection of acetate into rats increased the pyrophosphate concentration in the liver to about 2 mumol/g liver, which was 200 times that in control liver. Perfusion of liver ...

journal_title：Journal of biochemistry

authors： Yamada T,Inoue T,Nishida T,Furuya E,Tagawa K

更新日期：1988-11-01 00:00:00

abstract：:We have reported that Erbin expression was down-regulated in the Jurkat leukaemia T lymphocytes treated with the recombinant soluble tumour necrosis factor-related apoptosis-inducing ligand (rsTRAIL). Herein, we studied the expression and the regulation of Erbin and its binding partner, ErbB2, in the MCF-7 breast canc...

journal_title：Journal of biochemistry

authors： Liu N,Zhang J,Zhang J,Liu S,Liu Y,Zheng D

更新日期：2008-06-01 00:00:00

abstract：:[3H]Methyl group incorporation and histamine secretion in rat mast cells induced by anti-IgE and con A were strongly inhibited by trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester (GMCHA-OPhBut), a strong and specific inhibitor for pH 7 tryptase (Muramatsu et al. (1988) Biol. Chem. Hoppe-Seyle...

journal_title：Journal of biochemistry

authors： Takei M,Matumoto T,Endo K,Muramatu M

更新日期：1989-02-01 00:00:00

abstract：:By use of purified RNA polymerase II, it was demonstrated that S-II, a stimulatory protein of RNA polymerase II, enhanced the frequency of initiation of transcription from discrete sites on the promoter region of the silk fibroin gene integrated in a supercoiled plasmid DNA in the presence of manganese. In the absence...

journal_title：Journal of biochemistry

authors： Sekimizu K,Horikoshi M,Natori S

更新日期：1984-12-01 00:00:00

abstract：:The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the pr...

journal_title：Journal of biochemistry

authors： Shiwa Y,Yoshikawa H,Tanaka T,Ogura M

更新日期：2015-05-01 00:00:00

abstract：:To study the effect of cross-linking of membrane proteins on vesiculation of human erythrocytes by dimyristoylphosphatidylcholine (DMPC), red cells were treated with diamide at atmospheric pressure or 100 MPa and then incubated with DMPC in buffers of pH 6.5-8.5. Irrespective of buffer pH, the amount of released vesic...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Yamada S,Kimoto E

更新日期：1994-04-01 00:00:00

abstract：:In the rat, DNA carrying a cluster of the genes for tRNAAsp, tRNAGly, and tRNAGlu, aligned in that order, is repeated about 10 times. Seven DNA clones corresponding to the independent repeating units were isolated from a rat gene library. Nucleotide sequence analysis of these clones revealed the presence of a fourth t...

journal_title：Journal of biochemistry

authors： Shibuya K,Noguchi S,Yamaki M,Nishimura S,Sekiya T

更新日期：1985-06-01 00:00:00

abstract：:A heat-labile, non-dialyzable factor(s) in soluble fractions from Escherichia coli strains and Bacillus subtilis was found to incorporate the radioactivity of [14C]glutamic acid into 95 degrees C CCl3COOH-insoluble fraction. Incorporation catalyzed by a partially purified factor from E. coli B required ATP, Mg2+, tRNA...

journal_title：Journal of biochemistry

authors： Hashizume S,Honjoh T,Shoji M

更新日期：1983-04-01 00:00:00

abstract：:Fibronectin contains at least two distinct oligopeptide sequences serving as signals for the interaction with cell surface adhesion receptors termed integrins. One of these sequences, Arg-Gly-Asp-Ser (RGDS) tetrapeptide, was shown to be transferred to a truncated form of Staphylococcal IgG-binding protein (hereafter r...

journal_title：Journal of biochemistry

authors： Maeda T,Oyama R,Titani K,Sekiguchi K

更新日期：1993-01-01 00:00:00

abstract：:Chain elongation of fusaric acid and related compounds in the presence of rat liver preparations was investigated by gas chromatography-mass spectrometry. The mitochondrial fraction catalyzed the elongation of the CoA esters of fusaric acid and 5-butyl-2-pyrimidinecarboxylic acid utilizing acetyl-CoA as a C2 donor. Th...

journal_title：Journal of biochemistry

authors： Mizugaki M,Sagi M,Yamanaka H,Takayama H,Ishibashi M,Miyazaki H

更新日期：1986-02-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs), or prion diseases, are fatal infectious neurodegenerative disorders. Their causative agents are prions, which are composed of disease-associated forms of prion protein (PrP(Sc)). Naturally occurring cases of TSEs are found in several mammalian species including humans,...

journal_title：Journal of biochemistry

authors： Hagiwara K,Hara H,Hanada K

更新日期：2013-02-01 00:00:00

abstract：:Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...

journal_title：Journal of biochemistry

authors： Yamauchi T,Moroishi T

更新日期：2020-11-23 00:00:00