Abstract:
:Resistant strains for trimethoprim, a potent inhibitor of dihydrofolate reductase, were obtained by transforming the ligated products of Escherichia coli K12 DNA and plasmid pBR322 BamH I fragments. The strains carry a 13.6 kbp plasmid, pTP1, which contains the trimethoprim- and ampicillin-resistance determinant genes. The trimethoprim-resistance determinant gene was estimated to consist of more than 500 nucleotides and less than 1,500 nucleotides and was restricted by EcoR I and Sal I. Trimethoprim-, ampicillin-, and tetracycline-resistant plasmids were made in the following way, and the resultant plasmids contained a unique EcoR I "insertional inactivation" site for trimethoprim resistance: the DNA sequences extraneous to the determinant gene of the trimethoprim resistance on BamH I fragment of pTP 1 were eliminated by digestion with a double-strand-specific exonuclease BAL 31, and the resultant fragments were ligated with pBR 322 which had been digested by EcoR I and a single-strand-specific nuclease S1. The strains carrying pTP 1 or trimethoprim-resistant plasmids produced about 10 times more dihydrofolate reductase than control strains. The enhancement of the enzyme production, which is due to an increase in the copy number of the enzyme gene, seems to be responsible for the trimethoprim resistance of the transformed cells.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Iwakura M,Shimura Y,Tsuda Kdoi
10.1093/oxfordjournals.jbchem.a133804subject
Has Abstractpub_date
1982-04-01 00:00:00pages
1205-12issue
4eissn
0021-924Xissn
1756-2651journal_volume
91pub_type
杂志文章abstract::Incubation of Sarcophaga fat bodies in buffered insect saline resulted in simultaneous activation of multiple defense protein genes [Sugiyama, H. and Natori, S. (1991) Eur. J. Biochem. 200, 495-500]. In this study, we demonstrated that the addition of both adenosine and homocysteine thiolactone to this medium abolishe...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134236
更新日期:1983-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133480
更新日期:1981-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/jb/101.3.643
更新日期:1987-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132349
更新日期:1979-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022196
更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1979-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122596
更新日期:1988-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135260
更新日期:1985-07-01 00:00:00
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pub_type: 杂志文章
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doi:10.1093/oxfordjournals.jbchem.a123120
更新日期:1990-05-01 00:00:00
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更新日期:2001-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1981-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1997-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122212
更新日期:1988-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134058
更新日期:1982-11-01 00:00:00
abstract::Sulfate-reducing bacteria, Desulfovibrio vulgaris, strain Miyazaki, were grown on either sulfate, sulfite, or thiosulfate as the terminal electron acceptor. Better growth was observed on sulfite and less growth on thiosulfate than on sulfate. Enzyme levels of adenylylsulfate (APS) reductase [EC 1.8.99.2], reductant-ac...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130985
更新日期:1975-11-01 00:00:00
abstract::Histidine and its derivatives increased rabbit muscle fructose 1,6-bisphosphatase activity at neutral pH with positive cooperativity. In the presence of histidine and carnosine the optimum pH shifted from pH 8.0 to 7.4. The cooperative response of the enzyme to AMP and fructose 1,6-bisphosphate was observed in the pre...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132723
更新日期:1980-01-01 00:00:00