Interaction of myosin.ADP.fluorometal complexes with fluorescent probes and direct observation using quick-freeze deep-etch electron microscopy.

abstract：:The readthrough of premature termination codon (PTC) by ribosome sometimes produces full-length proteins. We previously reported a readthrough of PTC of glycosyltransferase gene B4GALNT1 with hereditary spastic paraplegia (HSP). Here we featured the readthrough of B4GALNT1 of two mutants, M4 and M2 with PTC by immunob...

journal_title：Journal of biochemistry

authors： Yesmin F,Bhuiyan RH,Ohmi Y,Ohkawa Y,Tajima O,Okajima T,Furukawa K,Furukawa K

更新日期：2020-08-01 00:00:00

abstract：:An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance sp...

journal_title：Journal of biochemistry

authors： Waki H,Murata A,Kon K,Maruyama K,Kimura S,Ogura H,Ando S

更新日期：1993-04-01 00:00:00

abstract：:Subtilisin BPN' was chemically converted to thiolsubtilisin and the interaction of this modified enzyme with Streptomyces subtilisin inhibitor (SSI) was examined. SSI competitively inhibited the esterolytic activity of thiolsubtilisin toward p-nitrophenyl acetate with a K1 value of 1.3 X 10(-5) M at pH 7.5 Spectrophot...

journal_title：Journal of biochemistry

authors： Fujiwara K,Inouye K,Tonomura B,Murao S,Tsuru D

更新日期：1977-07-01 00:00:00

abstract：:Physicochemical and biological properties of recombinant human erythropoietin (rhEPO) were compared with human urinary erythropoietin (uEPO). uEPO and rhEPO were purified to apparent homogeneity from the urine of patients with aplastic anemia and from the conditioned medium of Chinese hamster ovary (CHO) cells transfe...

journal_title：Journal of biochemistry

authors： Imai N,Kawamura A,Higuchi M,Oh-eda M,Orita T,Kawaguchi T,Ochi N

更新日期：1990-03-01 00:00:00

abstract：:The effect of polyamines on F1-ATPase catalyzed reactions has been studied through the use of submitochondrial particles and F1-ATPase. ATP degradation catalyzed by submitochondrial particles and F1-ATPase was inhibited by spermine and spermidine. Spermine's inhibition was much greater than spermidine's effect. In con...

journal_title：Journal of biochemistry

authors： Igarashi K,Kashiwagi K,Kobayashi H,Ohnishi R,Kakegawa T,Nagasu A,Hirose S

更新日期：1989-08-01 00:00:00

abstract：:Peptide:N-glycanase (PNGase) is the enzyme responsible for de-N-glycosylation of misfolded glycoproteins in the cytosol. Here, we report the molecular identification and characterization of PNGase (png-1, F56G4.5) from Caenorhabditis elegans. This enzyme released both high mannose- and complex-type N-glycans from glyc...

journal_title：Journal of biochemistry

authors： Kato T,Kawahara A,Ashida H,Yamamoto K

更新日期：2007-08-01 00:00:00

abstract：:The Escherichia coli strain carrying pTP 6-10 which was constructed in our previous work (Iwakura, M., et al. (1983) J. Biochem. 93, 927-930) produces more than 400-fold dihydrofolate reductase as compared with the strain without the plasmid. Dihydrofolate reductase was highly purified from the cell-free extract of th...

journal_title：Journal of biochemistry

authors： Iwakura M,Shimura Y,Sakai T,Tsuda K

更新日期：1983-09-01 00:00:00

abstract：:Kinetic studies of the two beta-glucosidase isozymes, GB-1 and GB-2, which were from the culture filtrate of a phytopathogenic fungus Pyricularia oryzae, revaled that the latter isozyme was an allosteric protein with two substrate binding sites. The homotropic effects of o- and m-nitrophenyl-beta-D-glucosides on GB-2 ...

journal_title：Journal of biochemistry

authors： Hirayama T,Nagayama H,Matsuda K

更新日期：1980-04-01 00:00:00

abstract：:The transcription and replication of influenza RNA can be studied in vitro by the reconstitution of functional ribonucleoprotein (RNP) complex from viral core proteins including the RNA polymerase (complex of three P protein subunits) and nucleoprotein (NP), and model templates. Here, two different core protein prepar...

journal_title：Journal of biochemistry

authors： Seong BL,Kobayashi M,Nagata K,Brownlee GG,Ishihama A

更新日期：1992-04-01 00:00:00

abstract：:1. The actin-activated Mg2+-ATPase activity of gizzard HMM increased in proportion to the square of the extent of LC phosphorylation. This result indicates that the LCs of HMM are randomly phosphorylated, and the phosphorylation of both heads of HMM is required for the activation of HMM Mg2+-ATPase by F-actin. 2. In 7...

journal_title：Journal of biochemistry

authors： Ikebe M,Ogihara S,Tonomura Y

更新日期：1982-05-01 00:00:00

abstract：:Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 45...

journal_title：Journal of biochemistry

authors： Ohe T,Watanabe Y

更新日期：1979-07-01 00:00:00

abstract：:The activity of ornithine decarboxylase (ODC) in rabbit costal chondrocytes in culture markedly increased after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 h after PTH addition. The effect of PTH was dose-dependent both in the presence and absence of fetal calf serum. Addition of cycloheximide or ...

journal_title：Journal of biochemistry

authors： Takigawa M,Watanabe R,Ishida H,Asada A,Suzuki F

更新日期：1979-01-01 00:00:00

abstract：:The ferric uptake regulator A (FurA) plays an essential role in responding to oxidative stress in mycobacteria. The genome of Mycobacterium smegmatis harbours three FurA orthologs; however, the potential cross-talk and contribution to drug resistance of different furA operon remain underdetermined. In this study, we c...

journal_title：Journal of biochemistry

authors： Gao CH,Wei WP,Tao HL,Cai LK,Jia WZ,Hu L,Yang M

更新日期：2019-09-01 00:00:00

abstract：:The nucleotide sequence of the mRNA for NADPH-cytochrome P-450 reductase from rabbit liver was determined from a full-length cDNA clone (pFP105). The clone contains 2,269 nucleotides complementary to rabbit liver reductase mRNA. The single open reading frame of 2,037 nucleotides codes for a 679-amino acid polypeptide ...

journal_title：Journal of biochemistry

authors： Katagiri M,Murakami H,Yabusaki Y,Sugiyama T,Okamoto M,Yamano T,Ohkawa H

更新日期：1986-10-01 00:00:00

abstract：:1. The distribution of ribosomal protein S1 in subcellular fractions of E. coli was determined by radioimmunoassay. It was found that about 70%, 20% and 10% of protein S1 were present in the high salt (1.0 M NH4Cl)-washed ribosomes, the ribosomal wash and the S100 fraction, respectively. 2. Protein S1 was purified fro...

journal_title：Journal of biochemistry

authors： Yokota T,Arai K,Kaziro Y

更新日期：1979-12-01 00:00:00

abstract：:Chain elongation of fusaric acid and related compounds in the presence of rat liver preparations was investigated by gas chromatography-mass spectrometry. The mitochondrial fraction catalyzed the elongation of the CoA esters of fusaric acid and 5-butyl-2-pyrimidinecarboxylic acid utilizing acetyl-CoA as a C2 donor. Th...

journal_title：Journal of biochemistry

authors： Mizugaki M,Sagi M,Yamanaka H,Takayama H,Ishibashi M,Miyazaki H

更新日期：1986-02-01 00:00:00

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:The pH-activity curve of heavy meromyosin ATPase [EC 3.6.1.3] was measured at various temperatures. The pH-activity curve at higher temperatures showed a maximum at low pH and a minimum at pH 7 to 8 as has been already reported. At lower temperatures it was sigmoidal in shape, similar to a simple dissociation curve of...

journal_title：Journal of biochemistry

authors： Ishigami F,Morita F

更新日期：1977-02-01 00:00:00

abstract：:Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the delta pH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dic...

journal_title：Journal of biochemistry

authors： Moriyama Y,Takano T,Ohkuma S

更新日期：1982-10-01 00:00:00

abstract：:Plant cell-wall arabinoxylans have a complex structure that requires the action of a pool of debranching (arabinofuranosidases) and depolymerizing enzymes (endo-xylanase). Two Aspergillus nidulans strains over-secreting endo-xylanase and arabinofuranosidase were inoculated in defined 2% maltose-minimum medium resultin...

journal_title：Journal of biochemistry

authors： Damásio AR,Pessela BC,da Silva TM,Guimarães LH,Jorge JA,Guisán JM,Polizeli Mde L

更新日期：2013-09-01 00:00:00

abstract：:A protease activity specific to spermatogenous histones was found in the egg extract of sea urchin. The enzyme responsible for this activity, named SPKK protease because of its substrate specificity, was purified as a monomeric 28 kDa protein. SPKK protease activity is inhibited by leupeptin and is specific to the rep...

journal_title：Journal of biochemistry

authors： Suzuki M,Sugiura M,Ebashi S

更新日期：1990-09-01 00:00:00

abstract：:[3H]Methyl group incorporation and histamine secretion in rat mast cells induced by anti-IgE and con A were strongly inhibited by trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester (GMCHA-OPhBut), a strong and specific inhibitor for pH 7 tryptase (Muramatsu et al. (1988) Biol. Chem. Hoppe-Seyle...

journal_title：Journal of biochemistry

authors： Takei M,Matumoto T,Endo K,Muramatu M

更新日期：1989-02-01 00:00:00

abstract：:Angiogenesis, a formation of neovessels, is regulated by the local balance between angiogenesis stimulators and inhibitors. A number of such endogenous regulators of angiogenesis have been found in the body. Recently, vasohibin-1 (VASH1) was isolated as a negative feedback regulator of angiogenesis produced by endothe...

journal_title：Journal of biochemistry

authors： Sato Y

更新日期：2013-01-01 00:00:00

abstract：:The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth...

journal_title：Journal of biochemistry

authors： Ishihama A,Enami M,Nishijima Y,Fukui T,Ohtsuka E,Ikehara M

更新日期：1980-03-01 00:00:00

abstract：:The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...

journal_title：Journal of biochemistry

authors： Hayashi T,Nakamura T,Hori H,Nagai Y

更新日期：1980-03-01 00:00:00

abstract：:A number of gene mutations are detected in cells derived from human cancer tissues, but roles of these mutations in cancer cell development are largely unknown. We examined G364R mutation of MCM4 detected in human skin cancer cells. Formation of MCM4/6/7 complex is not affected by the mutation. Consistent with this no...

journal_title：Journal of biochemistry

authors： Ishimi Y,Irie D

更新日期：2015-06-01 00:00:00

abstract：:To enhance the heat stability of the CutA1 protein from Escherichia coli (EcCutA1) so that it has comparable stability to CutA1 from Pyrococcus horikoshii with a denaturation temperature (T(d)) of 150°C, we used the Stability Profile of Mutant Protein (SPMP) to examine the structure-sequence (3D-1D) compatibility betw...

journal_title：Journal of biochemistry

authors： Matsuura Y,Ota M,Tanaka T,Takehira M,Ogasahara K,Bagautdinov B,Kunishima N,Yutani K

更新日期：2010-10-01 00:00:00

abstract：:A completely sodium dodecyl sulfate-insoluble protein was obtained from plasmodia of Physarum polycephalum. The amino acid composition of this protein was very similar to that of connectin, an elastic protein of muscle. When the protein suspension was treated with 6 M guanidine-HCl in the presence of 0.1 M 2-mercaptoe...

journal_title：Journal of biochemistry

authors： Ozaki K,Maruyama K

更新日期：1980-09-01 00:00:00

abstract：:The receptor protein for the mitochondrial protein precursor synthesized in the cytosol was extensively purified from the mitochondrial membrane fraction by affinity column chromatography using a synthetic peptide containing the extrapeptide of ornithine aminotransferase as a ligand. The purified fraction contained tw...

journal_title：Journal of biochemistry

authors： Ono H,Tuboi S

更新日期：1990-06-01 00:00:00

abstract：:When the competitive inhibitor benzeneboronic acid (BBA) forms a complex with alpha-chymotrypsin [EC 3.4.21.1] protons are released in the acidic pH region. The proton release can be measured by a difference potentiometric technique. The proton release is also observed in chymotrypsinogen A but not in TRCK-, DIP-, and...

journal_title：Journal of biochemistry

authors： Hanai K

更新日期：1976-01-01 00:00:00