Remarkable improvement in the heat stability of CutA1 from Escherichia coli by rational protein design.

abstract：:Serum-free culture medium collected from primary monolayer cultures of human articular chondrocytes was found to inhibit human urokinase [EC 3.4.21.31] activity. Although chondrocyte culture medium contained a small amount of endothelial-type plasminogen activator inhibitor which could be demonstrated by reverse fibri...

journal_title：Journal of biochemistry

authors： Yamada H,Stephens RW,Nakagawa T,McNicol D

更新日期：1988-12-01 00:00:00

abstract：:Haematopoiesis and blood vessel formation are closely associated, with several molecules employed by both systems. Recently, vasohibin-1 (VASH1), an endothelium-derived negative feedback regulator of angiogenesis, has been isolated and characterized. VASH1 is induced by VEGF or bFGF in endothelial cells (ECs) and inhi...

journal_title：Journal of biochemistry

authors： Naito H,Kidoya H,Sato Y,Takakura N

更新日期：2009-05-01 00:00:00

abstract：:A novel immortalized rheumatoid fibroblast-like synoviocyte (FLS) line, MH7A, was established by stably transfecting FLS cells with SV40 T antigen gene. MH7A cells expressed SV40-specific small t and large T antigens as well as an elevated level of p53 protein. They have already reached over 150 population doublings t...

journal_title：Journal of biochemistry

authors： Miyazawa K,Mori A,Okudaira H

更新日期：1998-12-01 00:00:00

abstract：:The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rate...

journal_title：Journal of biochemistry

authors： Shiota M,Chinzei F,Moriyama M,Kimura K,Sugano T

更新日期：1995-05-01 00:00:00

abstract：:The complete amino acid sequence of ribonuclease N1 (RNase N1), a guanine-specific ribonuclease from a fungus, Neurospora crassa, was determined by conventional protein sequencing, using peptide fragments obtained by tryptic digestion of cyanogen bromide-treated RNase N1 and by Staphylococcus aureus V8 protease digest...

journal_title：Journal of biochemistry

authors： Takahashi K

更新日期：1988-09-01 00:00:00

abstract：:Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating in normal somatic cells...

journal_title：Journal of biochemistry

authors： Nozawa K,Kurumiya Y,Yamamoto A,Isobe Y,Suzuki M,Yoshida S

更新日期：1999-08-01 00:00:00

abstract：:The alkalophile NADH dehydrogenase (NADH: 2,6-dichlorophenolindophenol oxidoreductase) [EC 1.6.99.3] consists of two identical subunits of 65 kDa, and each subunit contains the catalytic and liposome-binding regions. On treatment with trypsin, the polypeptide exhibiting the liposome-binding property in one of the subu...

journal_title：Journal of biochemistry

authors： Xu XM,Kanaya S,Koyama N,Sekiguchi T,Nosoh Y,Ohashi S,Tsuda K

更新日期：1989-04-01 00:00:00

abstract：:We previously reported that DNAs directly applicable to restriction analyses and transformation of Escherichia coli can be extracted from fungi and yeasts by use of anhydrous hydrogen fluoride (HF) under a mild condition, 5 min at 0 degrees C [Oshiro, S., Katsura, N., Kitada, K., and Gunge, N. (1987) FEBS Lett. 220, 3...

journal_title：Journal of biochemistry

authors： Oshiro S,Katsura N

更新日期：1995-03-01 00:00:00

abstract：:The pcd gene from Flavobacterium lutescens IFO3084 encoding Delta'-piperideine-6-carboxylate dehydrogenase (PCD) was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence of PCD from F. lutescens IFO3084 showed strong similarity to that from Streptomyces clavuligerus. The molecular mass...

journal_title：Journal of biochemistry

authors： Fujii T,Narita T,Agematu H,Agata N,Isshiki K

更新日期：2000-12-01 00:00:00

abstract：:Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

journal_title：Journal of biochemistry

authors： Kobayashi T,Simizu T

更新日期：1976-06-01 00:00:00

abstract：:Deoxyhypusine synthase catalyzes the NAD+-dependent formation of deoxyhypusine in the eIF-5A precursor protein by transferring the 4-aminobutyl moiety of spermidine. This enzyme has recently been shown to be essential for cell viability and growth of yeast [Sasaki, K., Abid, M.R., and Miyazaki, M. (1996) FEBS Lett. 38...

journal_title：Journal of biochemistry

authors： Abid MR,Sasaki K,Titani K,Miyazaki M

更新日期：1997-04-01 00:00:00

abstract：:Genomic clones encoding the Drosophila aldolase gene were isolated and the organization of the gene was determined. The protein-coding region spanning nearly 3.5 kb consists of five coding exons (exon 2, 3, 4 alpha, 4 beta, and 4 gamma). The insect exon 2 corresponds to exons 2 to 7 of vertebrate aldolase genes and th...

journal_title：Journal of biochemistry

authors： Kai T,Sugimoto Y,Kusakabe T,Zhang R,Koga K,Hori K

更新日期：1992-11-01 00:00:00

abstract：:Anti-angiotensin I IgG in serum was measured by immune complex transfer enzyme immunoassays using angiotensin I conjugates prepared by two different methods. In the first method, angiotensin I was conjugated to dinitrophenyl bovine serum albumin and beta-D-galactosidase through covalent links. Anti-angiotensin I IgG i...

journal_title：Journal of biochemistry

authors： Kohno T,Yamaguchi K,Ishikawa E

更新日期：1990-11-01 00:00:00

abstract：:In order to determine the mode of action of cytostatic 9-beta-D-xylofuranosyladenine (xylo-A), the inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate (xylo-ATP) on DNA-dependent RNA polymerases I and II purified from cherry salmon (Oncorhynchus masou) liver nuclei were studied. This nucleotide showed ...

journal_title：Journal of biochemistry

authors： Nakayama C,Saneyoshi M

更新日期：1985-05-01 00:00:00

abstract：:Catalase from Aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. The enzyme had a molecular weight of 385,000 as estimated from sedimentation measurements. Carbohydrate analyses showed that the catalase was a glycoprotein containing a...

journal_title：Journal of biochemistry

authors： Kikuchi-Torii K,Hayashi S,Nakamoto H,Nakamura S

更新日期：1982-11-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into gamma-cyclodextrin (gamma-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase with those of other typical CGTases revealed that several amino acids are deleted or substituted with others at se...

journal_title：Journal of biochemistry

authors： Nakagawa Y,Takada M,Ogawa K,Hatada Y,Horikoshi K

更新日期：2006-09-01 00:00:00

abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

journal_title：Journal of biochemistry

authors： Takeda A,Hirano K,Shiroya Y,Samejima T

更新日期：1983-04-01 00:00:00

abstract：:The mitogen-activated protein kinase (MAPK) signaling pathway, which is conserved from yeast to humans, is activated in response to a variety of extra- and intracellular stimuli, and plays key roles in multiple cellular processes, including proliferation, differentiation, and apoptosis. The MAPK pathway transmits its ...

journal_title：Journal of biochemistry

authors： Yoshioka K

更新日期：2004-06-01 00:00:00

abstract：:We prepared a streptavidin-based neoglycoprotein which carries more than 140 GT1b oligosaccharides. GT1b oligosaccharides were covalently coupled to streptavidin by reductive amination, yielding a monomer form of streptavidin carrying 13 oligosaccharides. The monomer form of glycosylated streptavidin was polymerized w...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Suzuki M,Crocker PR,Suzuki A

更新日期：1998-03-01 00:00:00

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:Connectin (titin) links the Z line to the myosin filament in sarcomeres of vertebrate skeletal muscle. An 800 kDa fragment of alpha-connectin runs from the Z line up to the N2 line region in the I band, and the following beta-connectin portion runs up to the edge of the M line on the myosin filament in chicken breast ...

journal_title：Journal of biochemistry

authors： Kawamura Y,Kume H,Itoh Y,Ohtsuka S,Kimura S,Maruyama K

更新日期：1995-01-01 00:00:00

abstract：:Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

journal_title：Journal of biochemistry

authors： Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

更新日期：1981-11-01 00:00:00

abstract：:The complete peptide map of purified recombinant human interleukin 5 (rhIL-5) was determined to verify its primary structure, glycosylation sites, and disulfide bonding structure. Each peptide fragment generated by Achromobacter protease I (API) digestion was purified and characterized by amino acid analysis and amino...

journal_title：Journal of biochemistry

authors： Minamitake Y,Kodama S,Katayama T,Adachi H,Tanaka S,Tsujimoto M

更新日期：1990-02-01 00:00:00

abstract：:We evaluated the role of cytosolic phospholipase A2 (PLA2) in the exocytosis of amylase from parotid acinar cells. The exocytosis stimulated by isoproterenol was dose-dependently inhibited by bromoenol lactone (BEL), a potent suicide inhibitor of Ca2+-independent cytosolic PLA2. The IC50 value of BEL was approximately...

journal_title：Journal of biochemistry

authors： Takuma T,Ichida T

更新日期：1997-06-01 00:00:00

abstract：:Ion-exchange column-purified I-protein was labeled by fluorescein isothiocyanate (FITC) at an equimolar ratio. When FITC-labeled I-protein was reacted with glycerinated myofibrils of chicken breast muscle in a phosphate-buffered saline, fluorescence was observed at the A-band and/or the Z-line of the sarcomere. Howeve...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1988-02-01 00:00:00

abstract：:SpoIIGA is a novel type of membrane-associated aspartic protease that responds to a signal from the forespore by cleaving Pro-σ(E) in the mother cell during sporulation of Bacillus subtilis. Very little is known about how SpoIIGA recognizes Pro-σ(E). By co-expressing proteins in Escherichia coli, it was shown that cha...

journal_title：Journal of biochemistry

authors： Imamura D,Kuwana R,Kroos L,Feig M,Takamatsu H,Watabe K

更新日期：2011-06-01 00:00:00

abstract：:Histidinol dehydrogenase (HDH), a Zn-metalloenzyme, produces His from histidinol through two successive oxidation reactions with NAD+ as a coenzyme. A mutation, His261-->Asn, caused the complete loss of the Zn, thereby inactivating the enzyme, without significant structural perturbation. The ability to oxidize an inte...

journal_title：Journal of biochemistry

authors： Nagai A,Ohta D

更新日期：1994-01-01 00:00:00

abstract：:Induction of cytosolic aspartate aminotransferase (cAspAT) was observed in rat liver on administration of a high-protein diet, or glucagon and during fasting. The enzyme activity in the liver of rats given 80% protein diet or glucagon injection during starvation increased to 2- to 2.4-fold that in the liver of rats ma...

journal_title：Journal of biochemistry

authors： Horio Y,Tanaka T,Taketoshi M,Uno T,Wada H

更新日期：1988-05-01 00:00:00

abstract：:The effect of concentration of rabbit reticulocyte rRNAs on the translation of rabbit globin mRNAs was studied by using a rabbit reticulocyte lysate system. Globin synthesis was studied using L-[U-14C]leucine. Both the 18S and 28S rRNA inhibited globin synthesis. The 18S rRNA inhibited the synthesis of alpha-globin ch...

journal_title：Journal of biochemistry

authors： Suzuki H

更新日期：1978-04-01 00:00:00

abstract：:HPC-1/syntaxin 1A (HPC-1), which plays an important role in vesicular transport to the plasma membrane, possesses a hydrophobic sequence at its C terminus. When expressed from cDNA in COS cells, wild-type HPC-1 was localized in the Golgi complex and the plasma membrane. Truncation of the hydrophobic domain resulted in...

journal_title：Journal of biochemistry

authors： Masaki R,Yamamoto A,Akagawa K,Tashiro Y

更新日期：1998-08-01 00:00:00