Abstract:
:The ATP requirement for two steps of DNA replication, the synthesis and subsequent joining of Okazaki fragments, was investigated by using isolated HeLa cell nuclei. Among adenine nucleotides tested, high levels of dATP and ADP stimulated DNA synthesis. In the presence of high levels of ATP, the addition of high levels of dATP or ADP resulted in about 70% inhibition of DNa synthesis. The optimal concentration of ATP for the stimulation of DNA synthesis varied depending on the magnesium ion concentration. When the molar ratio of magnesium ion to ATP was approximately 1, maximal stimulation was attained. Product analysis by sedimentation in an alkaline sucrose gradient revealed that both Okazaki fragments and high molecular weight DNA were synthesized in the presence of high levels of ATP, whereas in the case of dATP and ADP, little high molecular weight DNA was synthesized. The ability to synthesize high molecular weight DNA was restored to nuclei by adding low levels of ATP in the presence of high levels of ADP but not of dATP.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Enomoto T,Tanuma S,Yamada MAdoi
10.1093/oxfordjournals.jbchem.a133262subject
Has Abstractpub_date
1981-03-01 00:00:00pages
801-7issue
3eissn
0021-924Xissn
1756-2651journal_volume
89pub_type
杂志文章abstract::By using commercially available anhydrous hydrazine in the gas-phase, mucin-type oligosaccharides were released from porcine gastric mucin (PGM) and bovine fetuin. The data indicated that a certain amount of the oligosaccharides from PGM were further degraded. Despite this, the HPLC elution profile of the anthranilic ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123308
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
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更新日期:1989-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132326
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135172
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133646
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123573
更新日期:1991-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022176
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121889
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131051
更新日期:1976-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133183
更新日期:1981-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135526
更新日期:1986-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021886
更新日期:1997-12-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1993-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1995-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021940
更新日期:1998-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1976-03-01 00:00:00