Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. IV. Reconstitution from minimal- and high-dose images of the actin-tropomyosin-myosin subfragment-1 complex.

abstract：:MscL is a mechanosensitive channel that undergoes a global conformational change upon application of membrane stretching. To elucidate how the structural stability and flexibility occur, we isolated temperature-sensitive (Ts) mutants of Escherichia coli MscL that allowed cell growth at 32°C but not at 42°C. Two Ts mut...

journal_title：Journal of biochemistry

authors： Owada N,Yoshida M,Morita K,Yoshimura K

更新日期：2019-09-01 00:00:00

abstract：:An ATP-dependent DNase has been purified from Thermus thermophilus HB8 by a procedure involving streptomycin precipitation, DEAE-cellulose chromatography, Sephadex G-200 gel filtration and heparin-agarose affinity chromatography. ATP-dependent DNase activity was separated into two distinct peaks, Peak A and Peak B, by...

journal_title：Journal of biochemistry

authors： Watanabe N,Umeno M,Anai M

更新日期：1983-02-01 00:00:00

abstract：:An improved procedure for the purification of a lipid-containing acidophilic thermophilic bacteriophage, phiNS11, is described. The procedure involves Cs2SO4 discontinuous density gradient, Cs2SO4 equilibrium density gradient and sucrose gradient centrifugations. The phage has an icosahedral shape, 75 nm in diameter, ...

journal_title：Journal of biochemistry

authors： Sakaki Y,Yamada K,Oshima M,Oshima T

更新日期：1977-11-01 00:00:00

abstract：:An inhibitor of blood coagulation, a new protein with an apparent molecular weight of 34,000 and an isoelectric point of 4.9, was purified from human placental tissue by EDTA extraction. Five cDNA clones were isolated from the human placental lambda gt11 cDNA library using the mouse monoclonal antibody raised against ...

journal_title：Journal of biochemistry

authors： Iwasaki A,Suda M,Nakao H,Nagoya T,Saino Y,Arai K,Mizoguchi T,Sato F,Yoshizaki H,Hirata M

更新日期：1987-11-01 00:00:00

abstract：:In the rat liver, two mRNAs for serine:pyruvate (or alanine:glyoxylate) aminotransferase are generated from a single gene by alternative transcription initiation. The longer mRNA encodes a precursor of a mitochondrial enzyme that has a mitochondrial targeting signal at the N-terminus and is translocated into mitochond...

journal_title：Journal of biochemistry

authors： Oda T,Uchida C,Miura S

更新日期：2000-04-01 00:00:00

abstract：:Archaeal geranylgeranyl diphosphate (GGPP) synthase catalyzes the consecutive condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to produce GGPP with significant amounts of intermediates. To obtain information about the amino acids involved in the condensation and the release of intermediates, we ...

journal_title：Journal of biochemistry

authors： Ohnuma S,Hemmi H,Ohto C,Nakane H,Nishino T

更新日期：1997-04-01 00:00:00

abstract：:We identified a protein with a molecular weight of about 44 kDa in an extract of rat regenerating liver. This protein was undetectable in normal, sham-operated, and completely regenerated liver. We also purified this 44-kDa protein from an extract of rat liver remnant after partial hepatectomy. The partial amino acid ...

journal_title：Journal of biochemistry

authors： Tanahashi T,Suzuki M,Itoh N,Mitsui Y

更新日期：1995-08-01 00:00:00

abstract：:RPE65 is a membrane-associated retinoid isomerase involved in the visual cycle responsible for sustaining vision. Many mutations in the human RPE65 gene are associated with distinct forms of retinal degenerative diseases. The pathogenic mechanisms for most of these mutations remain poorly understood. Here, we show tha...

journal_title：Journal of biochemistry

authors： Li S,Hu J,Jin RJ,Aiyar A,Jacobson SG,Bok D,Jin M

更新日期：2015-08-01 00:00:00

abstract：:Kinetic studies of the reduction of plastocyanin from spinach chloroplasts by ascorbate were carried out using a stopped flow apparatus. The reduction of spinach plastocyanin by ascorbate followed first-order kinetics in the reductant with a rate constant k = 415 M-1.S-1 [20 degrees C, 0.1 M NaCl, pH 8.0 (10 mM Tris-H...

journal_title：Journal of biochemistry

authors： Takabe T,Niwa S,Ishikawa H,Miyakawa M

更新日期：1980-01-01 00:00:00

abstract：:Chondroitin sulphate proteoglycan 4 (CSPG4) is a cell surface proteoglycan highly expressed by tumour, perivascular and oligodendrocyte cells and known to be involved cell adhesion and migration. This study showed that CSPG4 was present as a proteoglycan on the cell surface of two melanoma cell lines, MM200 and Me1007...

journal_title：Journal of biochemistry

authors： Tang F,Lord MS,Stallcup WB,Whitelock JM

更新日期：2018-05-01 00:00:00

abstract：:A dinuclear La3+ complex hydrolyzes the phosphodiester bond in diribonucleotides efficiently under mild conditions. The rate of hydrolysis is remarkably accelerated on the complex formation between La3+ and TPHP (TPHP = N,N,N',N'-tetrakis [(2-pyridyl)methyl]-2-hydroxy-1,3-diaminopropane), and the highest activity is a...

journal_title：Journal of biochemistry

authors： Yashiro M,Ishikubo A,Komiyama M

更新日期：1996-12-01 00:00:00

abstract：:Actin-microtubule crosstalk is implicated in the formation of cellular protrusions, but the mechanism remains unclear. In this study, we examined the regulation of cell protrusion involving a ubiquitously expressed microtubule-associated protein (MAP) 4, and its superfamily proteins, neuronal MAP2 and tau. Fluorescenc...

journal_title：Journal of biochemistry

authors： Doki C,Nishida K,Saito S,Shiga M,Ogara H,Kuramoto A,Kuragano M,Nozumi M,Igarashi M,Nakagawa H,Kotani S,Tokuraku K

更新日期：2020-09-01 00:00:00

abstract：:Tryptophan hydroxylase from mouse mastocytoma P-815 is activated by ethylenediaminetetraacetate (EDTA). The activation proceeds in a pH-, temperature-, and time-dependent manner and leads to a 30-fold higher activity at maximum. The optimal EDTA concentration is 10 microns. The activation requires solely EDTA with des...

journal_title：Journal of biochemistry

authors： Yanagisawa M,Hasegawa H,Ichiyama A

更新日期：1982-08-01 00:00:00

abstract：:Thyrotropin releasing hormone (TRH) caused significant breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) in GH3 cells, but vasoactive intestinal peptide (VIP) did not. However, VIP enhanced the TRH-induced hydrolysis of PIP2, the conversion of phosphatidylinositol 4-phosphate (PIP) to PIP2 and the accumulation...

journal_title：Journal of biochemistry

authors： Suzuki R,Takenawa T

更新日期：1987-09-01 00:00:00

abstract：:Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the reversed arrangement of the variable domains of the heavy and light chains ...

journal_title：Journal of biochemistry

authors： Merk H,Stiege W,Tsumoto K,Kumagai I,Erdmann VA

更新日期：1999-02-01 00:00:00

abstract：:Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

journal_title：Journal of biochemistry

authors： Morishima N,Ikai A

更新日期：1987-12-01 00:00:00

abstract：:Whether or not the thermotropic change at about 18 degrees C in the physical state of Ca2+-ATPase protein molecules of sarcoplasmic reticulum membranes could be transmitted to lipids through protein-lipid interactions was investigated using a spin-label technique. Fatty acid spin labels were used to probe the bulk mem...

journal_title：Journal of biochemistry

authors： Kirino Y,Higashi K,Matsui M,Shimizu H

更新日期：1981-03-01 00:00:00

abstract：:Incubation of quiescent cultures of Swiss 3T3 cells with epidermal growth factor (EGF) caused an increase in c-myc mRNA. Under these conditions, EGF did not induce phosphoinositide turnover, formation of diacylglycerol, formation of inositol tris-, bis-, and monophosphates, protein kinase C activation, or Ca2+ mobiliz...

journal_title：Journal of biochemistry

authors： Tsuda T,Hamamori Y,Fukumoto Y,Kaibuchi K,Takai Y

更新日期：1986-12-01 00:00:00

abstract：:In the rat, DNA carrying a cluster of the genes for tRNAAsp, tRNAGly, and tRNAGlu, aligned in that order, is repeated about 10 times. Seven DNA clones corresponding to the independent repeating units were isolated from a rat gene library. Nucleotide sequence analysis of these clones revealed the presence of a fourth t...

journal_title：Journal of biochemistry

authors： Shibuya K,Noguchi S,Yamaki M,Nishimura S,Sekiya T

更新日期：1985-06-01 00:00:00

abstract：:Trypsin was used as a probe of F-actin conformation. F-actin is known to be refractory to proteolysis [Jacobson, G.R. and Rosenbusch, J.P. (1976) Proc. Natl. Acad. Sci. U.S. 73, 2742-2746]. However, here it was found that F-actin could also be digested by trypsin to a 33-kDa fragment (like G-actin) when free MgADP is ...

journal_title：Journal of biochemistry

authors： Hozumi T

更新日期：1988-08-01 00:00:00

abstract：:1. The actin-activated Mg2+-ATPase activity of gizzard HMM increased in proportion to the square of the extent of LC phosphorylation. This result indicates that the LCs of HMM are randomly phosphorylated, and the phosphorylation of both heads of HMM is required for the activation of HMM Mg2+-ATPase by F-actin. 2. In 7...

journal_title：Journal of biochemistry

authors： Ikebe M,Ogihara S,Tonomura Y

更新日期：1982-05-01 00:00:00

abstract：:Human, bovine, and mouse C1q, a subcomponent of the first complement component, were purified, and both globular (GF) and collagen-like fragments (CLF) were isolated from human and bovine C1q. Antisera were produced in rabbits with these C1q or fragments, and F(ab')2 of immunoglobulin G (IgG) was purified from the ant...

journal_title：Journal of biochemistry

authors： Yonemasu K,Sasaki T,Shimizu A

更新日期：1983-04-01 00:00:00

abstract：:Pull-down assay and co-immunoprecipitation of cell extracts in which the integrase or reverse transcriptase of Moloney murine leukemia virus was transiently expressed showed that both enzymes interacted with PML proteins. In infected cells, interaction between the integrase and PML was also observed. Transient express...

journal_title：Journal of biochemistry

authors： Okino Y,Inayoshi Y,Kojima Y,Kidani S,Kaneoka H,Honkawa A,Higuchi H,Nishijima K,Miyake K,Iijima S

更新日期：2012-08-01 00:00:00

abstract：:Methyl-coenzyme M reductase isoenzyme I from the methanogenic Archaeon, Methanobacterium thermoautotrophicum (strain Marburg), was crystallized by vapor diffusion methods. Crystal form M obtained with 2-methyl-2,4-pentanediol as the precipitant displayed space group P2(1), with unit cell parameters of a=83.2 A, b=117....

journal_title：Journal of biochemistry

authors： Shima S,Goubeaud M,Vinzenz D,Thauer RK,Ermler U

更新日期：1997-05-01 00:00:00

abstract：:We synthesized a series of non-ionic detergents, alkyl thiomaltosides, and investigated their properties and usefulness. We solubilized membrane proteins of Vibrio parahaemolyticus using the detergents. With octyl thiomaltoside, nonyl thiomaltoside, decyl thiomaltoside, or undecyl thiomaltoside, we observed satisfacto...

journal_title：Journal of biochemistry

authors： Izawa S,Sakai-Tomita Y,Kinomura K,Kitazawa S,Tsuda M,Tsuchiya T

更新日期：1993-05-01 00:00:00

abstract：:The Escherichia coli phenylalanine-sensitive DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate) synthetase (aroG product) is one of the DAHP synthetase isozymes that catalyze the first committed step in the biosynthesis of aromatic amino acids and vitamins. Through target-directed mutagenesis of the cloned aroG on a p...

journal_title：Journal of biochemistry

authors： Ger YM,Chen SL,Chiang HJ,Shiuan D

更新日期：1994-11-01 00:00:00

abstract：:Acid ceramidase (ACDase) metabolizes ceramide to sphingosine, leading to sphingosine 1-phosphate production. Reportedly, ACDase has been upregulated in prostate cancer. However, its regulatory mechanism remains unclear. LNCaP (androgen-sensitive prostate cancer cell line) but not PC3 and DU-145, (androgen-unresponsive...

journal_title：Journal of biochemistry

authors： Mizutani N,Inoue M,Omori Y,Ito H,Tamiya-Koizumi K,Takagi A,Kojima T,Nakamura M,Iwaki S,Nakatochi M,Suzuki M,Nozawa Y,Murate T

更新日期：2015-10-01 00:00:00

abstract：:Poly(8-oxyinosinic acid) (poly O8I), was synthesized by polymerizing 8-oxyinosine diphosphate using the enzyme polynucleotide phosphorylase (PNPase). The polymer formed a 1 : 1 hybrid with polycytidylic acid (poly C). The hybrid was found to induce the production of interferon in the brain of white albino mice and pro...

journal_title：Journal of biochemistry

authors： Folayan JO

更新日期：1982-09-01 00:00:00

abstract：:Had-1, which was isolated from mouse FM3A carcinoma cells, was a non-permissive mutant cell line to Newcastle disease virus infection. Comparative study of the asparagine-linked sugar chains of the surface glycoproteins of the mutant and its parental cells revealed that galactosylation of the complex-type sugar chains...

journal_title：Journal of biochemistry

authors： Hara T,Endo T,Furukawa K,Kawakita M,Kobata A

更新日期：1989-08-01 00:00:00

abstract：:Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...

journal_title：Journal of biochemistry

authors： Yamauchi T,Moroishi T

更新日期：2020-11-23 00:00:00