Specificity of rat liver cathepsin D.

abstract：:To investigate the effect of plasminogen on cartilage catabolism, we assessed collagen degradation in rabbit articular cartilage explants treated with or without plasminogen and interleukin-1alpha (IL-1alpha). The combination of IL-1 alpha and plasminogen induced rapid collagen degradation, amounting to more than 60% ...

journal_title：Journal of biochemistry

authors： Saito S,Katoh M,Masumoto M,Matsumoto S,Masuho Y

更新日期：1997-07-01 00:00:00

abstract：:LukF and Hlg2 of staphylococcal gamma-hemolysin assemble into hetero-oligomeric pores on human red blood cells (HRBC). Here, we demonstrate, using a single-molecule imaging technique, that a W177T/R198T mutant of LukF, which exhibits no binding activity toward phosphatidylcholine, could form intermediate oligomers wit...

journal_title：Journal of biochemistry

authors： Monma N,Nguyen VT,Kaneko J,Higuchi H,Kamio Y

更新日期：2004-10-01 00:00:00

abstract：:A cysteine protease inhibitor with an apparent Mr = 12,600, designated limulus (L)-cystatin, was isolated from hemocyte lysates of the Japanese horseshoe crab (Tachypleus tridentatus), using two steps of chromatography, including dextran sulfate-agarose, and carboxymethylated papain-agarose. L-cystatin inhibits amidol...

journal_title：Journal of biochemistry

authors： Agarwala KL,Kawabata S,Hirata M,Miyagi M,Tsunasawa S,Iwanaga S

更新日期：1996-01-01 00:00:00

abstract：:Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...

journal_title：Journal of biochemistry

authors： Suhara W,Yoneyama M,Yonekawa H,Fujita T

更新日期：1996-02-01 00:00:00

abstract：:The characteristics of the androgen receptor in the cytoplasmic fraction of Shionogi carcinoma 115 were studied in vitro by means of charcoal adsorption assay, sucrose density gradient centrifugation, and polyacrylamide gel electrophoresis. The equilibrium dissociation constant for [3H]dihydrotestosterone (Kd = (2-5) ...

journal_title：Journal of biochemistry

authors： Nohno T

更新日期：1981-02-01 00:00:00

abstract：:The interactions of benzeneboronic acid (BBA) as a transition state analog with subtilisin (EC 3.4.21.4) and with alpha-chymotrypsin (EC 3.4.21.1) were investigated kinetically by the temperature-jump method using pH indicators. For both enzymes, the concentration dependence of the relaxation time was consistent with...

journal_title：Journal of biochemistry

authors： Nakatani H,Hanai K,Uehara Y,Hiromi K

更新日期：1975-04-01 00:00:00

abstract：:The complete amino acid sequence of coagulogen purified from the hemocytes of the horseshoe crab Carcinoscorpius rotundicauda was determined by characterization of the NH2-terminal sequence and the peptides generated after digestion of the protein with lysyl endopeptidase, Staphylococcal aureus protease V8 and trypsin...

journal_title：Journal of biochemistry

authors： Srimal S,Miyata T,Kawabata S,Miyata T,Iwanaga S

更新日期：1985-08-01 00:00:00

abstract：:Rat gene for renin-binding protein (RnBP) was shown to be expressed in the kidney, adrenal gland, brain, lung, spleen, ovary, testis, and heart. On sodium depletion and captopril administration, the rat showed a marked increase in the adrenal RnBP mRNA level and a slight decrease in the kidney RnBP mRNA level. In two-...

journal_title：Journal of biochemistry

authors： Tada M,Takahashi S,Miyano M,Miyake Y

更新日期：1992-08-01 00:00:00

abstract：:The nucleotide sequence of 5S rRNA from the fission yeast, S. pombe, has been established by post labeling procedures combined with cataloging RNase T1- and A-oligonucleotides derived from unlabeled 5S rRNA. The sequence consists of 119 nucleotides without a modified base and shows more dissimilarities (at 38 position...

journal_title：Journal of biochemistry

authors： Komiya H,Miyazaki M,Takemura S

更新日期：1981-05-01 00:00:00

abstract：:Incubation of quiescent cultures of Swiss 3T3 cells with epidermal growth factor (EGF) caused an increase in c-myc mRNA. Under these conditions, EGF did not induce phosphoinositide turnover, formation of diacylglycerol, formation of inositol tris-, bis-, and monophosphates, protein kinase C activation, or Ca2+ mobiliz...

journal_title：Journal of biochemistry

authors： Tsuda T,Hamamori Y,Fukumoto Y,Kaibuchi K,Takai Y

更新日期：1986-12-01 00:00:00

abstract：:It is well established that promyelocytic leukaemia nuclear bodies (PML NBs) play important roles in DNA damage responses (DDR). After irradiation, PML NBs dynamically recruit or release important proteins involved in cell-cycle regulation, DNA repair and apoptosis. As PML protein is the key molecule of PML NBs' dynam...

journal_title：Journal of biochemistry

authors： Liu J,Song Y,Tian B,Qian J,Dong Y,Liu J,Liu B,Sun Z

更新日期：2010-12-01 00:00:00

abstract：:As a first step to catalogue mRNAs present in mouse embryonal carcinoma F9 cells, 879 clones corresponding to low-abundance mRNAs were selected from among 2,896 randomly picked up clones of undifferentiated F9 cDNA libraries, using DNA probes complementary to poly(A)+RNAs prepared from undifferentiated F9 cells and to...

journal_title：Journal of biochemistry

authors： Nishiguchi S,Joh T,Horie K,Zou Z,Yasunaga T,Shimada K

更新日期：1994-07-01 00:00:00

abstract：:Nestin is an intermediate filament protein mainly expressed in muscle and neural progenitors. Recently, we reported that nestin is expressed in rat vascular smooth muscle cells (VSMCs), disappears after serum-deprivation and then is re-expressed again following EGF stimulation. As the function of nestin in VSMCs remai...

journal_title：Journal of biochemistry

authors： Huang YL,Wu CM,Shi GY,Wu GC,Lee H,Jiang MJ,Wu HL,Yang HY

更新日期：2009-09-01 00:00:00

abstract：:Nearly full-length cDNA clones for muscle-type and non-muscle-type aldolase mRNAs were cloned from lambda gt10 cDNA libraries constructed from skeletal muscle and liver mRNAs of lamprey (Entosphenus japonicus). The cDNA-M8 has 2,240 bp carrying an open reading frame of 1,089 bp which encodes 362 amino acids without th...

journal_title：Journal of biochemistry

authors： Zhang R,Yatsuki H,Kusakabe T,Iwabe N,Miyata T,Imai T,Yoshida M,Hori K

更新日期：1995-03-01 00:00:00

abstract：:Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

journal_title：Journal of biochemistry

authors： Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

更新日期：2010-04-01 00:00:00

abstract：:Purothionin isolated from commercial wheat flour contained several components and two of them (A-I and A-II) were isolated in pure form by CM-52 column chromatography. Each component contained 45 amino acid residues with a 4 disulfide bonds. Purothionin A-II was digested with trypsin and thermolysin to isolate cystine...

journal_title：Journal of biochemistry

authors： Hase T,Matsubara H,Yoshizumi H

更新日期：1978-06-01 00:00:00

abstract：:Spontaneous insertion of membrane proteins into liposomes formed from Escherichia coli polar phospholipids is blocked by diacylglycerol (DAG) at a physiological level. We found that cholesterol also blocks this spontaneous insertion, although a much larger amount is necessary for sufficient blockage. Reversely, sphing...

journal_title：Journal of biochemistry

authors： Nakamura S,Suzuki S,Saito H,Nishiyama KI

更新日期：2018-04-01 00:00:00

abstract：:A radioimmunoassay for chicken calcitonin in chicken ultimobranchial glands was established utilizing a rabbit antiserum against eel calcitonin. This assay method, which is about 100 times as sensitive as the usual bioassay for hypocalcemic activity, was used for monitoring chicken calcitonin during its purification. ...

journal_title：Journal of biochemistry

authors： Homma T,Watanabe M,Hirose S,Kanai A,Kangawa K,Matsuo H

更新日期：1986-08-01 00:00:00

abstract：:A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which...

journal_title：Journal of biochemistry

authors： Umeki N,Mitsui T,Koike Y,Maruta S

更新日期：2006-05-01 00:00:00

abstract：:Studies on the solution structure dynamics of RNA/DNA are becoming crucially important. The phenomena of SSCP (single-strand conformation polymorphism), small RNA dynamics in a cell, and others can be related to the conformational changes of single-stranded (ss) RNAs/DNAs in solution. However, little is known about th...

journal_title：Journal of biochemistry

authors： Biyani M,Nishigaki K

更新日期：2005-10-01 00:00:00

abstract：:MicroRNAs (miRNAs) serve as key regulators in human disorders. Previous research reported that miR-211-5p is down-regulated in osteoarthritis (OA) and that Fibulin-4 inhibits chondrocyte differentiation. However, the role of miR-211-5p in the development of OA has not been clarified, and its downstream target has not ...

journal_title：Journal of biochemistry

authors： Liu H,Luo J

更新日期：2019-12-01 00:00:00

abstract：:The cDNA of a novel protein, which contains the association domain of alpha isoform of calmodulin-dependent protein kinase II (CaM-kinase II alpha), was cloned from rat skeletal muscle. This protein, called alpha KAP, consisted of 200 amino acid residues with a molecular weight of 22,583. alpha KAP has a highly hydrop...

journal_title：Journal of biochemistry

authors： Sugai R,Takeuchi M,Okuno S,Fujisawa H

更新日期：1996-10-01 00:00:00

abstract：:Polysialoglycoprotein (PSGP) is a major cortical alveolus glycoprotein of rainbow trout eggs that is characterized by the attachment of a polysialic acid structure to its O-glycan chains. It has been demonstrated that the polysialic acid structure is synthesized by at least three sialyltransferases mostly localized in...

journal_title：Journal of biochemistry

authors： Asahina S,Sato C,Kitajima K

更新日期：2004-08-01 00:00:00

abstract：:The 1H-NMR spectra of eight unsaturated disaccharides obtained by bacterial eliminase digestion of chondroitin sulfate and of heparan sulfate/heparin were recorded in order to construct an NMR data base of sulfated oligosaccharides and to investigate the effects of sulfation on the proton chemical shifts. These shifts...

journal_title：Journal of biochemistry

authors： Yamada S,Yoshida K,Sugiura M,Sugahara K

更新日期：1992-10-01 00:00:00

abstract：:The effects of freezing on Alcaligenes sp. nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, EC 1.7.2.1] dissolved in sodium phosphate (pH 7.2) were investigated. The nitrite reductase was gradually activated with time in the frozen state, resulting in an increase in its activity of 2.5-4.5 times. The...

journal_title：Journal of biochemistry

authors： Masuko M,Iwasaki H,Sakurai T,Suzuki S,Nakahara A

更新日期：1985-11-01 00:00:00

abstract：:An inhibitor of blood coagulation, a new protein with an apparent molecular weight of 34,000 and an isoelectric point of 4.9, was purified from human placental tissue by EDTA extraction. Five cDNA clones were isolated from the human placental lambda gt11 cDNA library using the mouse monoclonal antibody raised against ...

journal_title：Journal of biochemistry

authors： Iwasaki A,Suda M,Nakao H,Nagoya T,Saino Y,Arai K,Mizoguchi T,Sato F,Yoshizaki H,Hirata M

更新日期：1987-11-01 00:00:00

abstract：:The three-dimensional structure of cytochrome c-553 isolated from sulfate-reducing bacterium, Desulfovibrio vulgaris Miyazaki F strain, has been determined by the multi-wavelength anomalous dispersion technique with use of synchrotron radiation. The result shows that bacterial S-class cytochromes c have a variety of f...

journal_title：Journal of biochemistry

authors： Nakagawa A,Higuchi Y,Yasuoka N,Katsube Y,Yagi T

更新日期：1990-11-01 00:00:00

abstract：:Sialoglycoprotein which exhibits inhibitory activity for hemagglutination by Hemagglutinating Virus of Japan (HVJ, Sendai virus) was isolated from the membrane of bovine erythrocytes. Purification steps for this sialoglycoprotein included extraction with lithium diiodosalicylate, phenol partition, precipitation with e...

journal_title：Journal of biochemistry

authors： Suzuki Y,Suzuki T,Matsumoto M

更新日期：1983-06-01 00:00:00

abstract：:Ca2+-binding of S-100 protein was studied using a Ca2+ electrode at pH 6.80. In the presence of 0.1 M KCl and 10 mM MgCl2 (ionic strength 0.13), Ca2+-binding to S-100 protein occurred in three steps with positive cooperativity. The numbers of bound Ca2+ ions in the three steps were 2, 2, and 4. The Ca2+-binding consta...

journal_title：Journal of biochemistry

authors： Matsuda S

更新日期：1988-12-01 00:00:00

abstract：:The enthalpies of transfer of several amino acids and diglycine from water to aqueous polyols (glycerol, xylitol, sorbitol, and inositol) have been calorimetrically determined in order to clarify the mechanism of polyol-induced stabilization of proteins. The obtained enthalpy data were combined with the data on free e...

journal_title：Journal of biochemistry

authors： Gekko K

更新日期：1981-12-01 00:00:00