abstract：:Cancer immunotherapy is a promising intervention to fight against this global health problem. In particular targeting immune checkpoints, such as cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and programmed-death protein 1 (PD-1), by specific monoclonal antibodies is a current treatment for many malignances. A ...

journal_title：Journal of biotechnology

authors： Yiemchavee S,Wong-Arce A,Romero-Maldonado A,Shanmugaraj B,Monsivais-Urenda A,Phoolcharoen W,Rosales-Mendoza S

更新日期：2021-01-21 00:00:00

abstract：:A common approach for analyzing large-scale molecular data is to cluster objects sharing similar characteristics. This assumes that genes with highly similar expression profiles are likely participating in a common molecular process. Biological systems are extremely complex and challenging to understand, with proteins...

journal_title：Journal of biotechnology

authors： Ulfenborg B,Karlsson A,Riveiro M,Andersson CX,Sartipy P,Synnergren J

更新日期：2021-01-20 00:00:00

abstract：:The present study aims to valorize chestnut burrs, an important lignocellulosic waste, through a biorefinery concept. A solid residue rich in glucan (41.36 ± 0.59 %) and lignin (39.06 ± 0.01 %) obtained from a previous process of pre-hydrolysis was subjected to four treatments with water or NaOH to enhance enzymatic h...

journal_title：Journal of biotechnology

authors： Costa-Trigo I,Paz A,Otero-Penedo P,Outeiriño D,Pérez Guerra N,Domínguez JM

更新日期：2021-01-18 00:00:00

abstract：:Deracemization of D,L-phosphinothricin (D,L-PPT) is one of the most promising routes for preparation of optically pure L-PPT. In this work, an efficient multi-enzyme redox cascade was developed for deracemization ofPPT, which includes oxidative reaction and reductive reaction. The oxidative reaction catalyzing oxidati...

journal_title：Journal of biotechnology

authors： Cao CH,Gong H,Dong Y,Li JM,Cheng F,Xue YP,Zheng YG

更新日期：2021-01-10 00:00:00

abstract：:Flavonoids have notable biological activities and have been widely used in the medicinal and chemical industries. However, single-copy integration of heterologous pathway genes limits the production of flavonoids. In this work, we designed and constructed single-step integration of multiple flavonoid (2S)-naringenin b...

journal_title：Journal of biotechnology

authors： Li H,Gao S,Zhang S,Zeng W,Zhou J

更新日期：2021-01-10 00:00:00

abstract：:(S)-2-chlorophenylglycine methyl ester ((S)-1) is a key chiral building block of clopidogrel, which is a widely administered antiaggregatory and antithrombotic drug. Herein, Protease 6SD was covalently immobilized on multi-walled carbon nanotubes (MWCNT), and the as-prepared immobilizate P-6SD@NH2-MWCNT was applied in...

journal_title：Journal of biotechnology

authors： Weng CY,Wang DN,Ban SY,Zhai QY,Hu XY,Cheng F,Wang YJ,Zheng YG

更新日期：2021-01-10 00:00:00

abstract：:The asymmetric reduction of ketones is a frequently used synthesis route towards chiral alcohols. Amongst available chemo- and biocatalysts the latter stand out in terms of product enantiopurity. Their application is, however, restricted by low reaction output, often rooted in limited enzyme stability under operationa...

journal_title：Journal of biotechnology

authors： Rapp C,Nidetzky B,Kratzer R

更新日期：2021-01-10 00:00:00

abstract：:Unicellular green algae, a promising source for renewable biofuels, produce lipid-rich biomass from light and CO2. Productivity in photo-bioreactors is affected by inhomogeneous light distribution from high cell pigment causing heat dissipation of light energy absorbed in excess and shading of the deep layers. Contras...

journal_title：Journal of biotechnology

authors： Barera S,Dall'Osto L,Bassi R

更新日期：2021-01-09 00:00:00

abstract：:Soil salinity is one of the major environmental factors, influencing agricultural productivity of crops. As a non-edible and ideal oilseed crop, castor (Ricinus communis L.) has great industrial value in biofuel, but molecular mechanisms of salt stress regulation are still unknown. In this study, the differentially ex...

journal_title：Journal of biotechnology

authors： Lei P,Liu Z,Hu Y,Kim H,Liu S,Liu J,Xu L,Li J,Zhao Y,Yu Z,Qu Y,Huang F,Meng F

更新日期：2021-01-06 00:00:00

abstract：:Palladium (Pd) is commonly used as a catalyst for automobiles and electronic devices, and a reliable source of Pd is required for continued commercial applications. Biomineralization has attracted attention as an inexpensive and eco-friendly recycling approach for a continued supply of Pd. Escherichia coli is one of t...

journal_title：Journal of biotechnology

authors： Matsumoto T,Kamino M,Yamada R,Konishi Y,Ogino H

更新日期：2020-12-20 00:00:00

abstract：:ACC deaminase producing Plant growth promoting rhizobacteria (PGPR) offers a great promise for ameliorating the negative impacts of salinity stress manifested on plants. In this context, 28 rhizospheric bacteria associated with ACC deaminase potential (198-1069 nmol α-ketobutyrate mg protein-1 h-1) were isolated from ...

journal_title：Journal of biotechnology

authors： Pandey S,Gupta S

更新日期：2020-12-20 00:00:00

abstract：:Indigo is an insoluble blue dye, which generates serious pollution in its production process. Increasing focus has come to the biosynthesis of indigo that are more environment-preserved and high-efficient. Hence, this study was designed to explore the specific role of various deep eutectic solvents (DESs) on cytochrom...

journal_title：Journal of biotechnology

authors： Sun H,Xin R,Qu D,Yao F

更新日期：2020-11-10 00:00:00

abstract：:Particulate methane monooxygenase (pMMO) is a membrane protein embedded in the intracytoplasmic membrane of methane-oxidizing bacteria. Structural analysis of pMMO showed the existence of a hydrophilic region exposed outside of the bacterial membrane. To obtain information regarding the role of this hydrophilic region...

journal_title：Journal of biotechnology

authors： Miyaji A,Satou K,Baba T

更新日期：2020-11-10 00:00:00

abstract：:The utilisation of urban organic waste as feedstock for polyhydroxyalkanoates (PHA) production is growing since it allows to solve the main concerns about their disposal and simultaneously to recover added-value products. A pilot scale platform has been designed for this purpose. The VFA-rich fermentation liquid comin...

journal_title：Journal of biotechnology

authors： Valentino F,Lorini L,Gottardo M,Pavan P,Majone M

更新日期：2020-11-10 00:00:00

abstract：:The adsorption behaviors of two glucanases, TvEG and TrCel7A, on three lignins were investigated. Three lignins were isolated from raw aspen and its pretreated solid residue. The isolated lignins were labeled as Asp-MWL, DA-MWL (pretreated by dilute acid), and GL-MWL (pretreated by green liquor), respectively. The sur...

journal_title：Journal of biotechnology

authors： Zhang Y,Jiang X,Wan S,Wu W,Wu S,Jin Y

更新日期：2020-11-10 00:00:00

abstract：:Nanobiotechnological improvements defined on the utilization of biological materials and principles have enormously partaken to revolutionize physical, chemical, and biological sciences. However, the exploration of plant nanobiotechnology is still in its outset. The search for novel tools to monitor plant biomolecules...

journal_title：Journal of biotechnology

authors： Ambrin G,Kausar H,Ahmad A

更新日期：2020-11-10 00:00:00

abstract：:Genome shuffling is an efficient way to pool advantageous traits during positive selections of industrial microorganisms. In this study, for the first time, the effectiveness of genome shuffling to improve yielding of heterologous proteins was investigated in Kluyveromyces marxianus (KM), a promising yeast host. After...

journal_title：Journal of biotechnology

authors： Wu L,Wang M,Zha G,Zhou J,Yu Y,Lu H

更新日期：2020-08-20 00:00:00

abstract：:siRNA based therapeutics have become the next frontier in molecular medicine. Though exosomes emerge as a promising drug delivery vehicle for siRNAs, significant hurdle remains in finding safe and effective loading methods. Traditional methods of loading exogenous siRNAs in exosomes are marked by certain limitations l...

journal_title：Journal of biotechnology

authors： Shandilya S,Rani P,Onteru SK,Singh D

更新日期：2020-07-20 00:00:00

abstract：:Norovirus infections cause severe gastroenteritis in millions of people every year. Infection requires the recognition of histo-blood group antigens (HBGAs), but such interactions can be inhibited by human milk oligosaccharides (HMOs), which act as structurally-similar decoys. HMO supplements could help to prevent nor...

journal_title：Journal of biotechnology

authors： Derya SM,Spiegel H,Hanisch FG,Morozov V,Schroten H,Jennewein S,Parschat K

更新日期：2020-07-20 00:00:00

abstract：:Bacterial pigments are potential substitute of chemical photosensitizer for dye-sensitized solar cell (DSSC) due to its non-toxic property and cost-effective production from microbial fermentation. Serratia nematodiphila YO1 was isolated from waterfall in Malaysia and identified using 16S ribosomal RNA. Characterizati...

journal_title：Journal of biotechnology

authors： Abdul Manas NH,Chong LY,Tesfamariam YM,Zulkharnain A,Mahmud H,Abang Mahmod DS,Mohamad Fuzi SFZ,Wan Azelee NI

更新日期：2020-06-20 00:00:00

abstract：:The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacter...

journal_title：Journal of biotechnology

authors： Tan IKP,Foong CP,Tan HT,Lim H,Zain NA,Tan YC,Hoh CC,Sudesh K

更新日期：2020-04-10 00:00:00

abstract：:Ebola virus disease outbreaks have repeatedly occurred on the African continent over the last decades, with more serious outbreaks in recent years. Being highly transmissible and associated to high fatality rates, it constitutes a serious threat to public health. Vaccination, however, may allow for efficient control o...

journal_title：Journal of biotechnology

authors： Kiesslich S,Vila-Chã Losa JP,Gélinas JF,Kamen AA

更新日期：2020-02-20 00:00:00

abstract：:The inflammation of chronic wounds generally causes delaying their healing process. The present work aims to formulate a wound dressing polyelectrolyte membrane based on chitosan (Ch) and sodium hyaluronate (HA) loaded with glutathione (GSH). The membrane types (Ch/HA and Ch/HA/GSH) were examined by Fourier transform ...

journal_title：Journal of biotechnology

authors： Tamer TM,Hassan MA,Valachová K,Omer AM,El-Shafeey MEA,Mohy Eldin MS,Šoltés L

更新日期：2020-02-20 00:00:00

abstract：:The study was conducted to investigate the effects of foliar-intervention of nano-TiO2 on Cd toxicity in cowpea plants. Cowpea plants were exposed to Cd toxicity at 10 mg/kg soil for 21 days and afterwards, subjected to six episodes of foliar application of nano-TiO2 intervention. Results showed that foliar-applied na...

journal_title：Journal of biotechnology

authors： Ogunkunle CO,Odulaja DA,Akande FO,Varun M,Vishwakarma V,Fatoba PO

更新日期：2020-02-20 00:00:00

abstract：:Ascomycete fungi Cordyceps are widely used in traditional Chinese medicine, and numerous investigations have been carried out to uncover their biological activities. However, primary researches on the physiological effects of Cordyceps were committed using crude extracts. At present, there are only a few compounds whi...

journal_title：Journal of biotechnology

authors： Wang X,Gao YL,Zhang ML,Zhang HD,Huang JZ,Li L

更新日期：2020-02-10 00:00:00

abstract：:S-adenosyl-l-methionine (SAM) has been attracting increasing attention because of its significance in the pharmaceutical industry; however, the high cost of this compound limits its application. Tofu yellow serofluid exhibits high nutritional value and is not costly; therefore, it can be utilized as a substrate in the...

journal_title：Journal of biotechnology

authors： Li G,Li H,Tan Y,Hao N,Yang X,Chen K,Ouyang P

更新日期：2020-02-10 00:00:00

abstract：:To meet the food demand of growing population, agricultural productivity needs to be increased by employing safe strategies without harming ecosystem. Silver nanoparticle (AgNP) using a green approach has become a promising substitute to the synthetic pesticides to overcome pest menace. In this study, AgNPs were synth...

journal_title：Journal of biotechnology

authors： Vanti GL,Kurjogi M,Basavesha KN,Teradal NL,Masaphy S,Nargund VB

更新日期：2020-02-10 00:00:00

abstract：:Amatoxins are ribosomally synthesized and post-translationally modified peptides (RiPPs) found in poisonous mushrooms. These cyclic peptides are potent inhibitors of RNA polymerase II transcriptional activity. Though the macrocyclization of amatoxin is extensively studied, little is known about its subsequent post-tra...

journal_title：Journal of biotechnology

authors： Pathiraja D,Byun J,Lee S,Choi IG

更新日期：2020-01-20 00:00:00

abstract：:Zeolitic imidazole frameworks (ZIFs) with tunable pore sizes and high surface areas have recently used as an effective support for immobilizing enzymes. However, the instability in the aqueous acidic environment has limited their practical applications in some cases. In this work, we develop a novel catalase/ZIFs comp...

journal_title：Journal of biotechnology

authors： Feng Y,Zhong L,Hou Y,Jia S,Cui J

更新日期：2019-12-20 00:00:00

abstract：:Rhizomucor miehei lipase (RML), a GRAS catalyst with wide applications, was overexpressed in Yarrowia lipolytica, also a GRAS unconventional yeast, via a combined strategy, optimization for promoter, gene dosage and fermentation process. The lipase activity of the recombinant strain was first increased from 19.5 to 26...

journal_title：Journal of biotechnology

authors： Zhou Q,Jiao L,Qiao Y,Wang Y,Xu L,Yan J,Yan Y

更新日期：2019-12-20 00:00:00

abstract：:Chimeric virus-like particles (VLP) are known as promising tools in the development of safe and effective subunit vaccines. Recently, a technology platform to produce VLP based on the small surface protein (dS) of the duck hepatitis B virus was established. In this study, chimeric VLP were investigated displaying the ...

journal_title：Journal of biotechnology

authors： Wetzel D,Barbian A,Jenzelewski V,Schembecker G,Merz J,Piontek M

更新日期：2019-12-20 00:00:00

abstract：:The valorization of a solid carob waste from the Lebanese industry was investigated by optimizing the production of lactic acid using immobilized Lactobacillus rhamnosus in alginate beads and response surface methodology. The results showed that pH and alginate concentration had a significant effect on the production ...

journal_title：Journal of biotechnology

authors： Bahry H,Abdalla R,Pons A,Taha S,Vial C

更新日期：2019-12-20 00:00:00

abstract：:Secreted recombinant proteins can aggregate during cell culture. We studied a poorly-behaved bispecific scaffold that increasingly aggregated (up to 62% high molecular weight species, HMW) as a function of culture time in a fed-batch and intensified cell culture processes. We identified that protein aggregates increas...

journal_title：Journal of biotechnology

authors： Gomez N,Barkhordarian H,Lull J,Huh J,GhattyVenkataKrishna P,Zhang X

更新日期：2019-10-10 00:00:00

abstract：:The Semliki Forest virus (SFV) viral vector has been widely used for transient protein expression. This study aimed to analyze comprehensively the capacity of SFV vector to express rabies lyssavirus glycoprotein (RVGP) in mammalian cells. The assessed parameters were transfection strategy, multiplicity of infection (M...

journal_title：Journal of biotechnology

authors： de Rezende AG,Fernández Núñez EG,Astray RM,Puglia ALP,Pereira CA,Jorge SAC

更新日期：2019-10-10 00:00:00

abstract：:Many recent epigenetic studies utilize the advantages of CRISPR/dCas9 based tools in linking certain epigenetic modification with gene expression regulation. Various multifactorial diseases often contain changed epigenetic signatures at many loci, so tools for simultaneously targeting different loci would significantl...

journal_title：Journal of biotechnology

authors： Josipović G,Zoldoš V,Vojta A

更新日期：2019-08-10 00:00:00

abstract：:(+)-Nootkatone is a natural ingredient that occurs in grapefruit and certain other plants and is responsible for the characteristic smell of grapefruit. Due to its versatile applications in the flavor and fragrance industry as well as its application in some medical uses it recruits the interests of academic research ...

journal_title：Journal of biotechnology

authors： Milhim M,Hartz P,Gerber A,Bernhardt R

更新日期：2019-08-10 00:00:00

abstract：:Increasing levels of antibiotic resistance in pathogens, including Staphylococcus aureus, remains a serious problem for public health, leading to the need for better alternative antimicrobial strategies. The antimicrobial proteins produced by Lactobacillus plantarum USM8613 attributed to its anti-staphylococcal activi...

journal_title：Journal of biotechnology

authors： Ong JS,Taylor TD,Wong CB,Khoo BY,Sasidharan S,Choi SB,Ohno H,Liong MT

更新日期：2019-07-20 00:00:00

abstract：:While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developi...

journal_title：Journal of biotechnology

authors： Keresztessy Z,Erdos E,Ozgyin L,Kádas J,Horváth J,Zahuczky G,Balint BL

更新日期：2019-07-20 00:00:00

abstract：:In this study, a commercial lipase derived from Candida cylindracea was chemically modified with dextran by conjugating ε-amine in the lysine residue with the carbonyl residue in oxidized dextran using the borane-pyridine complex as a reducing agent to increase the hydrophilicity of the microenvironment around the lip...

journal_title：Journal of biotechnology

authors： Kajiwara S,Komatsu K,Yamada R,Matsumoto T,Yasuda M,Ogino H

更新日期：2019-04-20 00:00:00

abstract：:The aim of this work was the production of bioactive metabolites by submerged fermentation from the fungus Diaporthe schini, followed by their extraction, separation and characterization. Different solvents (methanol, dichloromethane and hexane) were used for the extraction of metabolites from the fermentation broth a...

journal_title：Journal of biotechnology

authors： Dos Reis CM,da Rosa BV,da Rosa GP,do Carmo G,Morandini LMB,Ugalde GA,Kuhn KR,Morel AF,Jahn SL,Kuhn RC

更新日期：2019-03-20 00:00:00