Abstract：
:Increasing levels of antibiotic resistance in pathogens, including Staphylococcus aureus, remains a serious problem for public health, leading to the need for better alternative antimicrobial strategies. The antimicrobial proteins produced by Lactobacillus plantarum USM8613 attributed to its anti-staphylococcal activity were identified as extracellular transglycosylase and glyceraldehyde-3-phosphate dehydrogenase (GADPH), both with different mechanisms of action. Extracellular transglycosylase, which contains a LysM domain, exerts a cell wall-mediated killing mechanism, while GADPH penetrates into S. aureus cells and subsequently induces the overexpression of autolysis regulators, resulting in S. aureus autolysis. Both extracellular transglycosylase and GADPH exert anti-inflammatory effects in S. aureus-infected HaCaT cells by reducing the expression and production of TLR-2, hBDs and various pro-inflammatory cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-8). Taken together, extracellular transglycosylase and GADPH produced by L. plantarum USM8613 could potentially be applied as an alternative therapeutic agent to treat S. aureus skin infections and promote skin health.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Ong JS,Taylor TD,Wong CB,Khoo BY,Sasidharan S,Choi SB,Ohno H,Liong MTdoi
10.1016/j.jbiotec.2019.05.006subject
Has Abstractpub_date
2019-07-20 00:00:00pages
20-31eissn
0168-1656issn
1873-4863pii
S0168-1656(19)30162-2journal_volume
300pub_type
杂志文章abstract：:Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...
journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：2014-12-20 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2007.04.018
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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更新日期：2016-03-20 00:00:00
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