当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Acid-resistant enzyme@MOF nanocomposites with mesoporous silica shells for enzymatic applications in acidic environments.

Acid-resistant enzyme@MOF nanocomposites with mesoporous silica shells for enzymatic applications in acidic environments.

Abstract：

:Zeolitic imidazole frameworks (ZIFs) with tunable pore sizes and high surface areas have recently used as an effective support for immobilizing enzymes. However, the instability in the aqueous acidic environment has limited their practical applications in some cases. In this work, we develop a novel catalase/ZIFs composite with mesoporous silica shell (mSiO2@CAT/ZIFs) via co-precipitation, and controlled self-assembly of silanes. During preparation, the cetyltrimethylammonium bromide induced the formation of the mesostructured silica layer on the outer surface of CAT/ZIFs. The resultant mSiO2@CAT/ZIFs exhibited high activity recovery (92%). Compared with the conventional CAT/ZIFs and free CAT, mSiO2@CAT/ZIFs exhibited excellent acid resistance. For example, after 30 min in acetate buffer solution (pH 3.0), the CAT/ZIFs and free CAT almost lost activity whereas the mSiO2@CAT/ZIFs still retained 35% of original activity. Meanwhile, the thermostability of the mSiO2@CAT/ZIFs was enhanced significantly compared with conventional CAT/ZIFs. In addition, the mSiO2@CAT/ZIFs displayed excellent storage stability, and retained 60% of its initial activity after 15 days storage period. Furthermore, the mSiO2@CAT/ZIFs could maintain 70% of its initial activity after 8 continuous uses, demonstrating superior reusability than the free CAT and CAT/ZIFs. These results demonstrated that the mSiO2@CAT/ZIFs are potential for practical applications even in the acidic environment.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Feng Y,Zhong L,Hou Y,Jia S,Cui J

doi

10.1016/j.jbiotec.2019.09.010

subject

Has Abstract

pub_date

2019-12-20 00:00:00

pages

54-61

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(19)30858-2

journal_volume

306

pub_type

杂志文章

相关文献

JOURNAL OF BIOTECHNOLOGY文献大全
  • The GlxR regulon of the amino acid producer Corynebacterium glutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model.

    abstract：:Corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the Corynebacterineae. The glxR gene of C. glutamicum ATCC 13032 encodes a DNA binding transcription factor of the Crp-Fnr protein family. Available data indicated a prominent role of GlxR in the transcript...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2009.08.005

    authors： Kohl TA,Tauch A

    更新日期：2009-09-25 00:00:00

  • A novel promoterless gene targeting vector to efficiently disrupt PRNP gene in cattle.

    abstract：:The PRNP gene encodes a cellular protein named prion, whose misfolded form has been implicated in a number of neuropathic diseases in mammals such as the Bovine Spongiform Encephalopathy (BSE) in cattle. BSE has brought devastating impact on the world economy and human health. Recently, several groups have performed t...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.10.018

    authors： Wang S,Zhang K,Ding F,Zhao R,Li S,Li R,Xu L,Song C,Dai Y,Li N

    更新日期：2013-02-20 00:00:00

  • Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris.

    abstract：:The methylotropic yeast Pichia pastoris has demonstrated its suitability for large-scale production of recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins when compared with Escherichia coli. In this work, P. pastoris has bee...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.04.024

    authors： de la Cruz S,Alcocer M,Madrid R,García A,Martín R,González I,García T

    更新日期：2016-06-10 00:00:00

  • N-linked glycosylation influences on the catalytic and biochemical properties of Penicillium purpurogenum β-d-glucuronidase.

    abstract：:To study the influence of N-linked carbohydrate moiety on the catalytic and biochemical properties of glycosylated enzyme, a recombinant β-d-glucuronidase (PGUS-P) from Penicillium purpurogenum as a model glycoprotein, was deglycosylated with peptide-N-glycosidase F (PNGase-F) under native conditions. The enzymatic de...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.12.017

    authors： Zou S,Xie L,Liu Y,Kaleem I,Zhang G,Li C

    更新日期：2012-02-10 00:00:00

  • Chemostat studies of bacteriophage M13 infected Escherichia coli JM109 for continuous ssDNA production.

    abstract：:Steady state studies in a chemostat enable the control of microbial growth rate at defined reaction conditions. The effects of bacteriophage M13 infection on maximum growth rate of Escherichia coli JM109 were studied in parallel operated chemostats on a milliliter-scale to analyze the steady state kinetics of phage pr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.06.409

    authors： Kick B,Behler KL,Severin TS,Weuster-Botz D

    更新日期：2017-09-20 00:00:00

  • Towards the computational design and engineering of enzyme enantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans.

    abstract：:In our previous work, a NAD(H)-dependent carbonyl reductase (GoCR) was identified from Gluconobacter oxydans, which showed moderate to high enantiospecificity for the reduction of different kinds of prochiral ketones. In the present study, the crystal structure of GoCR was determined at 1.65Å resolution, and a computa...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.11.003

    authors： Deng J,Yao Z,Chen K,Yuan YA,Lin J,Wei D

    更新日期：2016-01-10 00:00:00

  • Plasmid instability kinetics in continuous culture of a recombinant Saccharomyces cerevisiae in airlift bioreactor.

    abstract：:Plasmid instability of a recombinant Saccharomyces cerevisiae C468/pGAC9 (ATCC 20690) was examined during continuous culture in a nonselective medium in an airlift bioreactor. The recombinant strain contained a 2-micron based shuttle vector pGAC9 and expresses Aspergillus awatnori glucoamylase gene under the control o...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(97)00057-6

    authors： Zhang Z,Scharer JM,Moo-Young M

    更新日期：1997-05-23 00:00:00

  • Fast and efficient protein purification using membrane adsorber systems.

    abstract：:The purification of proteins from complex cell culture samples is an essential step in proteomic research. Traditional chromatographic methods often require several steps resulting in time consuming and costly procedures. In contrast, protein purification via membrane adsorbers offers the advantage of fast and gentle ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.07.023

    authors： Suck K,Walter J,Menzel F,Tappe A,Kasper C,Naumann C,Zeidler R,Scheper T

    更新日期：2006-02-10 00:00:00

  • Mammalian cell culture scale-up and fed-batch control using automated flow cytometry.

    abstract：:Detailed knowledge of mammalian cell culture proliferation kinetics is important to determine fed-batch strategies for industrial bioreactor operations. In particular, predicting the end of exponential proliferation in batch culture is a critical process parameter during culture scale-up. Using automated flow cytometr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2008.03.019

    authors： Sitton G,Srienc F

    更新日期：2008-06-01 00:00:00

  • Immobilization of Aspergillus terreus lipase in self-assembled hollow nanospheres for enantioselective hydrolysis of ketoprofen vinyl ester.

    abstract：:The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate-graft-poly(ethylene glycol)/α-cyclodextrins (Alg-g-PEG/α-CD) spheres as enzyme immobilization carriers. The morphology and size o...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.11.032

    authors： Hu C,Wang N,Zhang W,Zhang S,Meng Y,Yu X

    更新日期：2015-01-20 00:00:00

  • A new molecular approach based on qPCR for the quantification of fecal bacteria in contaminated marine sediments.

    abstract：:Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.07.033

    authors： Luna GM,Dell'Anno A,Pietrangeli B,Danovaro R

    更新日期：2012-02-20 00:00:00

  • Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099.

    abstract：:Two extra-cellular endoxylanases (Xyl Ia and Ib) were purified to homogeneity from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Xyl Ia and Ib, having a molecular mass of approximately 53 kDa and pI of 5.2 and 4.8, respectively, were optimally active at 75 degrees C and at pH 6.0. They were st...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2003.12.010

    authors： Chadha BS,Ajay BK,Mellon F,Bhat MK

    更新日期：2004-04-29 00:00:00

  • Development of an antibody control system using phage display.

    abstract：:While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2019.05.009

    authors： Keresztessy Z,Erdos E,Ozgyin L,Kádas J,Horváth J,Zahuczky G,Balint BL

    更新日期：2019-07-20 00:00:00

  • Novel microbial epoxide hydrolases for biohydrolysis of glycidyl derivatives.

    abstract：:Microbial isolates from biofilters and petroleum-polluted bioremediation sites were screened for the presence of enantioselective epoxide hydrolases active towards tert-butyl glycidyl ether, benzyl glycidyl ether, and allyl glycidyl ether. Out of 270 isolated strains, which comprised bacteria, yeasts, and filamentous ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.06.011

    authors： Kotik M,Brichac J,Kyslík P

    更新日期：2005-12-06 00:00:00

  • Expression levels of antimicrobial peptide tachyplesin I in transgenic Ornithogalum lines affect the resistance to Pectobacterium infection.

    abstract：:The genus Ornithogalum includes several ornamental species that suffer substantial losses from bacterial soft rot caused by Pectobacteria. The absence of effective control measures for use against soft rot bacteria led to the initiation of a project in which a small antimicrobial peptide from an Asian horseshoe crab, ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.09.008

    authors： Lipsky A,Joshi JR,Carmi N,Yedidia I

    更新日期：2016-11-20 00:00:00

  • Enzymatic peptide synthesis in organic media: a comparative study of water-miscible and water-immiscible solvent systems.

    abstract：:Peptide synthesis was carried out in a variety of organic solvents with low contents of water. The enzyme was deposited on the support material, celite, from an aqueous buffer solution. After evaporation of the water the biocatalyst was suspended in the reaction mixtures. The chymotrypsin-catalyzed reaction between Z-...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(90)90090-x

    authors： Clapés P,Adlercreutz P,Mattiasson B

    更新日期：1990-09-01 00:00:00

  • Targeted delivery of calreticulin to ED-A fibronectin leads to tumor-growth retardation.

    abstract：:We report the design and characterization of novel fusion proteins, consisting of the F8 antibody and of murine calreticulin (Calr). The F8 antibody recognizes the alternatively-spliced ED-A domain of fibronectin, an extracellular matrix component found in most tumor types, while calreticulin has previously been descr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2018.12.007

    authors： Ziffels B,Grötsch A,Al-Bayati L,Neri D

    更新日期：2019-01-20 00:00:00

  • Correct in vivo processing of a chimeric ubiquitin-proapolipoprotein A-I fusion protein in baculovirus-infected insect cells.

    abstract：:The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product was purified and it...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(94)90118-x

    authors： Moguilevsky N,Guillaume JP,Varsalona F,Bulinckx M,Bollen A

    更新日期：1994-01-15 00:00:00

  • Design of nanoemulsion-based delivery systems of natural antimicrobials: effect of the emulsifier.

    abstract：:This work aims at investigating the effect of the nanoemulsion delivery systems on the antimicrobial activity of different essential oil components. Carvacrol, limonene and cinnamaldehyde were encapsulated in the sunflower oil droplets of nanoemulsions prepared by high pressure homogenization and stabilized by differe...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.07.001

    authors： Donsì F,Annunziata M,Vincensi M,Ferrari G

    更新日期：2012-06-30 00:00:00

  • Enrichment strategies for siRNA-transfected cells in an untransfected background.

    abstract：:Gene silencing experiments in difficult-to-transfect cells are often hampered by the presence of a background of untransfected cells. We present proof-of-concept data from two different strategies for enrichment of siRNA-transfected cells. In the first approach, a heterologous surface antigen is expressed from a plasm...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.04.018

    authors： Narz F,Hübner S,Magyar S,Bielke W,Weber M,Dennig J

    更新日期：2007-06-30 00:00:00

  • Synthesis of polyhydroxyalkanoates from different short-chain fatty acids by mixed cultures submitted to aerobic dynamic feeding.

    abstract：:The production of polyhydroxyalkanoates from acetate and propionate by two mixed cultures well adapted to each of these substrates was evaluated. Sludge fed with acetate (A), produced a homopolymer of hydroxybutyrate (HB), whereas sludge fed with propionate (P) produced a copolymer of HB and HV (hydoxyvalerate). Switc...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.09.006

    authors： Lemos PC,Serafim LS,Reis MA

    更新日期：2006-03-23 00:00:00

  • Back propagation neural network (BPNN) prediction model and control strategies of methanogen phase reactor treating traditional Chinese medicine wastewater (TCMW).

    abstract：:Based on the prototype experiment of treating herb wastewater by Up flow Anaerobic Sludge Bed and Anaerobic Filter reactor (UASBAF), an artificial neural network (ANN) model which adopts a back propagation algorithm with momentum and adaptive learning rate was established. And the effect of each parameter to the perfo...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2009.08.014

    authors： Shi Y,Zhao XT,Zhang YM,Ren NQ

    更新日期：2009-10-12 00:00:00

  • Influence of tryptic hydrolysis on the enzymatic function of the membrane-bound form of particulate methane monooxygenase from Methylosinus trichosporium OB3b.

    abstract：:Particulate methane monooxygenase (pMMO) is a membrane protein embedded in the intracytoplasmic membrane of methane-oxidizing bacteria. Structural analysis of pMMO showed the existence of a hydrophilic region exposed outside of the bacterial membrane. To obtain information regarding the role of this hydrophilic region...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2020.08.006

    authors： Miyaji A,Satou K,Baba T

    更新日期：2020-11-10 00:00:00

  • Wafer-scale nanowell array patterning based electrochemical impedimetric immunosensor.

    abstract：:We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.08.029

    authors： Lee J,Cho S,Lee J,Ryu H,Park J,Lim S,Oh B,Lee C,Huang W,Busnaina A,Lee H

    更新日期：2013-12-01 00:00:00

  • Rapid quantification of murine endothelin-1 and vasoactive intestinal contractor gene expression levels by a real-time PCR system.

    abstract：:A rapid quantitative analysis method for murine endothelin-1 (ET-1) and vasoactive intestinal contractor (VIC) gene expression levels was established using a real-time polymerase chain reaction (PCR). We designed primer pairs and TaqMan probes specific for murine prepro-ET-1 (PPET-1) and prepro-VIC (PPVIC) genes, base...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00342-4

    authors： Uchide T,Adur J,Saida K

    更新日期：2001-11-30 00:00:00

  • Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA.

    abstract：:Aptamers are single-stranded DNA or RNA oligonucleotides, which have been emerging as recognition elements in disease diagnostics and food control, including the detection of bacterial toxins. In this study, we employed the semi-automated just in time-selection to identify aptamers that bind to cholera toxin (CT) with...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2018.01.012

    authors： Frohnmeyer E,Frisch F,Falke S,Betzel C,Fischer M

    更新日期：2018-03-10 00:00:00

  • Selective enhancement of autotrophic acetate production with genetically modified Acetobacterium woodii.

    abstract：:Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the u...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.03.005

    authors： Straub M,Demler M,Weuster-Botz D,Dürre P

    更新日期：2014-05-20 00:00:00

  • Amperometric immunosensor based on toluidine blue/nano-Au through electrostatic interaction for determination of carcinoembryonic antigen.

    abstract：:A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.11.023

    authors： Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Y

    更新日期：2006-05-29 00:00:00

  • Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by a coculture of Pseudomonas putida and Pseudomonas fluorescens immobilized in a fibrous-bed bioreactor.

    abstract：:A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-be...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(98)00166-7

    authors： Shim H,Yang ST

    更新日期：1999-01-22 00:00:00

  • Genome-wide analysis of the L-methionine biosynthetic pathway in Corynebacterium glutamicum by targeted gene deletion and homologous complementation.

    abstract：:The genome sequence of Corynebacterium glutamicum, a gram-positive soil bacterium widely used as an amino acid producer, was analyzed by a similarity-based approach to elucidate the pathway for the biosynthesis of L-methionine. The functions of candidate ORFs were derived by gene deletion and, if necessary, by homolog...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(03)00158-5

    authors： Rückert C,Pühler A,Kalinowski J

    更新日期：2003-09-04 00:00:00