Abstract:
:ACC deaminase producing Plant growth promoting rhizobacteria (PGPR) offers a great promise for ameliorating the negative impacts of salinity stress manifested on plants. In this context, 28 rhizospheric bacteria associated with ACC deaminase potential (198-1069 nmol α-ketobutyrate mg protein-1 h-1) were isolated from 5 different islands of Lakshadweep, union territory, India- Agatti, Kavaratti, Bangaram, Kadmat, and Thinnakara islands using DF-minimal medium. The diversity of cultivable ACC deaminase producing bacteria was analysed by PCR-RFLP (Restriction Fragment Length Polymorphism) method using three endonucleases AluI, MspI and HaeIII which led to the grouping of these isolates into six clusters at 80 % similarity index. Subsequently, isolates were functionally characterized for various PGP traits such that indole-3-acetic acid (IAA) production (∼10-80 μg mL-1); 16 isolates had phosphate solubilizing potential ranging from ∼19 to 88 P mg L-1 ; siderophore and ammonia production abilities were observed in 5 and 24 isolates, respectively while two strains tolerated up to 8% NaCl. Phylogenetic analysis of 16S rRNA gene sequences of representative strain from each cluster revealed that twenty-eight ACC deaminase producing PGPR belong to eight distinct genera: Pseudomonas, Bacillus, Azospirillum, Azotobacter, Escherichia, Paenibacillus, Burkholderia, and Klebsiella. Two isolates, CO1 (Pseudomonas putida) and CO8 (Bacillus paramycoides) were evaluated for plant growth promoting effects on French bean (Phaseolus vulgaris) under salinity (100 mM NaCl) stress. Both the selected isolates in consortium form significantly increased the root length, shoot length, root fresh and dry weight, shoot fresh and dry weight of French bean seedlings exposed to salinity stress, compared to non-inoculated control plants. The co-inoculation with selected strains CO1 and CO8 has significantly improved chlorophyll concentration, relative water content, membrane stability index, gas exchange parameters including net photosynthesis rate (PN), stomatal conductance (gs), transpiration rate (E) and water use efficiency of French bean plants by ∼100 %, ∼85 %, ∼40 %, ∼198 %, ∼80 %, ∼70 % and ∼75 %, respectively under saline conditions in comparison with non-inoculated plants. Moreover, the consortium treated French bean plants showed lower levels of stress-induced ethylene by 38 %, electrolyte leakage and Malondialdehyde (MDA) content by ∼15 % under salt stress compared to non-inoculated ones. This study unveiled the potential of halotolerant strains, Pseudomonas putida and Bacillus paramycoides as French bean biofertilizers in mitigating the adverse effects of salinity in plant growth in sustainable agriculture.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Pandey S,Gupta Sdoi
10.1016/j.jbiotec.2020.10.024subject
Has Abstractpub_date
2020-12-20 00:00:00pages
183-197eissn
0168-1656issn
1873-4863pii
S0168-1656(20)30294-7journal_volume
324pub_type
杂志文章abstract::Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. The wild-type strain NCPPB382 carries two plasmids, pCM1 and pCM2. The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no dise...
journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.jbiotec.2003.07.011
更新日期:2003-12-19 00:00:00
abstract::While phase I and phase II drug metabolites are important for drug development and toxicity studies, e.g. in the context of metabolites in safety testing (MIST), they are often not commercially available and their classical chemical synthesis can be cumbersome. Therefore, a biotechnological production of drug metaboli...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2010.08.004
更新日期:2010-10-01 00:00:00
abstract::A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes. They work with one or more cis-acting sites termed centromere-like sequences to ensure better than random predivisional partitioning of the DNA molecule that encodes them. The centromere-like sequences nucl...
journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0168-1656(01)00293-0
更新日期:2001-09-13 00:00:00
abstract::In this work, the ethanolysis of triglycerides catalyzed by immobilized lipase was studied, focusing on the secondary reaction of acyl migration. The catalytic tests were performed in a solvent-free reaction medium using Novozym 435 as biocatalyst. The selected experimental variables were biocatalyst loading (5-20mg),...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.01.001
更新日期:2016-02-20 00:00:00
abstract::A new and simple method was devised to obtain immunoglobulin VH genes directly from primary cultured B cells specific for a short peptide. Peptide-specific B cells were separated from splenocytes of peptide-immunized BALB/c mice with antigen-coated magnetic beads, and were cloned by a limitedly diluted culture in the ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(96)01506-4
更新日期:1996-08-20 00:00:00
abstract::Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and streptavidin modification of proteins or small molecules is widely used for biotechnology tool. Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We focused on sortase A, a transpeptidase from ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2011.01.008
更新日期:2011-03-10 00:00:00
abstract::Often, degradability under anaerobic conditions is desirable for plastics claimed to be biodegradable, e.g. in anaerobic biowaste treatment plants, landfills and in natural anaerobic sediments. The biodegradation of the natural polyesters poly(beta-hydroxybutyrate) (PHB), poly(beta-hydroxybutyrate-co-11.6%-beta-hydrox...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(00)00406-5
更新日期:2001-03-30 00:00:00
abstract::(+)-Nootkatone is a natural ingredient that occurs in grapefruit and certain other plants and is responsible for the characteristic smell of grapefruit. Due to its versatile applications in the flavor and fragrance industry as well as its application in some medical uses it recruits the interests of academic research ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2019.05.017
更新日期:2019-08-10 00:00:00
abstract::One important question in microbiome analysis is how to assess the homogeneity of the microbial composition in a given environment, with respect to a given analysis method. Do different microbial samples taken from the same environment follow the same taxonomic distribution of organisms, or the same distribution of fu...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.10.020
更新日期:2017-05-20 00:00:00
abstract::A sensitive and highly selective method for the simultaneous determination of purine bases and their nucleosides is proposed. An amperometric flow-injection system with the two immobilized enzyme reactors (guanase immobilized reactor and purine nucleoside phosphorylase/xanthine oxidase co-immobilized reactor) is used ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(90)90021-3
更新日期:1990-04-01 00:00:00
abstract::Dual nutrient limited growth, the control of the cell growth rate (kinetic aspect) or the restriction of the amount of biomass (stoichiometric aspect) by two nutrients at the same time, is a relatively unknown ability of the microorganisms and consequently, still not mentioned in textbooks to date. Nevertheless, multi...
journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.jbiotec.2004.03.030
更新日期:2004-09-30 00:00:00
abstract::Biodegradable scaffolds are of great value in tissue engineering. We have developed a method for fabricating patient-specific vascular scaffolds from a biocompatible and biodegradable polymer, poly(L-lactide-co-epsilon-caprolactone). This method's usefulness is due to flexibility in the choice of materials and vascula...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.08.017
更新日期:2008-01-20 00:00:00
abstract::To study the metaproteome of a biogas-producing microbial community, fermentation samples were taken from an agricultural biogas plant for microbial cell and protein extraction and corresponding metagenome analyses. Based on metagenome sequence data, taxonomic community profiling was performed to elucidate the composi...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.06.014
更新日期:2016-08-10 00:00:00
abstract::The aim of this study is to evaluate the utility of using aerobic acetate-fed microbial granules as a starting seed to rapidly develop stable aerobic phenol-degrading granules. Aerobic granules were first cultivated in four sequencing batch reactors with acetate as sole carbon source at a loading rate of 3.8 kg m(-3) ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2004.09.008
更新日期:2005-02-23 00:00:00
abstract::4-vinylguaiacol, a kind of volatile phenolic compound with tobacco flavor, is widely used as a component of edible flavor and intermediate of medicine. Ferulic acid is usually used as substrate for the biosynthesis of 4-vinylguaiacol. However, the price of ferulic acid is high, leading to high production cost. In this...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2018.07.021
更新日期:2018-09-10 00:00:00
abstract::One of the main limitations in bacterial polyhydroxyalkanoate (PHA) production with mixed cultures is the fact that primarily polyhydroxybutyrate (PHB) homopolymers are generated from acetate as the main carbon source, which is brittle and quite fragile. The incorporation of different 3-hydroxyalkanoate (HA) component...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.01.036
更新日期:2007-05-01 00:00:00
abstract::Efficient dynamic interactions among cofactor, enzymes and substrate molecules are of primary importance for multi-step enzymatic reactions with in situ cofactor regeneration. Here we showed for the first time that the above dynamic interactions could be significantly intensified by exerting an external alternating ma...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2013.05.016
更新日期:2013-10-20 00:00:00
abstract::The use of enzymes in organic solvents offers a great opportunity for the highly selective synthesis of complex organic compounds. In this study we investigate the POXylation of several enzymes with different polyoxazolines ranging from the hydrophilic poly(2-methyl-oxazoline) (PMOx) to the hydrophobic poly(2-heptyl-o...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2014.03.035
更新日期:2014-07-10 00:00:00
abstract::A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) is a novel family of extracellular proteases supposedly involved in inflammation, angiogenesis, development and coagulation. To overexpress the active ADAMTS proteins, we designed a chimeric molecule composed of a catalytic domain of ADAMTS-1...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(02)00243-2
更新日期:2003-02-13 00:00:00
abstract::New antifungal compounds that circumvent the resistance of the pathogen by directly damaging yeast cell surface structures are promising agents for the treatment of fungal infections, due to their different mechanism of action from current clinically used antifungal drugs. We present here a rapid and cost-effective fl...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.06.023
更新日期:2016-09-10 00:00:00
abstract::Procedures were developed for 'self-immobilisation' of Solanum aviculare cells to eliminate the need for artificial immobilisation supports. Depending on the cytokinin used in liquid medium, compact aggregates 0.4-2.0 cm in diameter were formed without dispersed cells also being present. Histochemical analysis showed ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(91)90077-9
更新日期:1991-06-01 00:00:00
abstract::The efficiency of protein synthesis for glyceraldehyde-3-phosphate dehydrogenase (G3PDH) was examined with several in vitro coupled transcription/translation protein synthesis systems based on Escherichia coli lysate, wheat germ, or reticulocyte lysate, and an in vitro translation system based on wheat germ extract. A...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.08.008
更新日期:2008-01-20 00:00:00
abstract::We report on the isolation and biochemical characterization of a novel, cold-active and metagenome-derived lipase with a high stereo-selectivity for pharmaceutically important substrates. The respective gene was isolated from a cosmid library derived from oil contaminated soil and designated lipCE. The deduced aa sequ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.05.015
更新日期:2007-07-15 00:00:00
abstract::The poly(beta-hydroxybutyrate) (PHB) biosynthetic genes of Ralstonia eutropha that are organized in a single operon (phaCAB) have been cloned in Escherichia coli, where the expression of the genes in the wild-type pha operon from plasmid pTZ18U-PHB leads to the formation of 50-80% PHB/celldry mass when the cells are g...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(98)00127-8
更新日期:1998-10-27 00:00:00
abstract::In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2012.12.002
更新日期:2013-02-20 00:00:00
abstract::Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2008.09.014
更新日期:2009-01-15 00:00:00
abstract::The polysaccharide xanthan which is produced by the γ-proteobacterium Xanthomonas campestris is used as a food thickening agent and rheologic modifier in numerous food, cosmetics and technical applications. Its great commercial importance stimulated biotechnological approaches to optimize the xanthan production. By ta...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.11.032
更新日期:2017-09-10 00:00:00
abstract::This study aimed to produce a cellulase blend and to evaluate its application in a simultaneous saccharification and fermentation (SSF) process for second generation ethanol production from sugar cane bagasse. The sugar cane bagasse was subjected to pretreatments (diluted acid and alkaline), as for disorganizing the l...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2012.10.014
更新日期:2013-01-10 00:00:00
abstract::beta-galactosidase reporter plasmids containing different viral or minimal promoters are commonly used to correct variable transfection efficiencies in transient transfection experiments. The transcriptional activity of these promoters is thought to be stable under most circumstances. To determine if expression of bet...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(01)00277-2
更新日期:2001-07-12 00:00:00
abstract::New methods for an extended physiological characterization of yeast at a microtiter plate scale were applied to 27 deletion mutants of Saccharomyces cerevisiae cultivated on glucose and galactose as sole carbon sources. In this way, specific growth rates, specific rates of glucose consumption and ethanol production we...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.08.043
更新日期:2007-12-01 00:00:00