Effects of oil substrate supplementation on production of prodigiosin by Serratia nematodiphila for dye-sensitized solar cell.

Abstract:

:Bacterial pigments are potential substitute of chemical photosensitizer for dye-sensitized solar cell (DSSC) due to its non-toxic property and cost-effective production from microbial fermentation. Serratia nematodiphila YO1 was isolated from waterfall in Malaysia and identified using 16S ribosomal RNA. Characterization of the red pigment produced by the bacteria has confirmed the pigment as prodigiosin. Prodigiosin was produced from the fermentation of the bacteria in the presence of different oil substrates. Palm oil exhibited the best performance of cell growth and equivalent prodigiosin yield compared to olive oil and peanut oil. Prodigiosin produced with palm oil supplementation was 93 mg/l compared to 7.8 mg/l produced without supplementation, which recorded 11.9 times improvement. Specific growth rate of the cells improved 1.4 times when palm oil was supplemented in the medium. The prodigiosin pigment produced showed comparable performance as a DSSC sensitizer by displaying an open circuit voltage of 336.1 mV and a maximum short circuit current of 0.098 mV/cm2. This study stands a novelty in proving that the production of prodigiosin is favorable in the presence of palm oil substrate with high saturated fat content, which has not been studied before. This is also among the first bacterial prodigiosin tested as photosensitizer for DSSC application.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Abdul Manas NH,Chong LY,Tesfamariam YM,Zulkharnain A,Mahmud H,Abang Mahmod DS,Mohamad Fuzi SFZ,Wan Azelee NI

doi

10.1016/j.jbiotec.2020.04.011

subject

Has Abstract

pub_date

2020-06-20 00:00:00

pages

16-26

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(20)30099-7

journal_volume

317

pub_type

杂志文章
  • Genome improvement of the acarbose producer Actinoplanes sp. SE50/110 and annotation refinement based on RNA-seq analysis.

    abstract::Actinoplanes sp. SE50/110 is the natural producer of acarbose, which is used in the treatment of diabetes mellitus type II. However, until now the transcriptional organization and regulation of the acarbose biosynthesis are only understood rudimentarily. The genome sequence of Actinoplanes sp. SE50/110 was known befor...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.04.013

    authors: Wolf T,Schneiker-Bekel S,Neshat A,Ortseifen V,Wibberg D,Zemke T,Pühler A,Kalinowski J

    更新日期:2017-06-10 00:00:00

  • Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes.

    abstract::The expression of heterologous proteins in microorganisms using genetic recombination is still the high point in the development and exploitation of modern biotechnology. People can produce bioactive proteins from relatively cheap culture medium instead of expensive extraction. Host cell systems for the expression of ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jbiotec.2006.07.012

    authors: Yin J,Li G,Ren X,Herrler G

    更新日期:2007-01-10 00:00:00

  • Monitoring Chinese hamster ovary cell culture by the analysis of glucose and lactate metabolism.

    abstract::Monitoring cell growth is crucial to the success of an animal cell culture process that can be accomplished by a variety of direct or indirect methodologies. Glucose is a major carbon and energy source for cultured mammalian cells in most cases, but glycolytic metabolism often results in the accumulation of lactate. G...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.05.016

    authors: Tsao YS,Cardoso AG,Condon RG,Voloch M,Lio P,Lagos JC,Kearns BG,Liu Z

    更新日期:2005-08-22 00:00:00

  • Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by a coculture of Pseudomonas putida and Pseudomonas fluorescens immobilized in a fibrous-bed bioreactor.

    abstract::A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-be...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(98)00166-7

    authors: Shim H,Yang ST

    更新日期:1999-01-22 00:00:00

  • Solid-state fermentative production of aroma esters by Myroides sp. ZB35 and its complete genome sequence.

    abstract::Consumers prefer biotechnological food products with high nutritional values and good flavors. Solid-state fermentation is a commonly used technique with a long history. In the present study, Myroides sp. ZB35 was used in solid-state fermentative production of aroma volatiles on a rice medium. Using the headspace soli...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.02.003

    authors: Xiao Z,Liang W,Zhu X,Zhao JY

    更新日期:2017-03-10 00:00:00

  • Heterologous production of different styrene oxide isomerases for the highly efficient synthesis of phenylacetaldehyde.

    abstract::The styrene oxide isomerase (SOI, StyC) represents a key enzyme of the styrene-degrading pathway and has been discussed as promising biocatalyst during recent studies. The enzyme enables the synthesis of pure phenylacetaldehyde from styrene oxide. In this study the native as well as the corresponding codon-optimized g...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.04.038

    authors: Oelschlägel M,Richter L,Stuhr A,Hofmann S,Schlömann M

    更新日期:2017-06-20 00:00:00

  • DNA vaccination of mice with a plasmid encoding Puumala hantavirus nucleocapsid protein mimics the B-cell response induced by virus infection.

    abstract::Inoculation of naked DNA has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. To study the humoral immune response induced by DNA vaccination we cloned the entire nucleocapsid protein-encoding sequence of the Puumala hantavirus strain Vranica/Hällnäs into th...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(00)00329-1

    authors: Koletzki D,Schirmbeck R,Lundkvist A,Meisel H,Krüger DH,Ulrich R

    更新日期:2001-11-17 00:00:00

  • Gene prediction and gene classes in Arabidopsis thaliana.

    abstract::Gene prediction methods for eukaryotic genomes still are not fully satisfying. One way to improve gene prediction accuracy, proven to be relevant for prokaryotes, is to consider more than one model of genes. Thus, we used our classification of Arabidopsis thaliana genes in two classes (CU(1) and CU(2)), previously del...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(00)00196-6

    authors: Mathé C,Déhais P,Pavy N,Rombauts S,Van Montagu M,Rouzé P

    更新日期:2000-03-31 00:00:00

  • Marine actinomycetes as an emerging resource for the drug development pipelines.

    abstract::Many representatives of the order Actinomycetales are prolific producers of thousands of biologically active secondary metabolites. Actinomycetes from terrestrial sources have been studied and screened since the 1950s, yielding many important anti-infective and anti-cancer drugs. However, frequent re-discovery of the ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jbiotec.2011.06.002

    authors: Zotchev SB

    更新日期:2012-04-30 00:00:00

  • ARDesigner: a web-based system for allosteric RNA design.

    abstract::RNA molecules play vital informational, structural, and functional roles in molecular biology, making them ideal targets for synthetic biology. However, several challenges remain for engineering novel allosteric RNA molecules, and the development of efficient computational design techniques is vitally needed. Here we ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2010.10.067

    authors: Shu W,Liu M,Chen H,Bo X,Wang S

    更新日期:2010-12-01 00:00:00

  • An efficient gene transfer system for hematopoietic cell line using transient and stable vectors.

    abstract::The hematopoietic system represents an interesting model for gene transfer protocols. Here, we have evaluated the efficiency of a gene transfer system using the polycationic compound SuperFect (Qiagen) and the K562 hematopoietic cell line. Transient and stable vectors carrying the enhanced green fluorescent protein (E...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(01)00276-0

    authors: Teixeira LA,Fricke CH,Bonorino CB,Bogo MR,Nardi NB

    更新日期:2001-06-15 00:00:00

  • Transcriptomic changes during tuber dormancy release process revealed by RNA sequencing in potato.

    abstract::Potato tuber dormancy release is a critical development process that allows potato to produce new plant. The first Illumina RNA sequencing to generate the expressed mRNAs at dormancy tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) was performed. We identified 26,639 genes including 5,912 (3,450 up-re...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.01.019

    authors: Liu B,Zhang N,Wen Y,Jin X,Yang J,Si H,Wang D

    更新日期:2015-03-20 00:00:00

  • Complete genome sequence of Rufibacter tibetensis strain 1351, a radiation-resistant bacterium from Tibet plateau.

    abstract::Rufibacter tibetensis strain 1351, isolated from the soil of the Tibet plateau of China, belongs to the family of Cytophagaceae. It is a red-pigmented, gram-negative, strictly aerobic and rod-shaped bacterium and shows resistance to UV radiation. Here, we report its complete genome sequence, which can help us find the...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.10.015

    authors: Zhang Y,Yu C,Zhou M,Tang J,Li X,Wang Z,Li Z,Yao J,Li P,Zheng G,Chen X,Dai J

    更新日期:2015-12-20 00:00:00

  • Effect of introducing a disulphide bond between the A and C domains on the activity and stability of Saccharomycopsis fibuligera R64 α-amylase.

    abstract::Native enzyme and a mutant containing an extra disulphide bridge of recombinant Saccharomycopsis fibuligera R64 α-amylase, designated as Sfamy01 and Sfamy02, respectively, have successfully been overexpressed in the yeast Pichia pastoris KM71H. The purified α-amylase variants demonstrated starch hydrolysis resulting i...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.12.002

    authors: Natalia D,Vidilaseris K,Ismaya WT,Puspasari F,Prawira I,Hasan K,Fibriansah G,Permentier HP,Nurachman Z,Subroto T,Dijkstra BW,Soemitro S

    更新日期:2015-02-10 00:00:00

  • Purification of biomevalonate from fermentation broth and conversion of biomevalonate into biomevalonolactone.

    abstract::Mevalonate (MVA) is a key compound of living organisms including bacteria, plants, and humans. MVA and mevalonolactone (MVL), a lactonized form of MVA, are important for pharmaceutical, cosmeceutical, and biotechnological applications. Although (R, S)-MVA with 50% enantiomeric purity is mainly produced by chemical syn...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.08.017

    authors: Kim JW,Yoon HC,Kwon SJ,Lee BY,Lee PC

    更新日期:2017-10-10 00:00:00

  • Towards the in vivo production of tocotrienol compounds: engineering of a plasmid-free Escherichia coli strain for the heterologous synthesis of 2-methyl-6-geranylgeranyl-benzoquinol.

    abstract::The vitamin E family consists of 4 tocopherol and 4 tocotrienol compounds. During recent years, tocotrienols have gained increased interest due to their biological activities that are beyond the vitamin E activity. Here we report the engineering of plasmid-free Escherichia coli strains for an efficient synthesis of 2-...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2012.08.010

    authors: Ghanegaonkar S,Conrad J,Beifuss U,Sprenger GA,Albermann C

    更新日期:2012-12-15 00:00:00

  • Circadian rhythms in the cell cycle and biomass composition of Neochloris oleoabundans under nitrogen limitation.

    abstract::The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.07.016

    authors: de Winter L,Schepers LW,Cuaresma M,Barbosa MJ,Martens DE,Wijffels RH

    更新日期:2014-10-10 00:00:00

  • Expression and immunogenicity assessment of a plant-made immunogen targeting the cytotoxic T-lymphocyte associated antigen-4: a possible approach for cancer immunotherapy.

    abstract::Cancer immunotherapy is a promising intervention to fight against this global health problem. In particular targeting immune checkpoints, such as cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and programmed-death protein 1 (PD-1), by specific monoclonal antibodies is a current treatment for many malignances. A ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2021.01.016

    authors: Yiemchavee S,Wong-Arce A,Romero-Maldonado A,Shanmugaraj B,Monsivais-Urenda A,Phoolcharoen W,Rosales-Mendoza S

    更新日期:2021-01-21 00:00:00

  • Albumin-binding domain from Streptococcus zooepidemicus protein Zag as a novel strategy to improve the half-life of therapeutic proteins.

    abstract::Recombinant antibody fragments belong to the promising class of biopharmaceuticals with high potential for future therapeutic applications. However, due to their small size they are rapidly cleared from circulation. Binding to serum proteins can be an effective approach to improve pharmacokinetic properties of short h...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.05.017

    authors: Cantante C,Lourenço S,Morais M,Leandro J,Gano L,Silva N,Leandro P,Serrano M,Henriques AO,Andre A,Cunha-Santos C,Fontes C,Correia JDG,Aires-da-Silva F,Goncalves J

    更新日期:2017-07-10 00:00:00

  • Improved properties of micronized genetically modified flax fibers.

    abstract::The aim of this study was to investigate the effect of micronization on the compound content, crystalline structure and physicochemical properties of fiber from genetically modified (GM) flax. The GM flax was transformed with three bacterial (Ralstonia eutropha) genes coding for enzymes of polyhydroxybutyrate (PHB) sy...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.01.002

    authors: Dymińska L,Szatkowski M,Wróbel-Kwiatkowska M,Zuk M,Kurzawa A,Syska W,Gągor A,Zawadzki M,Ptak M,Mączka M,Hanuza J,Szopa J

    更新日期:2012-12-15 00:00:00

  • Immobilization of Aspergillus terreus lipase in self-assembled hollow nanospheres for enantioselective hydrolysis of ketoprofen vinyl ester.

    abstract::The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate-graft-poly(ethylene glycol)/α-cyclodextrins (Alg-g-PEG/α-CD) spheres as enzyme immobilization carriers. The morphology and size o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.11.032

    authors: Hu C,Wang N,Zhang W,Zhang S,Meng Y,Yu X

    更新日期:2015-01-20 00:00:00

  • Design and prototyping of a chip-based multi-micro-organoid culture system for substance testing, predictive to human (substance) exposure.

    abstract::Dynamic miniaturized human multi-micro-organ bioreactor systems are envisaged as a possible solution for the embarrassing gap of predictive substance testing prior to human exposure. A rational approach was applied to simulate and design dynamic long-term cultures of the smallest possible functional human organ units,...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2010.02.001

    authors: Sonntag F,Schilling N,Mader K,Gruchow M,Klotzbach U,Lindner G,Horland R,Wagner I,Lauster R,Howitz S,Hoffmann S,Marx U

    更新日期:2010-07-01 00:00:00

  • Regulation of the pigment optical density of an algal cell: filling the gap between photosynthetic productivity in the laboratory and in mass culture.

    abstract::An increasing number of investors is looking at algae as a viable source of biofuels, beside cultivation for human/animal feeding or to extract high-value chemicals and pharmaceuticals. However, present biomass productivities are far below theoretical estimations implying that a large part of the available photosynthe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2012.02.021

    authors: Formighieri C,Franck F,Bassi R

    更新日期:2012-11-30 00:00:00

  • Enhanced expression of human prostaglandin H synthase-2 in the yeast Pichia pastoris and removal of the C-terminal tag with bovine carboxypeptidase A.

    abstract::Vertebrate prostaglandin H synthases (PGHSs) are membrane-bound disulphide-containing hemoglycoproteins. Therefore, eukaryotic expression systems are required for the production of recombinant PGHSs. Recently we announced the expression of human PGHS-2 (hPGHS-2) in the yeast Pichia pastoris. Here we report improved pr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.06.015

    authors: Kukk K,Samel N

    更新日期:2016-08-10 00:00:00

  • Hydrophobicity engineering to facilitate surface display of heterologous gene products on Staphylococcus xylosus.

    abstract::Protein engineering has been employed to investigate the effect of specific amino acid changes on the targeting of heterologous proteins to the outer cell surface of the Gram-positive bacterium Staphylococcus xylosus. Three different variants, corresponding to a 101 amino acid region of the major glycoprotein (G prote...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(95)00081-z

    authors: Nguyen TN,Gourdon MH,Hansson M,Robert A,Samuelson P,Libon C,Andréoni C,Nygren PA,Binz H,Uhlén M

    更新日期:1995-10-16 00:00:00

  • L-Lysine biosynthetic pathway of Methylophilus methylotrophus and construction of an L-lysine producer.

    abstract::Previously, we showed that the enzymes aspartokinase (AK) and dihydrodipicolinate synthase (DDPS), which are involved in L-lysine biosynthesis in the Gram-negative obligate methylotroph Methylophilus methylotrophus AS1, were inhibited by allosteric effectors, including L-lysine. To elucidate further the regulation of ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.12.026

    authors: Tsujimoto N,Gunji Y,Ogawa-Miyata Y,Shimaoka M,Yasueda H

    更新日期:2006-07-13 00:00:00

  • Characterization of stress and protein turnover from protein overexpression in fed-batch E. coli cultures.

    abstract::A structured kinetic model that accounts for proteolytic degradation due to recombinant protein overexpression is introduced and its performance evaluated by comparison with previously reported fed-batch experimental data. This mathematical model contains an additional pool for a generic key precursor (in our case phe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(99)00014-0

    authors: Ramírez DM,Bentley WE

    更新日期:1999-05-28 00:00:00

  • On-line estimation of biomass, glucose and ethanol in Saccharomyces cerevisiae cultivations using in-situ multi-wavelength fluorescence and software sensors.

    abstract::Batch bioreactor cultivations using Saccharomyces cerevisiae at high (190-305 gl(-1) glucose) or low (21-25 gl(-1) glucose) gravity conditions were monitored on-line using multi-wavelength fluorescence (MWF) and standard monitoring sensors. Partial least squares models were calibrated for the prediction of cell dry we...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2009.08.018

    authors: Odman P,Johansen CL,Olsson L,Gernaey KV,Lantz AE

    更新日期:2009-10-26 00:00:00

  • The complete genome sequence of a lactic acid bacterium Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T).

    abstract::Leuconostoc species are widespread in the natural environment and play an important role in several types of industrial and food fermentation processes. Here, we report the 1,854,727-bp complete genome sequence of the Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T). ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.12.009

    authors: Park GS,Hong SJ,Jung BK,Lee C,Park CK,Shin JH

    更新日期:2016-02-10 00:00:00

  • Improvement of a d-biotin-hyperproducing recombinant strain of Serratia marcescens.

    abstract::We previously reported that a recombinant strain, SB412(pLGM304), was constructed from acidomycin-resistant mutants of Serratia marcescens and produced 200 mg of d-biotin per liter of a medium containing sucrose and urea (Sakurai et al., 1993a, b). In the present work, we intended to improve the d-biotin production. B...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(94)90024-8

    authors: Sakurai N,Imai Y,Masuda M,Komatsubara S,Tosa T

    更新日期:1994-07-29 00:00:00