Abstract:
:A structured kinetic model that accounts for proteolytic degradation due to recombinant protein overexpression is introduced and its performance evaluated by comparison with previously reported fed-batch experimental data. This mathematical model contains an additional pool for a generic key precursor (in our case phenylalanine), an improved IPTG transport term, a phenylalanine transport term, and a variable protein turnover expression that accounts for proteolytic activity. The model predictions concerning proteolytic activity, glucose level, and cell growth are in very good agreement with an amino acid depletion hypothesis. Cultures exposed to greater stress showed higher and/or longer proteolysis, whereas less overall proteolytic activity was observed when the effect of induction was somewhat ameliorated.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Ramírez DM,Bentley WEdoi
10.1016/s0168-1656(99)00014-0subject
Has Abstractpub_date
1999-05-28 00:00:00pages
39-58issue
1-3eissn
0168-1656issn
1873-4863pii
S0168-1656(99)00014-0journal_volume
71pub_type
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