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Gene prediction and gene classes in Arabidopsis thaliana.

Abstract：

:Gene prediction methods for eukaryotic genomes still are not fully satisfying. One way to improve gene prediction accuracy, proven to be relevant for prokaryotes, is to consider more than one model of genes. Thus, we used our classification of Arabidopsis thaliana genes in two classes (CU(1) and CU(2)), previously delineated according to statistical features, in the GeneMark gene identification program. For each gene class, as well as for the two classes combined, a Markov model was developed (respectively, GM-CU(1), GM-CU(2) and GM-all) and then used on a test set of 168 genes to compare their respective efficiency. We concluded from this analysis that GM-CU(1) is more sensitive than GM-CU(2) which seems to be more specific to a gene type. Besides, GM-all does not give better results than GM-CU(1) and combining results from GM-CU(1) and GM-CU(2) greatly improve prediction efficiency in comparison with predictions made with GM-all only. Thus, this work confirms the necessity to consider more than one gene model for gene prediction in eukaryotic genomes, and to look for gene classes in order to build these models.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Mathé C,Déhais P,Pavy N,Rombauts S,Van Montagu M,Rouzé P

doi

10.1016/s0168-1656(00)00196-6

subject

Has Abstract

pub_date

2000-03-31 00:00:00

pages

293-9

issue

3

eissn

0168-1656

issn

1873-4863

pii

S0168165600001966

journal_volume

78

pub_type

杂志文章

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