Abstract：
:The hematopoietic system represents an interesting model for gene transfer protocols. Here, we have evaluated the efficiency of a gene transfer system using the polycationic compound SuperFect (Qiagen) and the K562 hematopoietic cell line. Transient and stable vectors carrying the enhanced green fluorescent protein (EGFP) reporter gene were employed. The stable vector was constructed based on Epstein-Barr virus sequences such as EBV oriP (origin of replication) and EBNA (EBV nuclear antigen)-1, both for DNA replication. The transfection efficiency of the viable cells was estimated by flow cytometry at approximately 98% for transient and stable vectors. Transiently transfected cells presented optimal EGFP expression until day 2 when fluorescence started to decrease. In contrast, stable transfectants continuously expressed the marker gene product for 10 weeks in the presence of G418. Our results represent an efficient gene transfer method for K562 hematopoietic cells and may be used as an alternative approach for further gene transfer studies involving hematopoietic cells.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Teixeira LA,Fricke CH,Bonorino CB,Bogo MR,Nardi NBdoi
10.1016/s0168-1656(01)00276-0subject
Has Abstractpub_date
2001-06-15 00:00:00pages
159-65issue
2eissn
0168-1656issn
1873-4863pii
S0168165601002760journal_volume
88pub_type
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(93)90148-g
更新日期：1993-09-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：2000-09-29 00:00:00
abstract：:Peptide synthesis was carried out in a variety of organic solvents with low contents of water. The enzyme was deposited on the support material, celite, from an aqueous buffer solution. After evaporation of the water the biocatalyst was suspended in the reaction mixtures. The chymotrypsin-catalyzed reaction between Z-...
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pub_type： 杂志文章
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更新日期：1999-03-26 00:00:00
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journal_title：Journal of biotechnology
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abstract：:Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2...
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abstract：:Human interleukin (IL)-29 is the latest member of the class II cytokine family. However, as a result of lacking efficient method to generate relatively large quantity of IL-29, little is known of its functions in man. In the present study, an Escherichia coli expression system for the rapid expression of the human IL-...
journal_title：Journal of biotechnology
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更新日期：2006-04-10 00:00:00
