Abstract：
:Human interleukin (IL)-29 is the latest member of the class II cytokine family. However, as a result of lacking efficient method to generate relatively large quantity of IL-29, little is known of its functions in man. In the present study, an Escherichia coli expression system for the rapid expression of the human IL-29 gene was developed. It involved of cloning IL-29 gene into the pET-44 Ek/LIC vector, which allowed expression of IL-29 with a fusion tag consisting of the NusA protein, polyhistidine and S peptide (Nus-His-S-tag), and introducing a thrombin recognition site between the fusion tag and IL-29. The expressed fusion protein was purified by S-protein agarose affinity chromatography, and the fusion tag was removed from recombinant IL-29 by cleavage with thrombin. The purified IL-29 appeared a single band on SDS-PAGE, and the yield of IL-29 was 60 mg from 1 l of bacterial culture. N-terminal sequencing confirmed the identity of the purified protein. The recombinant IL-29 showed specific antiviral activity that was comparable to the commercially available IFN alfa-2b preparation.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Li M,He Sdoi
10.1016/j.jbiotec.2005.11.019subject
Has Abstractpub_date
2006-04-10 00:00:00pages
334-40issue
3eissn
0168-1656issn
1873-4863pii
S0168-1656(05)00765-0journal_volume
122pub_type
杂志文章abstract：:Combining the isochorismate synthase EntC and the chorismatase FkbO in a sequential enzyme cascade provides a useful system for the biocatalytic production and subsequent purification of isochorismate from an isochorismate/chorismate mixture. FkbO has a strict preference for chorismate - isochorismate is not accepted ...
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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更新日期：2015-03-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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doi：10.1016/j.jbiotec.2008.06.002
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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更新日期：2002-07-03 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：2014-12-20 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：2007-04-30 00:00:00
abstract：:Conventional approaches to differential gene expression comparisons assume equal cellular RNA content among experimental conditions. We demonstrate that this assumption should not be universally applied because total RNA yield from a set number of cells varies among experimental treatments of the same Chinese Hamster ...
journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2014-11-10 00:00:00
