Differential induction of autophagy in caspase-3/7 down-regulating and Bcl-2 overexpressing recombinant CHO cells subjected to sodium butyrate treatment.

abstract：:In this report, we describe the results of a rat pituitary cell culture experiment done on STS-65 in which the effect of cell feeding on the release of the six anterior pituitary hormones was studied. We found complex microgravity-related interactions between the frequency of cell feeding and the quantity and quality ...

journal_title：Journal of biotechnology

authors： Hymer WC,Grindeland RE,Salada T,Cenci R,Krishnan K,Mukai C,Nagaoka S

更新日期：1996-06-27 00:00:00

abstract：:Nanobiotechnological improvements defined on the utilization of biological materials and principles have enormously partaken to revolutionize physical, chemical, and biological sciences. However, the exploration of plant nanobiotechnology is still in its outset. The search for novel tools to monitor plant biomolecules...

journal_title：Journal of biotechnology

authors： Ambrin G,Kausar H,Ahmad A

更新日期：2020-11-10 00:00:00

abstract：:Our unpublished experimental results of fractional factorial experiments showed that the significant external factors affecting high pressure processing (HPP) inactivation were pressure, temperature and pressure holding time. Based on these results, response surface methodology (RSM) was employed in the present work a...

journal_title：Journal of biotechnology

authors： Gao YL,Ju XR,Jiang HH

更新日期：2006-09-18 00:00:00

abstract：:In Salvia miltiorrhiza hairy root cultures, the desired secondary metabolites diterpenoid tanshinones are normally produced at low yields and stored within the roots. To enhance tanshinone production and the secondary product recovery, we employed three means, elicitation with a yeast elicitor (YE), in situ adsorption...

journal_title：Journal of biotechnology

authors： Yan Q,Hu Z,Tan RX,Wu J

更新日期：2005-10-10 00:00:00

abstract：:Tissue engineering involves ex vivo seeding of anchorage-dependent mammalian cells onto scaffolds, or transplanting cells in vivo. The cell expansion currently requires repeated cell detachment from solid substrata by enzymatic, chemical or mechanical means. The report here presents a high yield three-dimensional cult...

journal_title：Journal of biotechnology

authors： Chia SM,Lin PC,Quek CH,Yin C,Mao HQ,Leong KW,Xu X,Goh CH,Ng ML,Yu H

更新日期：2005-09-10 00:00:00

abstract：:A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was furthe...

journal_title：Journal of biotechnology

authors： Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RC

更新日期：2000-07-14 00:00:00

abstract：:Saccharum spontaneum L., a wild relative of sugarcane, is known for its adaptability to environmental stresses, particularly cold stress. In the present study, an attempt was made for transcriptome profiling of the low temperature (10°C) tolerant S. spontaneum clone IND 00-1037 collected from high altitude regions of ...

journal_title：Journal of biotechnology

authors： Dharshini S,Chakravarthi M,J AN,Manoj VM,Naveenarani M,Kumar R,Meena M,Ram B,Appunu C

更新日期：2016-08-10 00:00:00

abstract：:Inoculation of naked DNA has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. To study the humoral immune response induced by DNA vaccination we cloned the entire nucleocapsid protein-encoding sequence of the Puumala hantavirus strain Vranica/Hällnäs into th...

journal_title：Journal of biotechnology

authors： Koletzki D,Schirmbeck R,Lundkvist A,Meisel H,Krüger DH,Ulrich R

更新日期：2001-11-17 00:00:00

abstract：:In the present investigation, a detailed biochemical analysis of the high H₂ producer D1 protein mutant strain L159I-N230Y of Chlamydomonas reinhardtii, carrying a double amino acid substitution, was made. The leucine residue L159 was replaced by isoleucine, and the N230 asparagine was replaced by tyrosine. The perfor...

journal_title：Journal of biotechnology

authors： Scoma A,Krawietz D,Faraloni C,Giannelli L,Happe T,Torzillo G

更新日期：2012-02-20 00:00:00

abstract：:Myostatin (MSTN), a member of transforming growth factor-beta superfamily is a negative regulator of the skeletal muscle growth. It suppresses the proliferation and differentiation of myoblast cells. Dysfunction of MSTN gene either by natural mutation or induced through genetic manipulation (knockout or knockdown) has...

journal_title：Journal of biotechnology

authors： Jain H,Singh S,Kadam M,Sarkhel BC

更新日期：2010-01-15 00:00:00

abstract：:Herbinix hemicellulosilytica is a newly isolated, gram-positive, anaerobic bacterium with extensive hemicellulose-degrading capabilities obtained from a thermophilic biogas reactor. In order to exploit its potential as a source for new industrial arabinoxylan-degrading enzymes, six new thermophilic xylanases, four fro...

journal_title：Journal of biotechnology

authors： Mechelke M,Koeck DE,Broeker J,Roessler B,Krabichler F,Schwarz WH,Zverlov VV,Liebl W

更新日期：2017-09-10 00:00:00

abstract：:Consumers prefer biotechnological food products with high nutritional values and good flavors. Solid-state fermentation is a commonly used technique with a long history. In the present study, Myroides sp. ZB35 was used in solid-state fermentative production of aroma volatiles on a rice medium. Using the headspace soli...

journal_title：Journal of biotechnology

authors： Xiao Z,Liang W,Zhu X,Zhao JY

更新日期：2017-03-10 00:00:00

abstract：:Verapamil has been shown to inhibit glucose transport in several cell types. However, the consequences of this inhibition on central metabolism are not well known. In this study we focused on verapamil induced changes in metabolic fluxes in a murine atrial cell line (HL-1 cells). These cells were adapted to serum free...

journal_title：Journal of biotechnology

authors： Strigun A,Noor F,Pironti A,Niklas J,Yang TH,Heinzle E

更新日期：2011-09-20 00:00:00

abstract：:The study was conducted to investigate the effects of foliar-intervention of nano-TiO2 on Cd toxicity in cowpea plants. Cowpea plants were exposed to Cd toxicity at 10 mg/kg soil for 21 days and afterwards, subjected to six episodes of foliar application of nano-TiO2 intervention. Results showed that foliar-applied na...

journal_title：Journal of biotechnology

authors： Ogunkunle CO,Odulaja DA,Akande FO,Varun M,Vishwakarma V,Fatoba PO

更新日期：2020-02-20 00:00:00

abstract：:Six bacterial strains with the capability of degrading textile dyes were isolated from sludge samples and mud lakes. Aeromonas hydrophila was selected and identified because it exhibited the greatest color removal from various dyes. Although A. hydrophila displayed good growth in aerobic or agitation culture (AGI cult...

journal_title：Journal of biotechnology

authors： Chen KC,Wu JY,Liou DJ,Hwang SC

更新日期：2003-02-27 00:00:00

abstract：:The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

journal_title：Journal of biotechnology

authors： Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

更新日期：2014-12-20 00:00:00

abstract：:Amatoxins are ribosomally synthesized and post-translationally modified peptides (RiPPs) found in poisonous mushrooms. These cyclic peptides are potent inhibitors of RNA polymerase II transcriptional activity. Though the macrocyclization of amatoxin is extensively studied, little is known about its subsequent post-tra...

journal_title：Journal of biotechnology

authors： Pathiraja D,Byun J,Lee S,Choi IG

更新日期：2020-01-20 00:00:00

abstract：:The alpha-amylase (1, 4-alpha-d-glucanohydrolase; EC 3.2.1.1) and alpha-glucosidase (alpha-d-glucoside glucohydrolase; EC 3.2.1.20) secreted by Geobacillus thermodenitrificans HRO10 were purified to homogeneity (13.6-fold; 11.5% yield and 25.4-fold; 32.0% yield, respectively) through a series of steps. The molecular w...

journal_title：Journal of biotechnology

authors： Ezeji TC,Bahl H

更新日期：2006-08-20 00:00:00

abstract：:This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene ex...

journal_title：Journal of biotechnology

authors： Sproat BS

更新日期：1995-07-31 00:00:00

abstract：:A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

journal_title：Journal of biotechnology

authors： Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

更新日期：2000-06-23 00:00:00

abstract：:A continuous enzymatic process for the production of the prebiotic disaccharide lactulose through transgalactosylation was developed using free and immobilized beta-glycosidase from Pyrococcus furiosus. The hyperthermostable beta-glycosidase (CelB) was immobilized onto an anion-exchange resin (Amberlite IRA-93) or ont...

journal_title：Journal of biotechnology

authors： Mayer J,Kranz B,Fischer L

更新日期：2010-02-15 00:00:00

abstract：:Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

journal_title：Journal of biotechnology

authors： Chen C,Huang YL,Yang ST

更新日期：2002-07-17 00:00:00

abstract：:The synthesis of galactooligosaccharides (GOS) by whole cells of Bifidobacterium bifidum NCIMB 41171 was investigated by developing a set of mathematical models. These were second order polynomial equations, which described responses related to the production of GOS constituents, the selectivity of lactose conversion ...

journal_title：Journal of biotechnology

authors： Osman A,Tzortzis G,Rastall RA,Charalampopoulos D

更新日期：2010-10-01 00:00:00

abstract：:To improve the specific activity and catalytic efficiency of l-LcLDH1, an NADH-dependent allosteric l-lactate dehydrogenase from L. casei, towards phenylpyruvic acid (PPA), its directed modification was conducted based on the semi-rational design. The three variant genes, Lcldh1Q88R, Lcldh1I229A and Lcldh1T235G, were ...

journal_title：Journal of biotechnology

authors： Li JF,Li XQ,Liu Y,Yuan FJ,Zhang T,Wu MC,Zhang JR

更新日期：2018-09-10 00:00:00

abstract：:Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific propert...

journal_title：Journal of biotechnology

authors： Samoylova TI,Cochran AM,Samoylov AM,Schemera B,Breiteneicher AH,Ditchkoff SS,Petrenko VA,Cox NR

更新日期：2012-12-31 00:00:00

abstract：:The quantitative description of the relationships between global properties, defined at the cellular population level, and individual properties, defined at the single cell level, is considered in this communication along with the analysis of some segregated models of yeast and hybridoma cell cultures. ...

journal_title：Journal of biotechnology

authors： Cazzador L

更新日期：1999-05-28 00:00:00

abstract：:Osmoregulation, the adaptation of cells to changes in the external osmolarity, is an important aspect of the bacterial stress response, in particular for a soil bacterium like Corynebacterium glutamicum. Consequently, this organism is equipped with several redundant systems for coping with both hyper- and hypoosmotic ...

journal_title：Journal of biotechnology

authors： Morbach S,Krämer R

更新日期：2003-09-04 00:00:00

abstract：:5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degree...

journal_title：Journal of biotechnology

authors： Hori N,Watanabe M,Sunagawa K,Uehara K,Mikami Y

更新日期：1991-02-01 00:00:00

abstract：:A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through...

journal_title：Journal of biotechnology

authors： Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Y

更新日期：2006-05-29 00:00:00

abstract：:The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacter...

journal_title：Journal of biotechnology

authors： Tan IKP,Foong CP,Tan HT,Lim H,Zain NA,Tan YC,Hoh CC,Sudesh K

更新日期：2020-04-10 00:00:00