当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Comparative characterization of a recombinant Volvariella volvacea endoglucanase I (EG1) with its truncated catalytic core (EG1-CM), and their impact on the bio-treatment of cellulose-based fabrics.

Comparative characterization of a recombinant Volvariella volvacea endoglucanase I (EG1) with its truncated catalytic core (EG1-CM), and their impact on the bio-treatment of cellulose-based fabrics.

Abstract：

:Recombinant Volvariella volvacea endoglucanase 1 (EG1) and its catalytic module (EG1-CM) were obtained by expression in Pichia pastoris, purified by two-step chromatography, and the catalytic activities and binding capacities were compared. EG1 and EG1-CM exhibited very similar specific activities towards the soluble substrates carboxymethyl cellulose, lichenan and mannan, and insoluble H(3)PO(4) acid-swollen cellulose, whereas the specific activities of EG1-CM towards the insoluble substrates alpha-cellulose, Avicel and filter paper were approximately 58, 43 and 38%, respectively compared to EG1. No increase in reducing sugar release was detected in the reaction mixture supernatants after 50h exposure of filter paper, Avicel or alpha-cellulose to EG1-CM, whereas increases in the total reducing sugar equivalents (i.e. reducing sugar released into solution together with new reducing ends generated in the cellulosic substrates) in reaction mixtures were observed after 1h. In reaction mixtures containing EG1, soluble reducing sugar equivalents were detected in supernatants after 3h incubation with the insoluble cellulosic substrates. EG1-CM did not adsorb to Avicel, and the binding capacities of EG1-CM towards filter paper and H(3)PO(4) acid-swollen cellulose were 27.9-33.3% and 29.6-60.6%, respectively of values obtained with EG1 within the range of total added protein. In enzymatic deinking experiments, the ink removal rate in EG1-CM-treated samples was only slightly higher (approximately 8%), than that of untreated controls, whereas that of the EG1-treated samples was 100% higher. Bio-stoning of denim with EG1-CM resulted in increases of 48% and 40% in weight loss and indigo dye removal, respectively compared with untreated controls. These increases were considerably lower than the corresponding values of 219% and 133% obtained when samples were treated with EG1.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Wu S,Ding S,Zhou R,Li Z

doi

10.1016/j.jbiotec.2007.05.016

subject

Has Abstract

pub_date

2007-07-15 00:00:00

pages

364-9

issue

4

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(07)00367-7

journal_volume

130

pub_type

杂志文章

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