Triggering the production of the cryptic blue pigment indigoidine from Photorhabdus luminescens.

abstract：:Heparan sulfate (HS) is a cell surface carbohydrate polymer modified with sulfate moieties whose highly ordered composition is central to directing specific cell signaling events. The ability of the cell to generate these information rich glycans with such specificity has opened up a new field of "heparanomics" which ...

journal_title：Journal of biotechnology

authors： Lamanna WC,Kalus I,Padva M,Baldwin RJ,Merry CL,Dierks T

更新日期：2007-04-30 00:00:00

abstract：:ACC deaminase producing Plant growth promoting rhizobacteria (PGPR) offers a great promise for ameliorating the negative impacts of salinity stress manifested on plants. In this context, 28 rhizospheric bacteria associated with ACC deaminase potential (198-1069 nmol α-ketobutyrate mg protein-1 h-1) were isolated from ...

journal_title：Journal of biotechnology

authors： Pandey S,Gupta S

更新日期：2020-12-20 00:00:00

abstract：:Pure wine yeast cultures are increasingly used in winemaking to perform controlled fermentations and produce wine of reproducible quality. For the genetic manipulation of natural wine yeast strains dominant selective markers are obviously useful. Here we demonstrate the successful use of the mutated PDR3 gene as a dom...

journal_title：Journal of biotechnology

authors： Kozovska Z,Maraz A,Magyar I,Subik J

更新日期：2001-12-14 00:00:00

abstract：:In this work it is describe for the first time, the use of an esterase with null activity (Tfu_0883 bacterial cutinase from Thermobifida fusca) on the removal of fat from the surface of a cotton substrate. Similar levels of fat removal were found for both null and wild-type proteins, despite that only wild type protei...

journal_title：Journal of biotechnology

authors： Silva C,Azoia NG,Martins M,Matamá T,Wu J,Cavaco-Paulo A

更新日期：2012-10-31 00:00:00

abstract：:Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency o...

journal_title：Journal of biotechnology

authors： Charette SJ,Cornillon S,Cosson P

更新日期：2006-03-09 00:00:00

abstract：:Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based ...

journal_title：Journal of biotechnology

authors： Luna GM,Dell'Anno A,Pietrangeli B,Danovaro R

更新日期：2012-02-20 00:00:00

abstract：:Physiologically relevant in vitro models can serve as biological analytical platforms for testing novel treatments and drug delivery systems. We describe the first steps in the development of a 3D human brain tumour co-culture model that includes the interplay between normal and tumour tissue along with nutrient gradi...

journal_title：Journal of biotechnology

authors： Ivanov DP,Parker TL,Walker DA,Alexander C,Ashford MB,Gellert PR,Garnett MC

更新日期：2015-07-10 00:00:00

abstract：:Bacterial pigments are potential substitute of chemical photosensitizer for dye-sensitized solar cell (DSSC) due to its non-toxic property and cost-effective production from microbial fermentation. Serratia nematodiphila YO1 was isolated from waterfall in Malaysia and identified using 16S ribosomal RNA. Characterizati...

journal_title：Journal of biotechnology

authors： Abdul Manas NH,Chong LY,Tesfamariam YM,Zulkharnain A,Mahmud H,Abang Mahmod DS,Mohamad Fuzi SFZ,Wan Azelee NI

更新日期：2020-06-20 00:00:00

abstract：:Turbulent flows in a single-stage and a two-stage impeller-stirred photobioreactor with a simple geometric configuration were analyzed using computational fluid dynamics. The trajectories of the microorganisms entrained in the flow field were traced by the particle tracking method. By projecting these trajectories ont...

journal_title：Journal of biotechnology

authors： Zhang T

更新日期：2013-10-10 00:00:00

abstract：:Because of their ecological functions, natural products have been optimized in evolution for interaction with biological systems and receptors. However, they have not necessarily been optimized for other desirable drug properties and thus can often be improved by structural modification. Using examples from the litera...

journal_title：Journal of biotechnology

authors： Floss HG

更新日期：2006-06-25 00:00:00

abstract：:Stem borer resistant transgenic parental lines, involved in hybrid rice, were produced by Agrobacterium-mediated gene transfer method. Two pSB111 super-binary vectors containing modified cry1Ab/cry1Ac genes driven by maize ubiquitin promoter, and herbicide resistance gene bar driven by cauliflower mosaic virus 35S pro...

journal_title：Journal of biotechnology

authors： Ramesh S,Nagadhara D,Pasalu IC,Kumari AP,Sarma NP,Reddy VD,Rao KV

更新日期：2004-07-15 00:00:00

abstract：:A gene circuit for the controlled expression of a marker gene and for the assay of xylose concentration in Escherichia coli has been designed and tested. The xylF coding sequence for the xylose binding protein (XBP) was cloned in pT7T318U downstream from the promoter for xylanase A from B. subtilis (Pbsu), together wi...

journal_title：Journal of biotechnology

authors： Ribeiro LF,Bressan F,Furtado GP,Meireles F,Ward RJ

更新日期：2013-12-01 00:00:00

abstract：:Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2...

journal_title：Journal of biotechnology

authors： Dalal M,Tayal D,Chinnusamy V,Bansal KC

更新日期：2009-01-15 00:00:00

abstract：:This paper introduces a novel approach to enhance the strains' biodegradation of 4-chloronitrobenzene by utilizing the synergistic effect of the organic reductant mannitol and the substrate beef extraction. Our results demonstrate that 4-chloronitrobenzene could not be an available nitrogen source to support target st...

journal_title：Journal of biotechnology

authors： Li T,Zhang TC,He L

更新日期：2017-12-20 00:00:00

abstract：:Widely used inactivated vaccines for foot-and-mouth disease (FMD) induce protective immunity, but vaccine production plants and residual virus in the vaccine itself have been implicated in disease outbreaks. The structure of the FMD virion has been determined, and although much of the surface of the viral particle is ...

journal_title：Journal of biotechnology

authors： McKenna TS,Rieder E,Lubroth J,Burrage T,Baxt B,Mason PW

更新日期：1996-01-26 00:00:00

abstract：:The global occurrence of prostate cancer with a range of patient outcome has prompted various investigators to explore novel molecular biomarkers that can precisely detect and track this type of cancer severity. Several studies suggest that micro-RNAs have emerged to act as a new largely unexplored class of biomarkers...

journal_title：Journal of biotechnology

authors： Wani S,Kaul D,Mavuduru RS,Kakkar N,Bhatia A

更新日期：2017-10-10 00:00:00

abstract：:Protein secretion from Bacillus species is a major industrial production tool with a market of over $1 billion per year. However, standard export technologies, based on the well-characterised general secretory (Sec) pathway, are frequently inapplicable for the production of proteins. The recently discovered twin-argin...

journal_title：Journal of biotechnology

authors： van Dijl JM,Braun PG,Robinson C,Quax WJ,Antelmann H,Hecker M,Müller J,Tjalsma H,Bron S,Jongbloed JD

更新日期：2002-09-25 00:00:00

abstract：:Escherichia coli overexpressing a NAD(+)-dependent formate dehydrogenase (FDH) from Candida boidinii was grown in chemostat culture on various carbon sources at 0.05 h(-1) dilution rate, under anaerobic conditions using defined medium and compared to a control without the heterologous FDH pathway. Metabolic fluxes, NA...

journal_title：Journal of biotechnology

authors： Sánchez AM,Bennett GN,San KY

更新日期：2005-06-29 00:00:00

abstract：:The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

journal_title：Journal of biotechnology

authors： Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

更新日期：2014-12-20 00:00:00

abstract：:It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

journal_title：Journal of biotechnology

authors： Hatzis C,Porro D

更新日期：2006-07-13 00:00:00

abstract：:Supervision of batch bioprocess operations in real-time during the progress of a batch run offers many advantages over end-of-batch quality control. Multivariate statistical techniques such as multiway partial least squares (MPLS) provide an efficient modeling and supervision framework. A new type of MPLS modeling tec...

journal_title：Journal of biotechnology

authors： Undey C,Tatara E,Cinar A

更新日期：2004-02-19 00:00:00

abstract：:Protein modifications by intricate cellular machineries often redesign the structure and function of existing proteins to impact biological networks. Disulfide bond formation between cysteine (Cys) pairs is one of the most common modifications found in extracellularly-destined proteins, key to maintaining protein stru...

journal_title：Journal of biotechnology

authors： Zhong X,He T,Prashad AS,Wang W,Cohen J,Ferguson D,Tam AS,Sousa E,Lin L,Tchistiakova L,Gatto S,D'Antona A,Luan YT,Ma W,Zollner R,Zhou J,Arve B,Somers W,Kriz R

更新日期：2017-04-20 00:00:00

abstract：:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

journal_title：Journal of biotechnology

authors： Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

更新日期：1995-02-21 00:00:00

abstract：:This study addresses the problem of poor expression of somatotropin (ST) gene in E. coli and describes expression enhancement through silent and non-silent gene modifications. A series of constructs with codon optimization, substitution, deletion or addition in the 5'-region of the sequence encoding bubaline ST (BbST)...

journal_title：Journal of biotechnology

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2008-06-01 00:00:00

abstract：:Propenylbenzenes are often used as starting materials in the chemical synthesis of aroma compounds and fine chemicals. In the present study, we demonstrate the ability of an Arthrobacter sp. to transform various structures of propenylbenzenes derived from essential oils to flavor, fragrance, and fine chemicals. Arthro...

journal_title：Journal of biotechnology

authors： Shimoni E,Baasov T,Ravid U,Shoham Y

更新日期：2003-10-09 00:00:00

abstract：:Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...

journal_title：Journal of biotechnology

authors： Szostak MP,Hensel A,Eko FO,Klein R,Auer T,Mader H,Haslberger A,Bunka S,Wanner G,Lubitz W

更新日期：1996-01-26 00:00:00

abstract：:A reagentless carbon paste electrode chemically modified with covalently bound alcohol oxidase and horse-radish peroxidase was examined as a selective sensor in flow injection and column liquid chromatography. A combination of carbodiimide, glutaraldehyde, and polyethyleneimine was used for immobilizing the enzymes in...

journal_title：Journal of biotechnology

authors： Johansson K,Jönsson-Pettersson G,Gorton L,Marko-Varga G,Csöregi E

更新日期：1993-12-01 00:00:00

abstract：:Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign protein in lieu of the polyhedrin and hence do not form occlusion bodies (occ-), resulting in ext...

journal_title：Journal of biotechnology

authors： López MG,Alfonso V,Carrillo E,Taboga O

更新日期：2010-01-15 00:00:00

abstract：:The study describes extraction of extracellular polymeric substances (EPS) from sewage sludge by applying enzymes and enzymes combined with sodium tripolyphosphate (STPP). Additionally, a systematic study of two non-enzymatic extraction agents is described. The assessment of the released products is made by colorimetr...

journal_title：Journal of biotechnology

authors： Wawrzynczyk J,Szewczyk E,Norrlöw O,Dey ES

更新日期：2007-06-30 00:00:00

abstract：:The production of cephalosporin C (CPC) and its precursors penicillin N (PEN N), deacetoxycephalosporin C (DAOC) and deacetylcephalosporin C (DAC), with a highly productive strain of Cephalosporin acremonium, was investigated in an 80-1 airlift tower loop reactor with four static mixer modules (Type SMV, Sulzer) (ATLR...

journal_title：Journal of biotechnology

authors： Zhou W,Holzhauer-Rieger K,Bayer T,Schügerl K

更新日期：1993-04-01 00:00:00