Abstract：
:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine by glutamate. Then, we used two chemical engineering methods to eliminate accumulated ammonia. In one the used medium was passed through a natural cation exchanger: the clinoptilolite. In the other, the culture medium was passed through a hydrophobic microporous hollow fiber module. Replacing the glutamine by glutamate reduced the medium ammonia concentration. The physicochemical removal of ammonia induced a better cell growth, but not a better specific antibody secretion.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet Pdoi
10.1016/0168-1656(94)00142-ysubject
Has Abstractpub_date
1995-02-21 00:00:00pages
49-58issue
1eissn
0168-1656issn
1873-4863pii
016816569400142Yjournal_volume
39pub_type
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pub_type： 杂志文章
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