Complete genome sequence of Bacillus thuringiensis YBT-1518, a typical strain with high toxicity to nematodes.

abstract：:The production of a mutant green fluorescent protein (S65TGFP), controlled by the maltose inducible glucoamylase promoter, was followed in situ in fed-batch cultures of recombinant Aspergillus niger using multi-wavelength fluorescence spectroscopy. Disturbance of quantitative product analysis by interfering fluorescen...

journal_title：Journal of biotechnology

authors： Ganzlin M,Marose S,Lu X,Hitzmann B,Scheper T,Rinas U

更新日期：2007-12-01 00:00:00

abstract：:Widely used inactivated vaccines for foot-and-mouth disease (FMD) induce protective immunity, but vaccine production plants and residual virus in the vaccine itself have been implicated in disease outbreaks. The structure of the FMD virion has been determined, and although much of the surface of the viral particle is ...

journal_title：Journal of biotechnology

authors： McKenna TS,Rieder E,Lubroth J,Burrage T,Baxt B,Mason PW

更新日期：1996-01-26 00:00:00

abstract：:A striking property of many prokaryotes is their enormous metabolic flexibility with respect not only to catabolic and anabolic substrates but also with respect to the continuously changing availability of nutrients. The phenotypic responses to low-nutrient growth conditions involve structural changes in the cellular ...

journal_title：Journal of biotechnology

authors： Teixeira de Mattos MJ,Neijssel OM

更新日期：1997-12-17 00:00:00

abstract：:The unsaturations and groups bound to the ring and to the lateral chain of lactones give a large diversity in this class of molecules. In this work we produced enzymatically a macrolactone in organic media. The substrate used was a hydroxy-fatty acid: (+)-coriolic acid and the enzymes tested were free or immobilized m...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2002-01-18 00:00:00

abstract：:The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30...

journal_title：Journal of biotechnology

authors： Ouyang J,Wang X,Zhao B,Yuan X,Wang Y

更新日期：2003-04-24 00:00:00

abstract：:There are many examples of enzymes that share substrates or cofactors in a cyclic manner. Techniques have been developed that use cyclic enzyme systems to assay quantitatively small amounts of biochemical substances (cofactor, substrate), however, only a few studies of the control of these systems have been published....

journal_title：Journal of biotechnology

authors： Okamoto M

更新日期：1992-06-01 00:00:00

abstract：:One hundred and seventy-eight new butanol-acetone producing bacteria related to saccharolytic clostridia were isolated from agricultural sources in Colombia and their fermentation potential was evaluated. Thirteen isolates produced more total solvents from glucose than Clostridium acetobutylicum ATCC 824. The isolates...

journal_title：Journal of biotechnology

authors： Montoya D,Spitia S,Silva E,Schwarz WH

更新日期：2000-04-28 00:00:00

abstract：:In this work it is describe for the first time, the use of an esterase with null activity (Tfu_0883 bacterial cutinase from Thermobifida fusca) on the removal of fat from the surface of a cotton substrate. Similar levels of fat removal were found for both null and wild-type proteins, despite that only wild type protei...

journal_title：Journal of biotechnology

authors： Silva C,Azoia NG,Martins M,Matamá T,Wu J,Cavaco-Paulo A

更新日期：2012-10-31 00:00:00

abstract：:Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

journal_title：Journal of biotechnology

authors： Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

更新日期：2001-10-04 00:00:00

abstract：:Enteric infections associated with bacteria, viruses and parasites are a world health problem accounting for at least one billion episodes of diarrhoea and up to 10 million deaths in the Third World each year. The widely held conclusion that responses which are protective in the gastrointestinal tract are best stimula...

journal_title：Journal of biotechnology

authors： Stokes CR,Bailey M

更新日期：1996-01-26 00:00:00

abstract：:The production of isoprenoids in recombinant microbes for flavor & fragrance, pharmaceutical, agricultural or fuel applications is a booming research field. Isoprenoid extraction from natural resources and chemical synthesis is frequently neither ecological nor commercially profitable. However, recombinant microbes al...

journal_title：Journal of biotechnology

authors： Emmerstorfer-Augustin A,Moser S,Pichler H

更新日期：2016-10-10 00:00:00

abstract：:The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two clones of belladonna hairy roots were studied. The effects of nitrate and ammonium concentrations on these cultures were investigated. A rise in ammonium concentration caused the decline of the hairy roots, while nitrate had a mar...

journal_title：Journal of biotechnology

authors： Bensaddek L,Gillet F,Saucedo JE,Fliniaux MA

更新日期：2001-01-23 00:00:00

abstract：:Flow cytometry was used to demonstrate the presence of beta-lactoglobulin (betaLG) receptors on living murine hybridoma MARK-3 cells using a fluorescein isothiocyanate-betaLG conjugate (FITC-betaLG: molar ratio of 5:1). A site occupation curve was produced using a shift in the mean channel fluorescence at various conc...

journal_title：Journal of biotechnology

authors： Palupi NS,Franck P,Guimont C,Linden G,Dumas D,Stoltz J,Nabet P,Belleville-Nabet F,Dousset B

更新日期：2000-03-10 00:00:00

abstract：:The synthesis of methyl alpha-D-glucooligosaccharides, using sucrose as glucosyl donor and methyl alpha-D-glucopyranoside as acceptor, was studied with dextransucrase from Leuconostoc mesenteroides NRRL B-1299. The enzyme was immobilized by entrapment in alginate. By NMR and mass spectrometry we identified three homol...

journal_title：Journal of biotechnology

authors： de Segura AG,Alcalde M,Bernabé M,Ballesteros A,Plou FJ

更新日期：2006-07-13 00:00:00

abstract：:The hematopoietic system represents an interesting model for gene transfer protocols. Here, we have evaluated the efficiency of a gene transfer system using the polycationic compound SuperFect (Qiagen) and the K562 hematopoietic cell line. Transient and stable vectors carrying the enhanced green fluorescent protein (E...

journal_title：Journal of biotechnology

authors： Teixeira LA,Fricke CH,Bonorino CB,Bogo MR,Nardi NB

更新日期：2001-06-15 00:00:00

abstract：:The combination of anaerobic and aerobic periods in the operation cycle of a Sequencing Batch Reactor (SBR) was chosen to study biological color removal from simulated textile effluents containing reactive, sulfonated, monoazo and diazo dyes, respectively, Remazol Brilliant Violet 5R and Remazol Black B. 90% color rem...

journal_title：Journal of biotechnology

authors： Lourenço ND,Novais JM,Pinheiro HM

更新日期：2001-08-23 00:00:00

abstract：:A DNA construct containing the human alpha 1-antitrypsin gene including 1.5 and 4 kb of 5' and 3' flanking sequences, was microinjected into the pronucleus of rabbit embryos. The recombinant human protein was (a) expressed in the blood circulation of F0 and F1 transgenic rabbits at an average concentration of 1 mg ml-...

journal_title：Journal of biotechnology

authors： Massoud M,Bischoff R,Dalemans W,Pointu H,Attal J,Schultz H,Clesse D,Stinnakre MG,Pavirani A,Houdebine LM

更新日期：1991-05-01 00:00:00

abstract：:This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible,...

journal_title：Journal of biotechnology

authors： Franck P,Petitipain N,Cherlet M,Dardennes M,Maachi F,Schutz B,Poisson L,Nabet P

更新日期：1996-05-15 00:00:00

abstract：:Baculovirus vectors show promise as a novel tool for gene delivery into mammalian cells and gene transfer with wild-type baculovirus has been demonstrated both in vitro and in vivo. To study expression and intracellular trafficking of foreign viral membrane proteins in baculovirus-transduced mammalian cells, the envel...

journal_title：Journal of biotechnology

authors： Ojala K,Tikka PJ,Kautto L,Käpylä P,Marjomäki V,Oker-Blom C

更新日期：2004-10-19 00:00:00

abstract：:Enzymatic synthesis of acylated derivatives of a monosaccharidic flavonoid chrysoeriol-7-O-beta-D-(3''-E-p-coumaroyl)-glucopyranoside as well as of a disaccharidic flavonoid chrysoeriol-7-[6'''-O-acetyl-beta-D-allosyl-(1-->2)-beta-D-glucopyranoside], isolated from Greek endemic plants, was performed using an immobiliz...

journal_title：Journal of biotechnology

authors： Mellou F,Lazari D,Skaltsa H,Tselepis AD,Kolisis FN,Stamatis H

更新日期：2005-03-30 00:00:00

abstract：:Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 microm) of macroporous poly(dimethylacrylamide) makes it possible to couple l...

journal_title：Journal of biotechnology

authors： Noppe W,Plieva FM,Vanhoorelbeke K,Deckmyn H,Tuncel M,Tuncel A,Galaev IY,Mattiasson B

更新日期：2007-09-15 00:00:00

abstract：:Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

journal_title：Journal of biotechnology

authors： Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

更新日期：2005-03-02 00:00:00

abstract：:Escherichia coli secreted protein A (EspA) is a component of the type 3 secretion system (T3SS). The high level of expression when self-stimulated suggests that EspA may be used as a fusion partner. In the present study, EspA was used as a "fusion partner" to construct a fusion expression system, pEspA, in order to im...

journal_title：Journal of biotechnology

authors： Cheng Y,Gu J,Wang HG,Yu S,Liu YQ,Ning YL,Zou QM,Yu XJ,Mao XH

更新日期：2010-11-01 00:00:00

abstract：:Tissue engineering involves ex vivo seeding of anchorage-dependent mammalian cells onto scaffolds, or transplanting cells in vivo. The cell expansion currently requires repeated cell detachment from solid substrata by enzymatic, chemical or mechanical means. The report here presents a high yield three-dimensional cult...

journal_title：Journal of biotechnology

authors： Chia SM,Lin PC,Quek CH,Yin C,Mao HQ,Leong KW,Xu X,Goh CH,Ng ML,Yu H

更新日期：2005-09-10 00:00:00

abstract：:Threonine aldolase (TA) catalyzes a reversible reaction, in which threonine is decomposed into glycine and acetaldehyde. The same enzyme can be used to catalyze aldol reaction between glycine and a variety of aromatic and aliphatic aldehydes, thus creating various alpha-amino-alcohols. Therefore, TA is a very promisin...

journal_title：Journal of biotechnology

authors： Wieteska L,Ionov M,Szemraj J,Feller C,Kolinski A,Gront D

更新日期：2015-04-10 00:00:00

abstract：:5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degree...

journal_title：Journal of biotechnology

authors： Hori N,Watanabe M,Sunagawa K,Uehara K,Mikami Y

更新日期：1991-02-01 00:00:00

abstract：:The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared ...

journal_title：Journal of biotechnology

authors： Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer J

更新日期：2000-09-29 00:00:00

abstract：:A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through...

journal_title：Journal of biotechnology

authors： Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Y

更新日期：2006-05-29 00:00:00

abstract：BACKGROUND:In recent years, several mass spectrometry-based omics technologies emerged to investigate qualitative and quantitative changes within thousands of biologically active components such as proteins, lipids and metabolites. The research enabled through these methods potentially contributes to the diagnosis and ...

journal_title：Journal of biotechnology

authors： Pfeuffer J,Sachsenberg T,Alka O,Walzer M,Fillbrunn A,Nilse L,Schilling O,Reinert K,Kohlbacher O

更新日期：2017-11-10 00:00:00

abstract：:In order to develop a serum-free (SF) medium for the production of erythropoietin (EPO) by suspension culture of recombinant Chinese hamster ovary (rCHO) cells, a statistical optimization approach based on a Plackett-Burman design was adopted. A basal medium was prepared by supplementing Iscove's modified Dulbecco's m...

journal_title：Journal of biotechnology

authors： Lee GM,Kim EJ,Kim NS,Yoon SK,Ahn YH,Song JY

更新日期：1999-04-15 00:00:00