Abstract：
:The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two clones of belladonna hairy roots were studied. The effects of nitrate and ammonium concentrations on these cultures were investigated. A rise in ammonium concentration caused the decline of the hairy roots, while nitrate had a marked effect on the alkaloid content. The alkaloid production obtained with 15.8 mM of NO3- and 20.5 mM of NH4+ was 1.2-1.4 times higher than that obtained when the roots were grown in the standard Murashige and Skoog medium (MS medium, 39.5 mM of NO3- and 20.5 mM of NH4+). The nitrate and ammonium concentrations in the culture medium also had a strong influence on the scopolamine/hyoscyamine ratio. When nitrate or ammonium concentrations were raised, that ratio also was increased 2-3-fold. The hairy root clones originating from transformations with two distinct strains of Agrobacterium had similar responses.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Bensaddek L,Gillet F,Saucedo JE,Fliniaux MAdoi
10.1016/s0168-1656(00)00372-2subject
Has Abstractpub_date
2001-01-23 00:00:00pages
35-40issue
1eissn
0168-1656issn
1873-4863pii
S0168-1656(00)00372-2journal_volume
85pub_type
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