Complete genome sequence of the methanogenic neotype strain Methanobacterium formicicum MF(T.).

abstract：:Plasmid-free and plasmid-harbouring E. coli JM109 strains were investigated in shaken flasks, stirred tanks in batch and continuous operation. The shaken flask cultivations were performed in M9 minimal medium and in media with various protein supplements. The host hardly grows on M9 minimal medium as opposed to the pl...

journal_title：Journal of biotechnology

authors： Rhee JI,Bode J,Diaz-Ricci JC,Poock D,Weigel B,Kretzmer G,Schügler K

更新日期：1997-06-13 00:00:00

abstract：:Myostatin (MSTN), a member of transforming growth factor-beta superfamily is a negative regulator of the skeletal muscle growth. It suppresses the proliferation and differentiation of myoblast cells. Dysfunction of MSTN gene either by natural mutation or induced through genetic manipulation (knockout or knockdown) has...

journal_title：Journal of biotechnology

authors： Jain H,Singh S,Kadam M,Sarkhel BC

更新日期：2010-01-15 00:00:00

abstract：:The efficient separation of Corynebacterium glutamicum cells from culture medium by size exclusion chromatography (SEC) is presented. Residue analysis demonstrated that this method effectively depletes extracellular compounds. For evaluation, SEC was compared with the common methods cold methanol treatment, fast centr...

journal_title：Journal of biotechnology

authors： Persicke M,Plassmeier J,Neuweger H,Rückert C,Pühler A,Kalinowski J

更新日期：2011-07-10 00:00:00

abstract：:Software sensors for monitoring and on-line estimation of critical bioprocess variables have mainly been used with standard bioreactor sensors, such as electrodes and gas analyzers, where algorithms in the software model have generated the desired state variables. In this article we propose that other on-line instrume...

journal_title：Journal of biotechnology

authors： Warth B,Rajkai G,Mandenius CF

更新日期：2010-05-03 00:00:00

abstract：:31P nuclear magnetic resonance (31P NMR) was used to monitor cytoplasmic and vacuolar pH values in the filamentous fungus Aspergillus niger. To obtain a homogeneous cell sample and to be able to perform long term in vivo NMR measurements A. niger mycelium was kept in a setup that allows perfusion of the cell plug with...

journal_title：Journal of biotechnology

authors： Hesse SJ,Ruijter GJ,Dijkema C,Visser J

更新日期：2000-01-28 00:00:00

abstract：:Batch bioreactor cultivations using Saccharomyces cerevisiae at high (190-305 gl(-1) glucose) or low (21-25 gl(-1) glucose) gravity conditions were monitored on-line using multi-wavelength fluorescence (MWF) and standard monitoring sensors. Partial least squares models were calibrated for the prediction of cell dry we...

journal_title：Journal of biotechnology

authors： Odman P,Johansen CL,Olsson L,Gernaey KV,Lantz AE

更新日期：2009-10-26 00:00:00

abstract：:Widely used inactivated vaccines for foot-and-mouth disease (FMD) induce protective immunity, but vaccine production plants and residual virus in the vaccine itself have been implicated in disease outbreaks. The structure of the FMD virion has been determined, and although much of the surface of the viral particle is ...

journal_title：Journal of biotechnology

authors： McKenna TS,Rieder E,Lubroth J,Burrage T,Baxt B,Mason PW

更新日期：1996-01-26 00:00:00

abstract：:Oxygen tension regulates the biological characteristics of hematopoietic stem and progenitor cells (HSPCs) by modulating intracellular reactive oxygen species (ROS). To better understand oxygen tension mechanism on HSPCs culture, gene expression analysis of human CD34(+)CD38(-) HSPCs was performed using microarrays. T...

journal_title：Journal of biotechnology

authors： Fan J,Cai H,Li Q,Du Z,Tan W

更新日期：2012-04-15 00:00:00

abstract：:The haloarchaeon Haloferax mediterranei is able to grow in the presence of different inorganic and organic nitrogen sources by means of the assimilatory pathway under aerobic conditions. In order to identify genes of potential importance in nitrogen metabolism and its regulation in the halophilic microorganism, we hav...

journal_title：Journal of biotechnology

authors： Esclapez J,Pire C,Camacho M,Bautista V,Martínez-Espinosa RM,Zafrilla B,Vegara A,Alcaraz LA,Bonete MJ

更新日期：2015-01-10 00:00:00

abstract：:The first problem in gene expression profiling to be solved is choosing the appropriate gene array, detection procedure, image analysis and data generation depending on the organism of interest, equipment and budget. The next one is how to deduce biologically meaningful data. We assessed gene expression data from chem...

journal_title：Journal of biotechnology

authors： Fahnert B,Hahn D,Guthke R

更新日期：2002-03-14 00:00:00

abstract：:Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. The wild-type strain NCPPB382 carries two plasmids, pCM1 and pCM2. The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no dise...

journal_title：Journal of biotechnology

authors： Gartemann KH,Kirchner O,Engemann J,Gräfen I,Eichenlaub R,Burger A

更新日期：2003-12-19 00:00:00

abstract：:The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30...

journal_title：Journal of biotechnology

authors： Ouyang J,Wang X,Zhao B,Yuan X,Wang Y

更新日期：2003-04-24 00:00:00

abstract：:In earlier study from our group, cholera toxin B subunit had been expressed in tomato for developing a plant-based vaccine against cholera. In the present investigation, gene for accessory colonization factor (acf) subunit A, earlier reported to be essential for efficient colonization in the intestine, has been expres...

journal_title：Journal of biotechnology

authors： Sharma MK,Jani D,Thungapathra M,Gautam JK,Meena LS,Singh Y,Ghosh A,Tyagi AK,Sharma AK

更新日期：2008-05-20 00:00:00

abstract：:The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared ...

journal_title：Journal of biotechnology

authors： Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer J

更新日期：2000-09-29 00:00:00

abstract：:beta-galactosidase reporter plasmids containing different viral or minimal promoters are commonly used to correct variable transfection efficiencies in transient transfection experiments. The transcriptional activity of these promoters is thought to be stable under most circumstances. To determine if expression of bet...

journal_title：Journal of biotechnology

authors： Huszár T,Mucsi I,Terebessy T,Masszi A,Adamkó S,Jeney C,Rosivall L

更新日期：2001-07-12 00:00:00

abstract：:To study the metaproteome of a biogas-producing microbial community, fermentation samples were taken from an agricultural biogas plant for microbial cell and protein extraction and corresponding metagenome analyses. Based on metagenome sequence data, taxonomic community profiling was performed to elucidate the composi...

journal_title：Journal of biotechnology

authors： Ortseifen V,Stolze Y,Maus I,Sczyrba A,Bremges A,Albaum SP,Jaenicke S,Fracowiak J,Pühler A,Schlüter A

更新日期：2016-08-10 00:00:00

abstract：:The study describes extraction of extracellular polymeric substances (EPS) from sewage sludge by applying enzymes and enzymes combined with sodium tripolyphosphate (STPP). Additionally, a systematic study of two non-enzymatic extraction agents is described. The assessment of the released products is made by colorimetr...

journal_title：Journal of biotechnology

authors： Wawrzynczyk J,Szewczyk E,Norrlöw O,Dey ES

更新日期：2007-06-30 00:00:00

abstract：:Peptide synthesis was carried out in a variety of organic solvents with low contents of water. The enzyme was deposited on the support material, celite, from an aqueous buffer solution. After evaporation of the water the biocatalyst was suspended in the reaction mixtures. The chymotrypsin-catalyzed reaction between Z-...

journal_title：Journal of biotechnology

authors： Clapés P,Adlercreutz P,Mattiasson B

更新日期：1990-09-01 00:00:00

abstract：:Extracellular enzymes are assumed to be responsible for the initial and rate limiting step in biodegradation of polymers. Mainly enzymes with aliphatic esters as their natural substrates (e.g. lipase, cutinases) have until now been evaluated for polyester hydrolysis studies. However, the potential of enzymes with arom...

journal_title：Journal of biotechnology

authors： Haernvall K,Zitzenbacher S,Yamamoto M,Schick MB,Ribitsch D,Guebitz GM

更新日期：2017-09-10 00:00:00

abstract：:The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

journal_title：Journal of biotechnology

authors： Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

更新日期：2014-02-20 00:00:00

abstract：:We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch or a gradual adaptation, we have achieved BHK-21 cells growth in the following media: HyQ PF CHO, HyQ PF CHO MPS, Ren...

journal_title：Journal of biotechnology

authors： Kallel H,Jouini A,Majoul S,Rourou S

更新日期：2002-05-23 00:00:00

abstract：:A new β-glucosidase gene (bglSp) was cloned from the ginsenoside converting Sphingomonas sp. strain 2F2 isolated from the ginseng cultivating filed. The bglSp consisted of 1344 bp (447 amino acid residues) with a predicted molecular mass of 49,399 Da. A BLAST search using the bglSp sequence revealed significant homolo...

journal_title：Journal of biotechnology

authors： Wang L,Liu QM,Sung BH,An DS,Lee HG,Kim SG,Kim SC,Lee ST,Im WT

更新日期：2011-11-10 00:00:00

abstract：:Sodium butyrate was used to enhance expression levels and thereby facilitate the generation of analytical quantities of nine different tissue plasminogen activator (tPA) analogues expressed under the control of the cytomegalovirus immediate early (CMV IE) promoter by the Chinese hamster ovary (CHO) mammalian expressio...

journal_title：Journal of biotechnology

authors： Palermo DP,DeGraaf ME,Marotti KR,Rehberg E,Post LE

更新日期：1991-06-01 00:00:00

abstract：:We established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the HiCEP (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. Following the removal of abundant rRNA, the mRNA of prokaryotic cells was enzymatic...

journal_title：Journal of biotechnology

authors： Mitani Y,Suzuki K,Kondo K,Okumura K,Tamura T

更新日期：2006-05-29 00:00:00

abstract：:Bacillus thuringiensis is a ubiquitous spore-forming bacterium and has been widely used as a biopesticide for controlling agricultural insects by the production of insecticidal crystal proteins (ICPs). B. thuringiensis YBT-1518 displays effective toxicity to nematodes. This strain harbors three nematicidal crystal pro...

journal_title：Journal of biotechnology

authors： Wang P,Zhang C,Guo M,Guo S,Zhu Y,Zheng J,Zhu L,Ruan L,Peng D,Sun M

更新日期：2014-02-10 00:00:00

abstract：:Two extra-cellular endoxylanases (Xyl Ia and Ib) were purified to homogeneity from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Xyl Ia and Ib, having a molecular mass of approximately 53 kDa and pI of 5.2 and 4.8, respectively, were optimally active at 75 degrees C and at pH 6.0. They were st...

journal_title：Journal of biotechnology

authors： Chadha BS,Ajay BK,Mellon F,Bhat MK

更新日期：2004-04-29 00:00:00

abstract：:A comparative study was made of Mung bean epoxide hydrolases-catalyzed asymmetric hydrolysis of styrene oxide to (R)-1-phenyl-1,2-ethanediol in an n-hexane/buffer biphasic system containing various hydrophilic ionic liquids (ILs). Compared to the n-hexane/buffer biphasic system alone, addition of a small amount of hyd...

journal_title：Journal of biotechnology

authors： Chen WJ,Lou WY,Yu CY,Wu H,Zong MH,Smith TJ

更新日期：2012-12-31 00:00:00

abstract：:Vitamin D is essential for bone health and has significant roles in non-skeletal health and organ function. Dermal synthesis through exposure to ultraviolet B light is the major natural source of vitamin D, while only a small portion of the necessary amount can be acquired by a diet without fortified foods. In recent ...

journal_title：Journal of biotechnology

authors： Moulas AN,Vaiou M

更新日期：2018-11-10 00:00:00

abstract：:Cannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella p...

journal_title：Journal of biotechnology

authors： Zirpel B,Kayser O,Stehle F

更新日期：2018-10-20 00:00:00

abstract：:As oleaginous microorganisms represent an upcoming novel feedstock for the biotechnological production of lipids or lipid-derived biofuels, we searched for novel, lipid-producing strains in desert soil. This was encouraged by the hypothesis that neutral lipids represent an ideal storage compound, especially under arid...

journal_title：Journal of biotechnology

authors： Röttig A,Hauschild P,Madkour MH,Al-Ansari AM,Almakishah NH,Steinbüchel A

更新日期：2016-05-10 00:00:00