Abstract:
:In concern with frequent recurrence of anthrax in endemic areas and inadvertent use of its spores as biological weapon, the development of an effective anthrax vaccine suitable for both human and veterinary needs is highly desirable. A simple oral delivery through expression in plant system could offer promising alternative to the current methods that rely on injectable vaccines extracted from bacterial sources. In the present study, we have expressed protective antigen (PA) gene in Indian mustard by Agrobacterium-mediated transformation and in tobacco by plastid transformation. Putative transgenic lines were verified for the presence of transgene and its expression by molecular analysis. PA expressed in transgenic lines was biologically active as evidenced by macrophage lysis assay. Intraperitoneal (i.p.) and oral immunization with plant PA in murine model indicated high serum PA specific IgG and IgA antibody titers. PA specific mucosal immune response was noted in orally immunized groups. Further, antibodies indicated lethal toxin neutralizing potential in-vitro and conferred protection against in-vivo toxin challenge. Oral immunization experiments demonstrated generation of immunoprotective response in mice. Thus, our study examines the feasibility of oral PA vaccine expressed in an edible plant system against anthrax.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Gorantala J,Grover S,Rahi A,Chaudhary P,Rajwanshi R,Sarin NB,Bhatnagar Rdoi
10.1016/j.jbiotec.2014.01.033subject
Has Abstractpub_date
2014-04-20 00:00:00pages
1-10eissn
0168-1656issn
1873-4863pii
S0168-1656(14)00057-1journal_volume
176pub_type
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