Abstract:
:Production of heterologous proteins in bacteria is one of the main applications of biotechnology. Although several high-efficiency expression systems have been developed, different steps in protein production may become rate-limiting depending on the production system and the protein being produced. One bottle neck can be the instability of the mRNA. We have used fragments of the unusually long-living mRNA hrpA from the plant pathogenic bacteria Pseudomonas syringae pathovars tomato and phaseolicola to increase the half-lives of heterologous transcripts. The stabilizing effect was extended to Escherichia coli, as half-lives of several heterologous transcripts were increased from a few minutes to up to 19min. Production of heterologous proteins was also increased manifold by the addition of the stabilizing hrpA elements. We have mapped the regions of the hrpA transcript necessary and sufficient for the stabilization process.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Hienonen E,Romantschuk M,Fenel F,Taira Sdoi
10.1016/j.jbiotec.2006.10.010subject
Has Abstractpub_date
2007-02-01 00:00:00pages
258-67issue
2eissn
0168-1656issn
1873-4863pii
S0168-1656(06)00871-6journal_volume
128pub_type
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