Abstract：
:In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver nanoparticles were investigated by UV-vis spectroscopy and Atomic Force Microscopy (AFM). Free amine groups were introduced on the surface of native silver nanoparticles by coating a uniform layer of polyaniline and this was confirmed by FTIR spectroscopy and Scanning Electron Microscopy. Functionalized silver nanoparticles were then grafted to the C3 carboxyl group of the β lactam ring of penicillin G in the presence of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDAC) with a conjugation yield of 213 μg mg⁻¹. These novel silver penicillin G nanoconjugates showed a very good growth inhibition against both non-resistant Escherichia coli (gram negative) as well as toward β lactam resistant, E. coli (gram negative) and Staphylococcus aureus (gram positive).
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NSdoi
10.1016/j.jbiotec.2012.12.002subject
Has Abstractpub_date
2013-02-20 00:00:00pages
419-24issue
4eissn
0168-1656issn
1873-4863pii
S0168-1656(12)00736-5journal_volume
163pub_type
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