Abstract：
:In this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by Aspergillus oryzae (A. oryzae), which was immobilized in plastic composite support (PCS) bioreactor. Nitrogen deficient medium was applied to increase the production of KA in PCS-immobilized bioreactor. The efficiency of immobilized culture for kojic acid (KA) production and the effect of morphology of A. oryzae on KA production were evaluated. After three cycles of cultivation, 83.47 g/L of KA was produced in PCS bioreactor in nitrogen deficient medium with productivity of 3.09 g/L/d, which is higher than free suspension culture in batch fermentation. The morphology of A. oryzae mycelium changed under nitrogen starvation. Feather-like mycelium was observed with increasing KA production. RNA expression (kojA and kojT) results indicated that the nitrogen deficient environment had strong influence on KA production on the transcriptional level. PCS immobilized fermentation system, which allowed a repeated-batch fermentation with higher production and productivity, is a potential tool in industrial production of KA.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Liu JM,Yu TC,Lin SP,Hsu RJ,Hsu KD,Cheng KCdoi
10.1016/j.jbiotec.2015.11.023subject
Has Abstractpub_date
2016-01-20 00:00:00pages
41-8eissn
0168-1656issn
1873-4863pii
S0168-1656(15)30194-2journal_volume
218pub_type
杂志文章abstract：:Bacillus thuringiensis is a ubiquitous spore-forming bacterium and has been widely used as a biopesticide for controlling agricultural insects by the production of insecticidal crystal proteins (ICPs). B. thuringiensis YBT-1518 displays effective toxicity to nematodes. This strain harbors three nematicidal crystal pro...
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journal_title：Journal of biotechnology
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