当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination.

Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination.

Abstract：

:Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency of homologous recombination. Here we present an easy method to identify rare mutants, based on PCR analysis of pools of clones. This method also allows the isolation of functional knockout mutants created by a single homologous recombination event, which can be more frequent than a double recombination event.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Charette SJ,Cornillon S,Cosson P

doi

10.1016/j.jbiotec.2005.08.023

subject

Has Abstract

pub_date

2006-03-09 00:00:00

pages

1-4

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(05)00553-5

journal_volume

122

pub_type

杂志文章

相关文献

JOURNAL OF BIOTECHNOLOGY文献大全
  • Characterization of the promoter and upstream activating sequence from the Pseudomonas alcaligenes lipase gene.

    abstract：:Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00397-7

    authors： Cox M,Gerritse G,Dankmeyer L,Quax WJ

    更新日期：2001-03-09 00:00:00

  • Identification of a novel endogenous regulatory element in Chinese hamster ovary cells by promoter trap.

    abstract：:The objective of this study was to identify and isolate endogenous promoters in Chinese hamster ovary (CHO) cells using a promoter trap approach. A promoter-less vector harboring a green fluorescent protein (GFP)-hygromycin resistance gene cassette was designed and transfected into CHO cells. Putative promoters were i...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.07.001

    authors： Chen J,Haverty J,Deng L,Li G,Qiu P,Liu Z,Shi S

    更新日期：2013-09-10 00:00:00

  • Measurements of sulfur, phosphorus and other ions in microbial biomass: influence on correct determination of elemental composition and degree of reduction.

    abstract：:The elemental composition of microorganisms varies widely with respect to the N, S, P and ash content as well as many trace elements depending on microorganism and growth conditions. As a consequence, the mass of 1 C-mol of biomass can differ considerably from one microorganism to another. Experimental results show th...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(95)00135-0

    authors： Duboc P,Schill N,Menoud L,van Gulik W,von Stockar U

    更新日期：1995-12-01 00:00:00

  • A simple analysis system for the estimation of recombination efficiency using fluorescence-activated cell sorting.

    abstract：:A simple and accurate analysis system for the estimation of recombination efficiency in vivo using fluorescence-activated cell sorting (FACS) was designed and was subsequently used to compare the efficiency of recombination related to different spacer mutants. F(3) and F(5) mutant sequences were used for Flpe-mediated...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.07.027

    authors： Kim MS,Kim WH,Lee GM

    更新日期：2007-01-10 00:00:00

  • Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice.

    abstract：:Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinan...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.01.028

    authors： Minic R,Gavrovic-Jankulovic M,Petrusic V,Zivkovic I,Eijsink VG,Dimitrijevic L,Mathiesen G

    更新日期：2015-04-10 00:00:00

  • Test system for evaluating the influence of polymer properties on primary human keratinocytes and fibroblasts in mono- and coculture.

    abstract：:Interactions of cells with polymer-based biomaterials are influenced by properties of the substrate. Polymers, which are able to induce cell specific effects, gain increasing importance for biotechnology and regenerative therapies. A test system was developed, which allows studying primary human keratinocytes and fibr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.04.012

    authors： Trescher K,Roch T,Cui J,Kratz K,Lendlein A,Jung F

    更新日期：2013-06-20 00:00:00

  • Purification and characterization of a new bioscouring pectate lyase from Bacillus pumilus BK2.

    abstract：:An alkalophilic bacterium was isolated based on the potential of extra-cellular enzymes for bioscouring. The bacterium was identified as a new strain of Bacillus pumilus BK2 producing an extra-cellular endo-pectate lyase PL (EC 4.2.2.2). PL was purified to homogeneity in three steps and has a molecular mass of 37.3+/-...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.07.019

    authors： Klug-Santner BG,Schnitzhofer W,Vrsanská M,Weber J,Agrawal PB,Nierstrasz VA,Guebitz GM

    更新日期：2006-02-10 00:00:00

  • RNA-bioinformatics: Tools, services and databases for the analysis of RNA-based regulation.

    abstract：:The importance of RNA-based regulation is becoming more and more evident. Genome-wide sequencing efforts have shown that the majority of the DNA in eukaryotic genomes is transcribed. Advanced high-throughput techniques like CLIP for the genome-wide detection of RNA-protein interactions have shown that post-transcripti...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jbiotec.2017.05.019

    authors： Backofen R,Engelhardt J,Erxleben A,Fallmann J,Grüning B,Ohler U,Rajewsky N,Stadler PF

    更新日期：2017-11-10 00:00:00

  • Transformation of Bowes melanoma cells with SV40 T antigen.

    abstract：:A serum-dependent and two serum-independent variants of the Bowes melanoma cell line, RPMI7272, were transfected with plasmids containing a geneticin-resistance (neo) gene transcribed by the HSV thymidine kinase promoter and an SV40 T antigen gene under control of the mouse metallothionein I promoter. T-antigen increa...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(92)90014-z

    authors： Asselbergs FA

    更新日期：1992-11-01 00:00:00

  • Screening for improved isoprenoid biosynthesis in microorganisms.

    abstract：:The production of isoprenoids in recombinant microbes for flavor & fragrance, pharmaceutical, agricultural or fuel applications is a booming research field. Isoprenoid extraction from natural resources and chemical synthesis is frequently neither ecological nor commercially profitable. However, recombinant microbes al...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jbiotec.2016.03.051

    authors： Emmerstorfer-Augustin A,Moser S,Pichler H

    更新日期：2016-10-10 00:00:00

  • DNA recovery and PCR quantification of catechol 2,3-dioxygenase genes from different soil types.

    abstract：:With the objective of monitoring xenobiotic degrading bacteria in soil, a method for rapid extraction of DNA from soil, amenable to amplification by PCR, was developed. The method was based on lysis by freeze-thawing and subsequent addition of sodium dodecyl sulfate (SDS), hexadecyltrimethylammonium bromide and protei...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(96)01635-5

    authors： Wikström P,Wiklund A,Andersson AC,Forsman M

    更新日期：1996-12-10 00:00:00

  • Model-based automation of baker's yeast production.

    abstract：:An on-line model, estimating key state variables in bioprocesses, is utilized for control of fed-batch baker's yeast production. The state estimates are produced by balances and phenomenological expressions combined with on-line measurements. The goal of the control strategy is to maintain the highest possible glucose...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(96)01570-2

    authors： Ringbom K,Rothberg A,Saxén B

    更新日期：1996-10-18 00:00:00

  • Solid-phase cloning to create sublibraries suitable for DNA sequencing.

    abstract：:A solid-phase method is described to create subclones, suitable for DNA sequencing, from lambda or cosmid libraries. The purified target DNA is sonicated and two linkers, with one oligonucleotide biotinylated in the 5'-end, are ligated to the ends of the fragments produced by sonication. After size separation, the fra...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(94)90038-8

    authors： Hultman T,Uhlén M

    更新日期：1994-06-30 00:00:00

  • Novel microbial epoxide hydrolases for biohydrolysis of glycidyl derivatives.

    abstract：:Microbial isolates from biofilters and petroleum-polluted bioremediation sites were screened for the presence of enantioselective epoxide hydrolases active towards tert-butyl glycidyl ether, benzyl glycidyl ether, and allyl glycidyl ether. Out of 270 isolated strains, which comprised bacteria, yeasts, and filamentous ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.06.011

    authors： Kotik M,Brichac J,Kyslík P

    更新日期：2005-12-06 00:00:00

  • Complete genome sequence of Thialkalivibrio versutus D301 isolated from Soda Lake in northern China, a typical strain with great ability to oxidize sulfide.

    abstract：:Thioalkalivibrio versutus D301 isolated from Soda Lake is a haloalkaliphilic and obligated chemolithoautotrophic Gram-negative bacterium. The strain has a good adaption to hyperhaline and highly alkaline environment and a powerful sulfur-oxidizing ability. Here, we present the complete genome sequence of T. versutus D...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.04.019

    authors： Mu T,Zhou J,Yang M,Xing J

    更新日期：2016-06-10 00:00:00

  • Comparison of mutant forms of the green fluorescent protein as expression markers in Chinese hamster ovary (CHO) and Saccharomyces cerevisiae cells.

    abstract：:Several green fluorescent protein (Gfp) mutants with increased cellular fluorescence compared to the wildtype protein have recently been generated. We have expressed and compared wildtype Gfp and mutants S65T, F100S/ M154T/V164A, F64L/S65T, and S65A/V68L/S72A under identical growth conditions in CHO and Saccharomyces ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(98)00040-6

    authors： Natarajan A,Subramanian S,Srienc F

    更新日期：1998-06-11 00:00:00

  • DNA vaccination of mice with a plasmid encoding Puumala hantavirus nucleocapsid protein mimics the B-cell response induced by virus infection.

    abstract：:Inoculation of naked DNA has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. To study the humoral immune response induced by DNA vaccination we cloned the entire nucleocapsid protein-encoding sequence of the Puumala hantavirus strain Vranica/Hällnäs into th...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00329-1

    authors： Koletzki D,Schirmbeck R,Lundkvist A,Meisel H,Krüger DH,Ulrich R

    更新日期：2001-11-17 00:00:00

  • Heterologous expression and functional characterization of a novel cellulose-disruptive protein LeEXP2 from Lycopersicum esculentum.

    abstract：:There has been little research reported on the synergism of plant expansins in cellulose hydrolysis because of the difficulty of heterologous expression. In this study, the expansin gene LeExp2 of Lycopersicum esculentum was expressed for the first time in Pichia pastoris to identify its function in vitro. The optimal...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.07.013

    authors： Liu X,Liu C,Ma Y,Hong J,Zhang M

    更新日期：2014-09-30 00:00:00

  • Synthesis, characterization of penicillin G capped silver nanoconjugates to combat β-lactamase resistance in infectious microorganism.

    abstract：:In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.12.002

    authors： Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NS

    更新日期：2013-02-20 00:00:00

  • Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation.

    abstract：:In Salvia miltiorrhiza hairy root cultures, the desired secondary metabolites diterpenoid tanshinones are normally produced at low yields and stored within the roots. To enhance tanshinone production and the secondary product recovery, we employed three means, elicitation with a yeast elicitor (YE), in situ adsorption...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.04.020

    authors： Yan Q,Hu Z,Tan RX,Wu J

    更新日期：2005-10-10 00:00:00

  • Enhanced production of β-glucosides by in-situ UDP-glucose regeneration.

    abstract：:Glycosyltransferase (GT)-mediated methodology is recognized as one of the most practical approaches for large-scale production of glycosides. However, GT enzymes require a sugar nucleotide as donor substrate that must be generated in situ for preparative applications by recycling of the nucleotide moiety, e.g. by sucr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.02.022

    authors： Huang FC,Hinkelmann J,Hermenau A,Schwab W

    更新日期：2016-04-20 00:00:00

  • Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley).

    abstract：:The establishment and optimization of in vitro primmorph formation from a Chinese sponge, Stylotella agminata (Ridley), collected from the South China Sea, were investigated. Our aims were to identify the key factors affecting primmorph formation in this species and to optimize the technique for developing an in vitro...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(02)00255-9

    authors： Zhang W,Zhang X,Cao X,Xu J,Zhao Q,Yu X,Jin M,Deng M

    更新日期：2003-01-23 00:00:00

  • Measurement of intracellular (compartmental) pH by 31P NMR in Aspergillus niger.

    abstract：:31P nuclear magnetic resonance (31P NMR) was used to monitor cytoplasmic and vacuolar pH values in the filamentous fungus Aspergillus niger. To obtain a homogeneous cell sample and to be able to perform long term in vivo NMR measurements A. niger mycelium was kept in a setup that allows perfusion of the cell plug with...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(99)00203-5

    authors： Hesse SJ,Ruijter GJ,Dijkema C,Visser J

    更新日期：2000-01-28 00:00:00

  • Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles.

    abstract：:Efficient dynamic interactions among cofactor, enzymes and substrate molecules are of primary importance for multi-step enzymatic reactions with in situ cofactor regeneration. Here we showed for the first time that the above dynamic interactions could be significantly intensified by exerting an external alternating ma...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.05.016

    authors： Zheng M,Su Z,Ji X,Ma G,Wang P,Zhang S

    更新日期：2013-10-20 00:00:00

  • Stability and stabilization of globular proteins in solution.

    abstract：:Proteins are multifunctional: their amino acid sequences simultaneously determine folding, function and turnover. Correspondingly, evolution selected for compromises between rigidity (stability) and flexibility (folding/function/degradation), to the result that generally the free energy of stabilization of globular pr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1656(00)00236-4

    authors： Jaenicke R

    更新日期：2000-05-26 00:00:00

  • Fusion tags and chaperone co-expression modulate both the solubility and the inclusion body features of the recombinant CLIPB14 serine protease.

    abstract：:Chaperone co-expression and the fusion to different tags were used to modify the aggregation pattern of the putative serine protease CLIPB14 precipitated in Escherichia coli inclusion bodies. A set of common tags used in expression vectors has been selected, as well as two bacterial strains over-expressing the chapero...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.04.028

    authors： Schrödel A,Volz J,de Marco A

    更新日期：2005-10-17 00:00:00

  • Genetic engineering of the Trichoderma reesei endoglucanase I (Cel7B) for enhanced partitioning in aqueous two-phase systems containing thermoseparating ethylene oxide--propylene oxide copolymers.

    abstract：:Endoglucanases (endo-1,4-beta-D-glucan-4-glucanohydrolase, EC 3.2.1.4) are industrially important enzymes. In this study endoglucanase I (EGI or Cel7B) of the filamentous fungi Trichoderma reesei has been genetically engineered to investigate the influence of tryptophan rich peptide extensions (tags) on partitioning i...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(01)00241-3

    authors： Collén A,Ward M,Tjerneld F,Stålbrand H

    更新日期：2001-05-04 00:00:00

  • Stoichiometric and kinetic characterisation of Nitrosomonas sp. in mixed culture by decoupling the growth and energy generation processes.

    abstract：:A novel method that relies on the decoupling of the energy production and biosynthesis processes was used to characterise the maintenance, cell lysis and growth processes of Nitrosomonas sp. A Nitrosomonas culture was enriched in a sequencing batch reactor (SBR) with ammonium as the sole energy source. Fluorescent in ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.04.017

    authors： Vadivelu VM,Keller J,Yuan Z

    更新日期：2006-11-10 00:00:00

  • Expression of active recombinant human alpha 1-antitrypsin in transgenic rabbits.

    abstract：:A DNA construct containing the human alpha 1-antitrypsin gene including 1.5 and 4 kb of 5' and 3' flanking sequences, was microinjected into the pronucleus of rabbit embryos. The recombinant human protein was (a) expressed in the blood circulation of F0 and F1 transgenic rabbits at an average concentration of 1 mg ml-...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(91)90247-s

    authors： Massoud M,Bischoff R,Dalemans W,Pointu H,Attal J,Schultz H,Clesse D,Stinnakre MG,Pavirani A,Houdebine LM

    更新日期：1991-05-01 00:00:00

  • Enoate reductases from non conventional yeasts: bioconversion, cloning, and functional expression in Saccharomyces cerevisiae.

    abstract：:Old yellow enzymes (OYEs, EC 1.6.99.1) are flavin-dependent oxidoreductases that catalyze the stereoselective trans-hydrogenation of the double bond, representing a promising alternative to metal-based catalysis. Bioconversion of ketoisophorone (KIP) by 28 non-conventional yeasts belonging to 16 different species was ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.08.033

    authors： Raimondi S,Romano D,Amaretti A,Molinari F,Rossi M

    更新日期：2011-12-20 00:00:00