Stoichiometric and kinetic characterisation of Nitrosomonas sp. in mixed culture by decoupling the growth and energy generation processes.


:A novel method that relies on the decoupling of the energy production and biosynthesis processes was used to characterise the maintenance, cell lysis and growth processes of Nitrosomonas sp. A Nitrosomonas culture was enriched in a sequencing batch reactor (SBR) with ammonium as the sole energy source. Fluorescent in situ hybridization (FISH) showed that Nitrosomonas bound to the NEU probe constituted 82% of the bacterial population, while no other known ammonium or nitrite oxidizing bacteria were detected. Batch tests were carried out under conditions that both ammonium and CO2 were in excess, and in the absence of one of these two substrates. The oxygen uptake rate and nitrite production rate were measured during these batch tests. The results obtained from these batch tests, along with the SBR performance data, allowed the determination of the maintenance coefficient and the in situ cell lysis rate, as well as the maximum specific growth rate of the Nitrosomonas culture. It is shown that, during normal growth, the Nitrosomonas culture spends approximately 65% of the energy generated for maintenance. The maintenance coefficient was determined to be 0.14-0.16 mgN mgCOD(biomass)(-1)h(-1), and was shown to be independent of the specific growth rate. The in situ lysis rate and the maximum specific growth rate of the Nitrosomonas culture were determined to be 0.26 and 1.0 day(-1) (0.043 h(-1)), respectively, under aerobic conditions at 30 degrees C and pH 7.


J Biotechnol


Journal of biotechnology


Vadivelu VM,Keller J,Yuan Z




Has Abstract


2006-11-10 00:00:00














  • Hydrolysis of softwood by Aspergillus mannanase: role of a carbohydrate-binding module.

    abstract::Endo beta-1,4-mannanases (beta-mannanases, EC, belonging to CAZy GH5 and GH26 families, catalyze the hydrolysis of structurally different mannans. In this study, the mannanase encoding gene of Aspergillus aculeatus VN was expressed in Aspergillus niger D15#26 using pAN 52-4 vector, under the control of PgpdA...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Pham TA,Berrin JG,Record E,To KA,Sigoillot JC

    更新日期:2010-08-02 00:00:00

  • Targeted delivery of calreticulin to ED-A fibronectin leads to tumor-growth retardation.

    abstract::We report the design and characterization of novel fusion proteins, consisting of the F8 antibody and of murine calreticulin (Calr). The F8 antibody recognizes the alternatively-spliced ED-A domain of fibronectin, an extracellular matrix component found in most tumor types, while calreticulin has previously been descr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ziffels B,Grötsch A,Al-Bayati L,Neri D

    更新日期:2019-01-20 00:00:00

  • Evaluation of kojic acid production in a repeated-batch PCS biofilm reactor.

    abstract::In this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by Aspergillus oryzae (A. oryzae), which was immobilized in plastic composite support (PCS) bioreactor. Nitrogen deficient medium was applied to increase the production of KA in PCS-immobilized bioreactor. The efficie...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Liu JM,Yu TC,Lin SP,Hsu RJ,Hsu KD,Cheng KC

    更新日期:2016-01-20 00:00:00

  • A novel approach for the construction of plant amiRNA expression vectors.

    abstract::Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

    更新日期:2011-01-10 00:00:00

  • Serum-free production of rVSV-ZEBOV in Vero cells: Microcarrier bioreactor versus scale-X™ hydro fixed-bed.

    abstract::Ebola virus disease outbreaks have repeatedly occurred on the African continent over the last decades, with more serious outbreaks in recent years. Being highly transmissible and associated to high fatality rates, it constitutes a serious threat to public health. Vaccination, however, may allow for efficient control o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kiesslich S,Vila-Chã Losa JP,Gélinas JF,Kamen AA

    更新日期:2020-02-20 00:00:00

  • KNIME for reproducible cross-domain analysis of life science data.

    abstract::Experiments in the life sciences often involve tools from a variety of domains such as mass spectrometry, next generation sequencing, or image processing. Passing the data between those tools often involves complex scripts for controlling data flow, data transformation, and statistical analysis. Such scripts are not o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Fillbrunn A,Dietz C,Pfeuffer J,Rahn R,Landrum GA,Berthold MR

    更新日期:2017-11-10 00:00:00

  • Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation.

    abstract::In Salvia miltiorrhiza hairy root cultures, the desired secondary metabolites diterpenoid tanshinones are normally produced at low yields and stored within the roots. To enhance tanshinone production and the secondary product recovery, we employed three means, elicitation with a yeast elicitor (YE), in situ adsorption...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yan Q,Hu Z,Tan RX,Wu J

    更新日期:2005-10-10 00:00:00

  • Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies.

    abstract::Traditional strategies for production of thermo-induced recombinant protein in Escherichia coli consist of a two-phase culture, with an initial growth stage at low temperature (commonly 30°C) followed by a production stage where temperature is increased stepwise (commonly up to 42°C). A disadvantage of such strategies...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Caspeta L,Lara AR,Pérez NO,Flores N,Bolívar F,Ramírez OT

    更新日期:2013-08-10 00:00:00

  • Improvement of resistance to maize dwarf mosaic virus mediated by transgenic RNA interference.

    abstract::To overcome the low efficiency of agronomic protection from maize dwarf mosaic disease, susceptible maize inbred line was transformed by Agrobacterium tumefaciens harboring hpRNA expression vectors containing inverted-repeat sequences of different lengths targeting coat protein gene (CP) of maize dwarf mosaic virus (M...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhang ZY,Yang L,Zhou SF,Wang HG,Li WC,Fu FL

    更新日期:2011-05-20 00:00:00

  • Transgenic rabbits as bioreactors for the production of human growth hormone.

    abstract::Gene farming is one of the most promising areas in modern biotechnology. To assay the potential usefulness of transgenic rabbits as bioreactors, one call embryos were microinjected with a chimeric gene comprising 5' sequences from mouse whey acidic protein gene (mWAP) linked to the human growth hormone (hGH) gene. Tra...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Limonta JM,Castro FO,Martínez R,Puentes P,Ramos B,Aguilar A,Lleonart RL,de la Fuente J

    更新日期:1995-05-15 00:00:00

  • Vitamin D fortification of foods and prospective health outcomes.

    abstract::Vitamin D is essential for bone health and has significant roles in non-skeletal health and organ function. Dermal synthesis through exposure to ultraviolet B light is the major natural source of vitamin D, while only a small portion of the necessary amount can be acquired by a diet without fortified foods. In recent ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Moulas AN,Vaiou M

    更新日期:2018-11-10 00:00:00

  • Characterization of the promoter and upstream activating sequence from the Pseudomonas alcaligenes lipase gene.

    abstract::Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Cox M,Gerritse G,Dankmeyer L,Quax WJ

    更新日期:2001-03-09 00:00:00

  • Recent developments in the structural organization and regulation of nitrogen fixation genes in Herbaspirillum seropedicae.

    abstract::Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

    更新日期:2001-10-04 00:00:00

  • A novel bispecific antibody targeting tumor necrosis factor α and ED-B fibronectin effectively inhibits the progression of established collagen-induce arthritis.

    abstract::Specific delivery of TNF-α antagonist to the inflamed site can increase its efficacy and reduce the side effects. In this study, we constructed a bispecific diabody (BsDb) that targets TNF-α and ED-B-containing fibronectin (B-FN), a fibronectin isoform specifically expressed in the pannus of the inflamed joint in rheu...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Liu M,Xie M,Jiang S,Liu G,Li L,Liu D,Yang X

    更新日期:2014-09-30 00:00:00

  • Multidrug resistance as a dominant molecular marker in transformation of wine yeast.

    abstract::Pure wine yeast cultures are increasingly used in winemaking to perform controlled fermentations and produce wine of reproducible quality. For the genetic manipulation of natural wine yeast strains dominant selective markers are obviously useful. Here we demonstrate the successful use of the mutated PDR3 gene as a dom...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kozovska Z,Maraz A,Magyar I,Subik J

    更新日期:2001-12-14 00:00:00

  • Production of ω-hydroxy octanoic acid with Escherichia coli.

    abstract::The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kirtz M,Klebensberger J,Otte KB,Richter SM,Hauer B

    更新日期:2016-07-20 00:00:00

  • Rapid degradation of ferulic acid via 4-vinylguaiacol and vanillin by a newly isolated strain of bacillus coagulans.

    abstract::A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was furthe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RC

    更新日期:2000-07-14 00:00:00

  • Production of 5-methyluridine by immobilized thermostable purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus JTS 859.

    abstract::5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degree...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hori N,Watanabe M,Sunagawa K,Uehara K,Mikami Y

    更新日期:1991-02-01 00:00:00

  • Biotransformation of phloretin by amylosucrase yields three novel dihydrochalcone glucosides.

    abstract::Glycosylation is one of the most important tailoring reactions for natural products. It typically exerts profound direct or indirect effects on their biological activity. The dihydrochalcone phloretin and its known sugar derivatives, particularly phlori(d)zin, have been shown to influence various cellular processes. W...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Overwin H,Wray V,Hofer B

    更新日期:2015-10-10 00:00:00

  • Methodological improvements of pyrosequencing technology.

    abstract::Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide poly...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gharizadeh B,Akhras M,Nourizad N,Ghaderi M,Yasuda K,Nyrén P,Pourmand N

    更新日期:2006-07-25 00:00:00

  • Optimization of lactic acid production using immobilized Lactobacillus Rhamnosus and carob pod waste from the Lebanese food industry.

    abstract::The valorization of a solid carob waste from the Lebanese industry was investigated by optimizing the production of lactic acid using immobilized Lactobacillus rhamnosus in alginate beads and response surface methodology. The results showed that pH and alginate concentration had a significant effect on the production ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Bahry H,Abdalla R,Pons A,Taha S,Vial C

    更新日期:2019-12-20 00:00:00

  • Enhanced production of β-glucosides by in-situ UDP-glucose regeneration.

    abstract::Glycosyltransferase (GT)-mediated methodology is recognized as one of the most practical approaches for large-scale production of glycosides. However, GT enzymes require a sugar nucleotide as donor substrate that must be generated in situ for preparative applications by recycling of the nucleotide moiety, e.g. by sucr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Huang FC,Hinkelmann J,Hermenau A,Schwab W

    更新日期:2016-04-20 00:00:00

  • Influence of transposition and insertion of additional binding domain on expression and characteristics of xylanase C of Clostridium thermocellum.

    abstract::Clostridium thermocellum encodes a xylanase gene (xynC) which is the major component of its cellulosome. XynC is a multidomain enzyme comprising of a substrate binding domain at the N-terminal followed by the catalytic domain and a dockerin domain. To study the influence of binding domain on activity, stability and ex...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Khan MI,Sajjad M,Ali I,Ahmad S,Akhtar MW

    更新日期:2010-10-01 00:00:00

  • Exploiting the metabolism of PYC expressing HEK293 cells in fed-batch cultures.

    abstract::The expression of recombinant yeast pyruvate carboxylase (PYC) in animal cell lines was shown in previous studies to reduce significantly the formation of waste metabolites, although it has translated into mixed results in terms of improved cellular growth and productivity. In this work, we demonstrate that the unique...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Vallée C,Durocher Y,Henry O

    更新日期:2014-01-01 00:00:00

  • An automated spectrophotometric flow-injection procedure for the determination of cellulase activity in bioreactor preparations.

    abstract::A spectrophotometric procedure for the determination of cellulase activity in precipitated bioreactor preparations and culture filtrates is described. It is based on the determination of reducing sugar produced by the action of the enzyme on carboxymethylcellulose. The reducing sugar is derivatized with p-aminobenzoyl...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Worsfold PJ,Whiteside IR,Pfeiffer HF,Waldhoff H

    更新日期:1990-04-01 00:00:00

  • The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla).

    abstract::European eel (Anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3',4,4'-tetrachlorobiyphenyl (PCB77) to examine and characterize the inductive effect of coplanar PCBs on CYP1A1 gene expression in liver and gills by using a semi-quantitative RT-PCR analysis. The influence of PCB77 inje...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Marohn L,Rehbein H,Kündiger R,Hanel R

    更新日期:2008-09-10 00:00:00

  • Enoate reductases from non conventional yeasts: bioconversion, cloning, and functional expression in Saccharomyces cerevisiae.

    abstract::Old yellow enzymes (OYEs, EC are flavin-dependent oxidoreductases that catalyze the stereoselective trans-hydrogenation of the double bond, representing a promising alternative to metal-based catalysis. Bioconversion of ketoisophorone (KIP) by 28 non-conventional yeasts belonging to 16 different species was ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Raimondi S,Romano D,Amaretti A,Molinari F,Rossi M

    更新日期:2011-12-20 00:00:00

  • New N-acyl-D-glucosamine 2-epimerases from cyanobacteria with high activity in the absence of ATP and low inhibition by pyruvate.

    abstract::N-Acetylneuraminic acid, an important component of glycoconjugates with various biological functions, can be produced from N-acetyl-D-glucosamine (GlcNAc) and pyruvate using a one-pot, two-enzyme system consisting of N-acyl-D-glucosamine 2-epimerase (AGE) and N-acetylneuraminate lyase (NAL). In this system, the epimer...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Klermund L,Groher A,Castiglione K

    更新日期:2013-11-01 00:00:00

  • Mechanistic understanding of the cysteine capping modifications of antibodies enables selective chemical engineering in live mammalian cells.

    abstract::Protein modifications by intricate cellular machineries often redesign the structure and function of existing proteins to impact biological networks. Disulfide bond formation between cysteine (Cys) pairs is one of the most common modifications found in extracellularly-destined proteins, key to maintaining protein stru...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhong X,He T,Prashad AS,Wang W,Cohen J,Ferguson D,Tam AS,Sousa E,Lin L,Tchistiakova L,Gatto S,D'Antona A,Luan YT,Ma W,Zollner R,Zhou J,Arve B,Somers W,Kriz R

    更新日期:2017-04-20 00:00:00

  • Production of targeted poly(3-hydroxyalkanoates) copolymers by glycogen accumulating organisms using acetate as sole carbon source.

    abstract::One of the main limitations in bacterial polyhydroxyalkanoate (PHA) production with mixed cultures is the fact that primarily polyhydroxybutyrate (PHB) homopolymers are generated from acetate as the main carbon source, which is brittle and quite fragile. The incorporation of different 3-hydroxyalkanoate (HA) component...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Dai Y,Yuan Z,Jack K,Keller J

    更新日期:2007-05-01 00:00:00