Abstract：
:Recombinant bacteriophage Qbeta coat protein (CP), which has been proposed as a promising carrier of foreign epitopes via their incorporation either by gene engineering techniques or by chemical coupling, efficiently self-assembles into virus-like particles (VLPs) when expressed in Escherichia coli. Here, we demonstrate expression and self-assembly of Qbeta CP in yeast Saccharomyces cerevisiae and Pichia pastoris. Production reached 3-4 mg/1g of wet cells for S. cerevisiae and 4-6 mg for P. pastoris, which was about 15-20% and 20-30% of the E. coli expression level, respectively. Qbeta VLPs were easily purified by size-exclusion chromatography in both cases and contained nucleic acid, shown by native agarose gel electrophoresis. The obtained particles were highly immunogenic in mice and the resulting sera recognized both E. coli- and yeast-derived Qbeta VLPs equally well.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Freivalds J,Dislers A,Ose V,Skrastina D,Cielens I,Pumpens P,Sasnauskas K,Kazaks Adoi
10.1016/j.jbiotec.2005.11.013subject
Has Abstractpub_date
2006-05-29 00:00:00pages
297-303issue
3eissn
0168-1656issn
1873-4863pii
S0168-1656(05)00749-2journal_volume
123pub_type
杂志文章abstract：:ACC deaminase producing Plant growth promoting rhizobacteria (PGPR) offers a great promise for ameliorating the negative impacts of salinity stress manifested on plants. In this context, 28 rhizospheric bacteria associated with ACC deaminase potential (198-1069 nmol α-ketobutyrate mg protein-1 h-1) were isolated from ...
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pub_type： 杂志文章
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abstract：:Genome shuffling is an efficient way to pool advantageous traits during positive selections of industrial microorganisms. In this study, for the first time, the effectiveness of genome shuffling to improve yielding of heterologous proteins was investigated in Kluyveromyces marxianus (KM), a promising yeast host. After...
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2014-11-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2019-07-20 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2015-11-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.03.016
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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