Abstract:
:The partitioning of proteins and other biomaterials between two aqueous phases containing polyethyleneglycol and dextran is a strong function of the molecular weight of the two polymers. Although both polymers are polydispersed (especially Dx) most theoretical treatments refer only to the average molecular weight (number or mass) and assume that the molecular weight distribution of each polymer is the same in both phases. In this work the molecular weight distribution of each polymer is the same in both phases. In this work the molecular weight distributions of four stock solutions of PEG (4000, 6000, 10,000 and 20,000) and four stock solutions of Dx (10,000, 40,000, 110,000 and 500,000) were measured using High Performance Gel Chromatography. The measurements were repeated on the phases formed by the polymer solutions after they were mixed and allowed to equilibrate. The molecular weight distribution of the Dx differed in the top and bottom phase; both differed from that of the stock solution. Although we believe that the molecular weight distribution for PEG also differs in the top and bottom phases, we were unable to determine this within the resolution of our instruments.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Forciniti D,Hall CK,Kula MRdoi
10.1016/0168-1656(91)90224-jsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
151-61issue
2eissn
0168-1656issn
1873-4863pii
0168-1656(91)90224-Jjournal_volume
20pub_type
杂志文章abstract::Cannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella p...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2018.07.031
更新日期:2018-10-20 00:00:00
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pub_type: 杂志文章
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更新日期:2005-06-29 00:00:00
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journal_title:Journal of biotechnology
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更新日期:2001-04-13 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(01)00277-2
更新日期:2001-07-12 00:00:00
abstract::A murine B-lymphocyte hybridoma (CC9C10) was adapted for growth in a serum-free medium. Supplementation of the medium with cis-unsaturated fatty acids (10-50 microM) improved the cell yield in the order oleic/linoleic > linoleic > oleic. Initial supplementation with the fatty acids also caused a significant increase (...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(95)00017-k
更新日期:1995-04-15 00:00:00
abstract::Recombinant Volvariella volvacea endoglucanase 1 (EG1) and its catalytic module (EG1-CM) were obtained by expression in Pichia pastoris, purified by two-step chromatography, and the catalytic activities and binding capacities were compared. EG1 and EG1-CM exhibited very similar specific activities towards the soluble ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.05.016
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2015-01-20 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.06.010
更新日期:2007-10-31 00:00:00
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journal_title:Journal of biotechnology
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更新日期:2002-01-18 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
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更新日期:1996-01-26 00:00:00
abstract::(+)-Nootkatone is a natural ingredient that occurs in grapefruit and certain other plants and is responsible for the characteristic smell of grapefruit. Due to its versatile applications in the flavor and fragrance industry as well as its application in some medical uses it recruits the interests of academic research ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2019-08-10 00:00:00
abstract::Clostridium cellulovorans produces large extracellular enzyme complex, called cellulosomes. The diversity of the cellulosomal enzymes, which are secreted by C. cellulovorans that has been cultured on different carbon sources, such as Avicel, xylan, AXP (Avicel-xylan-pectin, 3:1:1) and cellobiose, was explored by two-d...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2009.11.020
更新日期:2010-02-01 00:00:00
abstract::Terminal sialic acid on oligosaccharides of glycoproteins shows several biological functions of the glycoproteins. The yeast Pichia pastoris normally does not contain sialic acid on the oligosaccharides of glycoproteins. A sialyltransferase (ST) gene was transfected into P. pastoris to assess the possibility of using ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(00)00268-6
更新日期:2000-07-28 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2009.02.009
更新日期:2009-04-20 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2013.07.006
更新日期:2013-09-10 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2005.08.031
更新日期:2006-03-09 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:1998-02-05 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2018-12-10 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2003-05-08 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:1996-10-18 00:00:00
abstract::Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2009.03.004
更新日期:2009-05-20 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2009-01-15 00:00:00
abstract::The configuration and performance of the Biorack facility during the Second International Microgravity Laboratory mission (IML-2; 8-23 July 1995) is described in detail. During this Spacelab mission, Biorack flew with two incubators (22 degrees C and 37 degrees C), glovebox, cooler (5 degrees C) and four passive ther...
journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:1996-06-27 00:00:00
abstract::There has been little research reported on the synergism of plant expansins in cellulose hydrolysis because of the difficulty of heterologous expression. In this study, the expansin gene LeExp2 of Lycopersicum esculentum was expressed for the first time in Pichia pastoris to identify its function in vitro. The optimal...
journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2014-09-30 00:00:00