Abstract：
:Terminal sialic acid on oligosaccharides of glycoproteins shows several biological functions of the glycoproteins. The yeast Pichia pastoris normally does not contain sialic acid on the oligosaccharides of glycoproteins. A sialyltransferase (ST) gene was transfected into P. pastoris to assess the possibility of using yeast cells as a host to produce sialoglycoproteins. The expression vectors pPIC3.5 and pPIC9 were used as carriers. The recombinant P. pastoris harbouring ST-pPIC3.5 and ST-pPIC9 had sialyltransferase activity of 1.1 and 10.2 mU l(-1) respectively. The ability of the recombinant ST-pPIC3.5 and ST-pPIC9 to transfer the fluoresceinyl-NeuAc into the cell glycoproteins was 36.9 and 20.9 pmol mg -1 protein respectively.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Chotigeat W,Chayanunnukul W,Phongdara Adoi
10.1016/s0168-1656(00)00268-6subject
Has Abstractpub_date
2000-07-28 00:00:00pages
55-61issue
1eissn
0168-1656issn
1873-4863pii
S0168-1656(00)00268-6journal_volume
81pub_type
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