Wafer-scale nanowell array patterning based electrochemical impedimetric immunosensor.

Abstract:

:We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In this research, we developed an impedimetric immunosensor based on wafer-scale NWA for electrochemical detection of stress-induced-phosphoprotein-1 (STIP-1). In order to develop NWA sensor through the cost-effective combination of high-throughput nanopattern, the NWA electrode was fabricated on Si wafer by krypton-fluoride (KrF) stepper semiconductor process. Finally, 12,500,000 ea nanowell with a 500 nm diameter was fabricated on 4 mm × 2 mm substrate. Next, by using these electrodes, we measured impedance to quantify antigen binding to the immunoaffinity layer. The limit of detection (LOD) of the NWA was improved about 100-fold compared to milli-sized electrodes (4 mm × 2 mm) without an NWA. These results suggest that wafer-scale NWA immunosensor will be useful for biosensing applications because their interface response is appropriate for detecting molecular binding events.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Lee J,Cho S,Lee J,Ryu H,Park J,Lim S,Oh B,Lee C,Huang W,Busnaina A,Lee H

doi

10.1016/j.jbiotec.2013.08.029

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

584-8

issue

4

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(13)00380-5

journal_volume

168

pub_type

杂志文章
  • Genome improvement of the acarbose producer Actinoplanes sp. SE50/110 and annotation refinement based on RNA-seq analysis.

    abstract::Actinoplanes sp. SE50/110 is the natural producer of acarbose, which is used in the treatment of diabetes mellitus type II. However, until now the transcriptional organization and regulation of the acarbose biosynthesis are only understood rudimentarily. The genome sequence of Actinoplanes sp. SE50/110 was known befor...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.04.013

    authors: Wolf T,Schneiker-Bekel S,Neshat A,Ortseifen V,Wibberg D,Zemke T,Pühler A,Kalinowski J

    更新日期:2017-06-10 00:00:00

  • Laser reflectance measurement for the online monitoring of Chlorella sorokiniana biomass concentration.

    abstract::Fast and reliable methods to determine biomass concentration are necessary to facilitate the large scale production of microalgae. A method for the rapid estimation of Chlorella sorokiniana biomass concentration was developed. The method translates the suspension particle size spectrum gathered though laser reflectanc...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.12.020

    authors: López Expósito P,Blanco Suárez A,Negro Álvarez C

    更新日期:2017-02-10 00:00:00

  • Lipid production by Lipomyces starkeyi cells in glucose solution without auxiliary nutrients.

    abstract::Two-stage fermentation process was used for lipid production by Lipomyces starkeyi AS 2.1560 in glucose solution without auxiliary nutrients. In the first stage, cells were cultivated in a nutrient-rich medium for propagation. In the second stage, cells were resuspended in glucose solution to achieve high cellular lip...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.02.010

    authors: Lin J,Shen H,Tan H,Zhao X,Wu S,Hu C,Zhao ZK

    更新日期:2011-04-10 00:00:00

  • Oligonucleotide array for detection of common severe determinants of alpha thalassemia.

    abstract::A simple and an efficient oligonucleotide array was developed to identify common severe determinants of alpha (alpha) thalassemia. A total of 14 probes were designed to detect the most frequently three deletions (-alpha(3.7), -alpha(4.2), -(SEA)) and two non-deletions (alpha(Quong Sze), alpha(Constant Spring)). PCR pr...

    journal_title:Journal of biotechnology

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.jbiotec.2004.07.016

    authors: Ye BC,Zhang Z,Lei Z

    更新日期:2005-01-12 00:00:00

  • Development of grapevine somatic embryogenesis using an air-lift bioreactor as an efficient tool in the generation of transgenic plants.

    abstract::The grapevine genetic transformation programs have relayed on the use of solid media-based somatic embryogenesis. To reach a high throughput of candidate gene evaluation in 'Thompson Seedless', a semi-automatic system allowing viable transformation of explants was designed. An intermediate procedure using liquid media...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2008.09.009

    authors: Tapia E,Sequeida A,Castro A,Montes C,Zamora P,López R,Acevedo F,Prieto H

    更新日期:2009-01-01 00:00:00

  • Construction of an efficient xylose-fermenting diploid Saccharomyces cerevisiae strain through mating of two engineered haploid strains capable of xylose assimilation.

    abstract::Saccharomyces cerevisiae can be engineered for xylose fermentation through introduction of wild type or mutant genes (XYL1/XYL1 (R276H), XYL2, and XYL3) coding for xylose metabolic enzymes from Scheffersomyces stipitis. The resulting engineered strains, however, often yielded undesirable phenotypes such as slow xylose...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2012.12.012

    authors: Kim SR,Lee KS,Kong II,Lesmana A,Lee WH,Seo JH,Kweon DH,Jin YS

    更新日期:2013-03-10 00:00:00

  • How do lipases and esterases work: the electrostatic contribution.

    abstract::This work explores the role of one of the factors explaining lipase/esterase activity: the contribution of electrostatic interactions to lipase/esterase activity. The electrostatic potential distribution on the molecular surface of an enzyme as a function of pH determines, to a large extent, the enzyme's pH activity p...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(00)00360-6

    authors: Neves Petersen MT,Fojan P,Petersen SB

    更新日期:2001-02-13 00:00:00

  • Bacterial ghosts: non-living candidate vaccines.

    abstract::Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/0168-1656(95)00123-9

    authors: Szostak MP,Hensel A,Eko FO,Klein R,Auer T,Mader H,Haslberger A,Bunka S,Wanner G,Lubitz W

    更新日期:1996-01-26 00:00:00

  • Kinetics of microbial growth and biodegradation of methanol and toluene in biofilters and an analysis of the energetic indicators.

    abstract::The kinetics of microbial growth and the biodegradation of methanol and toluene in (a) biofilters (BFs), and (b) biotrickling filters (BTFs), packed with inert materials, has been studied and analyzed. The specific growth rate, mu, for the treatment of methanol was 0.037h(-1) for a wide range of operating conditions. ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2008.08.001

    authors: Avalos Ramirez A,Bénard S,Giroir-Fendler A,Jones JP,Heitz M

    更新日期:2008-11-25 00:00:00

  • In situ-immobilization of two model cyanobacterial strains in ceramic structures: A new biohybrid material for photobioreactor applications.

    abstract::Two cyanobacterial strains, Synechocystis sp. PCC 6803 and Nostoc punctiforme ATCC 29133 were immobilized within magnesium phosphate based cements, showing a viability and activity for at least 4 weeks. These biohybrids are considered as an alternative photobioreactor material for bioremediation or an improved yield o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.02.024

    authors: Vorndran E,Lindberg P

    更新日期:2016-04-10 00:00:00

  • Isolation, taxonomic analysis, and phenotypic characterization of bacterial endophytes present in alfalfa (Medicago sativa) seeds.

    abstract::A growing body of evidence has reinforced the central role of microbiomes in the life of sound multicellular eukaryotes, thus more properly described as true holobionts. Though soil was considered a main source of plant microbiomes, seeds have been shown to be endophytically colonized by microorganisms thus representi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.12.020

    authors: López JL,Alvarez F,Príncipe A,Salas ME,Lozano MJ,Draghi WO,Jofré E,Lagares A

    更新日期:2018-02-10 00:00:00

  • Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation.

    abstract::In Salvia miltiorrhiza hairy root cultures, the desired secondary metabolites diterpenoid tanshinones are normally produced at low yields and stored within the roots. To enhance tanshinone production and the secondary product recovery, we employed three means, elicitation with a yeast elicitor (YE), in situ adsorption...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.04.020

    authors: Yan Q,Hu Z,Tan RX,Wu J

    更新日期:2005-10-10 00:00:00

  • Identification of StiR, the first regulator of secondary metabolite formation in the myxobacterium Cystobacter fuscus Cb f17.1.

    abstract::Myxobacteria are well established as proficient producers of natural products with numerous biological activities. Although some knowledge has been gained regarding the biosynthesis of secondary metabolites in this class of bacteria, almost nothing is known about the underlying regulatory mechanisms. In order to ident...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.08.014

    authors: Rachid S,Sasse F,Beyer S,Müller R

    更新日期:2006-02-24 00:00:00

  • Transcript stabilization by mRNA sequences from hrpA of Pseudomonas syringae.

    abstract::Production of heterologous proteins in bacteria is one of the main applications of biotechnology. Although several high-efficiency expression systems have been developed, different steps in protein production may become rate-limiting depending on the production system and the protein being produced. One bottle neck ca...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2006.10.010

    authors: Hienonen E,Romantschuk M,Fenel F,Taira S

    更新日期:2007-02-01 00:00:00

  • Methods for reducing the ammonia in hybridoma cell cultures.

    abstract::The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(94)00142-y

    authors: Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

    更新日期:1995-02-21 00:00:00

  • Net P-removal deterioration in enriched PAO sludge subjected to permanent aerobic conditions.

    abstract::Recently, some research in the field of enhanced biological phosphorus removal (EBPR) has been focused on studying systems where the electron donor (substrate) and the electron acceptor (nitrate or oxygen) are present simultaneously. This can occur, for example, in a full scale wastewater treatment plant during heavy ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.10.018

    authors: Pijuan M,Guisasola A,Baeza JA,Carrera J,Casas C,Lafuente J

    更新日期:2006-05-03 00:00:00

  • Oil sands tailings ponds harbour a small core prokaryotic microbiome and diverse accessory communities.

    abstract::Oil sands tailings ponds store the waste slurry generated by extracting bitumen from surface-mined oil (tar) sands ores. The ponds support diverse microbial communities involved in element cycling, greenhouse gas production, and hydrocarbon biodegradation that influence pond management and their environmental footprin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.06.030

    authors: Wilson SL,Li C,Ramos-Padrón E,Nesbø C,Soh J,Sensen CW,Voordouw G,Foght J,Gieg LM

    更新日期:2016-10-10 00:00:00

  • Synthesis and antibacterial activity of solanum torvum mediated silver nanoparticle against Xxanthomonas axonopodis pv.punicae and Ralstonia solanacearum.

    abstract::To meet the food demand of growing population, agricultural productivity needs to be increased by employing safe strategies without harming ecosystem. Silver nanoparticle (AgNP) using a green approach has become a promising substitute to the synthetic pesticides to overcome pest menace. In this study, AgNPs were synth...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2019.12.009

    authors: Vanti GL,Kurjogi M,Basavesha KN,Teradal NL,Masaphy S,Nargund VB

    更新日期:2020-02-10 00:00:00

  • Evaluation of various serum and animal protein free media for the production of a veterinary rabies vaccine in BHK-21 cells.

    abstract::We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch or a gradual adaptation, we have achieved BHK-21 cells growth in the following media: HyQ PF CHO, HyQ PF CHO MPS, Ren...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(02)00009-3

    authors: Kallel H,Jouini A,Majoul S,Rourou S

    更新日期:2002-05-23 00:00:00

  • Efficient function and characterization of GH10 xylanase (Xyl10g) from Gloeophyllum trabeum in lignocellulose degradation.

    abstract::The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.12.013

    authors: Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

    更新日期:2014-02-20 00:00:00

  • Solid-phase cloning to create sublibraries suitable for DNA sequencing.

    abstract::A solid-phase method is described to create subclones, suitable for DNA sequencing, from lambda or cosmid libraries. The purified target DNA is sonicated and two linkers, with one oligonucleotide biotinylated in the 5'-end, are ligated to the ends of the fragments produced by sonication. After size separation, the fra...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(94)90038-8

    authors: Hultman T,Uhlén M

    更新日期:1994-06-30 00:00:00

  • Reprint of Design of synthetic microbial communities for biotechnological production processes.

    abstract::In their natural habitats microorganisms live in multi-species communities, in which the community members exhibit complex metabolic interactions. In contrast, biotechnological production processes catalyzed by microorganisms are usually carried out with single strains in pure cultures. A number of production processe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jbiotec.2014.11.005

    authors: Jagmann N,Philipp B

    更新日期:2014-12-20 00:00:00

  • Feeding frequency affects cultured rat pituitary cells in low gravity.

    abstract::In this report, we describe the results of a rat pituitary cell culture experiment done on STS-65 in which the effect of cell feeding on the release of the six anterior pituitary hormones was studied. We found complex microgravity-related interactions between the frequency of cell feeding and the quantity and quality ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(96)01373-9

    authors: Hymer WC,Grindeland RE,Salada T,Cenci R,Krishnan K,Mukai C,Nagaoka S

    更新日期:1996-06-27 00:00:00

  • Efficient MILP formulations for the optimal synthesis of chromatographic protein purification processes.

    abstract::The use of recombinant proteins has increased greatly in recent years, as well as the techniques used for their purification. The selection of an efficient process to purify proteins is a major bottleneck found when trying to scale up results obtained in the laboratory to a large-scale industrial process. One of the m...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2004.02.009

    authors: Vásquez-Alvarez E,Pinto JM

    更新日期:2004-06-10 00:00:00

  • Hydrophobicity engineering to facilitate surface display of heterologous gene products on Staphylococcus xylosus.

    abstract::Protein engineering has been employed to investigate the effect of specific amino acid changes on the targeting of heterologous proteins to the outer cell surface of the Gram-positive bacterium Staphylococcus xylosus. Three different variants, corresponding to a 101 amino acid region of the major glycoprotein (G prote...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(95)00081-z

    authors: Nguyen TN,Gourdon MH,Hansson M,Robert A,Samuelson P,Libon C,Andréoni C,Nygren PA,Binz H,Uhlén M

    更新日期:1995-10-16 00:00:00

  • Differential induction of autophagy in caspase-3/7 down-regulating and Bcl-2 overexpressing recombinant CHO cells subjected to sodium butyrate treatment.

    abstract::Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2012.05.011

    authors: Lee JS,Kim YJ,Kim CL,Lee GM

    更新日期:2012-09-15 00:00:00

  • Heterologous production of different styrene oxide isomerases for the highly efficient synthesis of phenylacetaldehyde.

    abstract::The styrene oxide isomerase (SOI, StyC) represents a key enzyme of the styrene-degrading pathway and has been discussed as promising biocatalyst during recent studies. The enzyme enables the synthesis of pure phenylacetaldehyde from styrene oxide. In this study the native as well as the corresponding codon-optimized g...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.04.038

    authors: Oelschlägel M,Richter L,Stuhr A,Hofmann S,Schlömann M

    更新日期:2017-06-20 00:00:00

  • Complete genome sequence of Thialkalivibrio versutus D301 isolated from Soda Lake in northern China, a typical strain with great ability to oxidize sulfide.

    abstract::Thioalkalivibrio versutus D301 isolated from Soda Lake is a haloalkaliphilic and obligated chemolithoautotrophic Gram-negative bacterium. The strain has a good adaption to hyperhaline and highly alkaline environment and a powerful sulfur-oxidizing ability. Here, we present the complete genome sequence of T. versutus D...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.04.019

    authors: Mu T,Zhou J,Yang M,Xing J

    更新日期:2016-06-10 00:00:00

  • Rifampicin-resistance, rpoB polymorphism and RNA polymerase genetic engineering.

    abstract::Following its introduction in 1967, rifampicin has become a mainstay of therapy in the treatment of tuberculosis, leprosy and many other widespread diseases. Its potent antibacterial activity is due to specific inhibition of bacterial RNA polymerase. However, resistance to rifampicin was reported shortly after its int...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jbiotec.2014.11.024

    authors: Alifano P,Palumbo C,Pasanisi D,Talà A

    更新日期:2015-05-20 00:00:00

  • Rapid cultivation of stable aerobic phenol-degrading granules using acetate-fed granules as microbial seed.

    abstract::The aim of this study is to evaluate the utility of using aerobic acetate-fed microbial granules as a starting seed to rapidly develop stable aerobic phenol-degrading granules. Aerobic granules were first cultivated in four sequencing batch reactors with acetate as sole carbon source at a loading rate of 3.8 kg m(-3) ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2004.09.008

    authors: Tay ST,Moy BY,Jiang HL,Tay JH

    更新日期:2005-02-23 00:00:00