Abstract：
:New methods for an extended physiological characterization of yeast at a microtiter plate scale were applied to 27 deletion mutants of Saccharomyces cerevisiae cultivated on glucose and galactose as sole carbon sources. In this way, specific growth rates, specific rates of glucose consumption and ethanol production were determined. Flux distribution, particularly concerning branching into the pentose phosphate pathway was determined using a new (13)C-labelling method using MALDI-ToF-mass spectrometry. On glucose, the growth was predominantly fermentative whereas on galactose respiration was more active with correspondingly lower ethanol production. Some deletion strains showed unexpected behavior providing very informative data about the function of the corresponding gene. Deletion of malic enzyme gene, MAE1, did not show any significant phenotype when grown on glucose but a drastically increased branching from glucose 6-phosphate into the pentose phosphate pathway when grown on galactose. This allows the conclusion that MAE1 is important for the supply of NADPH during aerobic growth on galactose.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Velagapudi VR,Wittmann C,Schneider K,Heinzle Edoi
10.1016/j.jbiotec.2007.08.043subject
Has Abstractpub_date
2007-12-01 00:00:00pages
395-404issue
4eissn
0168-1656issn
1873-4863pii
S0168-1656(07)01537-4journal_volume
132pub_type
杂志文章abstract：:The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian ...
journal_title：Journal of biotechnology
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pub_type： 杂志文章,评审
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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journal_title：Journal of biotechnology
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2005-08-22 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.06.028
更新日期：2005-11-21 00:00:00
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journal_title：Journal of biotechnology
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