Diffusion in Pseudomonas aeruginosa biofilms: a pulsed field gradient NMR study.

abstract：:In n-hexadecane fermentation by Pseudomonas aeruginosa, the production of rhamnolipids (biosurfactants) caused by nitrogen source limitation was observed during the stationary phase. The rhamnolipids caused severe foaming in the process, particularly at higher pH (ca. > 6.8). To reduce the foaming, several runs were m...

journal_title：Journal of biotechnology

authors： Wu J,Ju LK

更新日期：1997-01-03 00:00:00

abstract：:Sulfite dissolving pulp from Eucalyptus grandis contained approximately 3.8% O-acetyl-4-O-methylglucuronoxylan with a molar ratio of xylose:4-O-methylglucuronic acid:acetyl group close to 13.6:1:6.2. The effects produced by purified endo-xylanases from two different glycosyl hydrolase families (family 10 and 11) as we...

journal_title：Journal of biotechnology

authors： Christov L,Biely P,Kalogeris E,Christakopoulos P,Prior BA,Bhat MK

更新日期：2000-10-13 00:00:00

abstract：:The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplan...

journal_title：Journal of biotechnology

authors： Chen Q,Zhang S,Xie Y

更新日期：2018-09-10 00:00:00

abstract：:Pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene, is associated with tumor formation, proliferation and invasiveness. F-PBF(beta-TrCP), a fusion protein, was produced by replacing the WD40-repeat of F-box protein beta-TrCP with the PTTG1-binding factor (PBF) for targeted degradation of PTTG1. To evaluate ...

journal_title：Journal of biotechnology

authors： Mo Z,Zu X,Xie Z,Li W,Ning H,Jiang Y,Xu W

更新日期：2009-01-01 00:00:00

abstract：:Sodium butyrate was used to enhance expression levels and thereby facilitate the generation of analytical quantities of nine different tissue plasminogen activator (tPA) analogues expressed under the control of the cytomegalovirus immediate early (CMV IE) promoter by the Chinese hamster ovary (CHO) mammalian expressio...

journal_title：Journal of biotechnology

authors： Palermo DP,DeGraaf ME,Marotti KR,Rehberg E,Post LE

更新日期：1991-06-01 00:00:00

abstract：:A model for transport of ammonia and ammonium ions across cell membranes is presented. The model suggests that ammonium ions compete with potassium ions for inward transport, over the cytoplasmic membrane, via potassium transport proteins like the Na+/K(+)-ATPase and the Na+K+2Cl(-)-cotransporter. It also explains the...

journal_title：Journal of biotechnology

authors： Martinelle K,Häggström L

更新日期：1993-09-01 00:00:00

abstract：:Saccharomyces cerevisiae can be engineered for xylose fermentation through introduction of wild type or mutant genes (XYL1/XYL1 (R276H), XYL2, and XYL3) coding for xylose metabolic enzymes from Scheffersomyces stipitis. The resulting engineered strains, however, often yielded undesirable phenotypes such as slow xylose...

journal_title：Journal of biotechnology

authors： Kim SR,Lee KS,Kong II,Lesmana A,Lee WH,Seo JH,Kweon DH,Jin YS

更新日期：2013-03-10 00:00:00

abstract：:A system has been developed for the release of heterologous proteins from Saccharomyces cerevisiae, based on the use of thermosensitive osmotic-remedial mutants, deficient in cell integrity, that lyse at the non-permissive temperature, thus releasing the bulk of intracellular proteins and leaving behind cell ghosts an...

journal_title：Journal of biotechnology

authors： Alvarez P,Sampedro M,Molina M,Nombela C

更新日期：1994-11-30 00:00:00

abstract：:We developed a detailed mathematical model describing the coupling between the molecular weight distribution dynamics of poly(3-hydroxybutyrate-co-3hydroxyvalerate) (PHBV) copolymer chains with those of hydroxybutyrate (HB) and hydroxyvalerate (HV) monomer formation. Sensitivity analysis of the model revealed that bot...

journal_title：Journal of biotechnology

authors： Iadevaia S,Mantzaris NV

更新日期：2006-03-09 00:00:00

abstract：:One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid DNA was prepared by a method that combines alkaline lysis of E. ...

journal_title：Journal of biotechnology

authors： Wright JL,Jordan M,Wurm FM

更新日期：2003-05-08 00:00:00

abstract：:The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate-graft-poly(ethylene glycol)/α-cyclodextrins (Alg-g-PEG/α-CD) spheres as enzyme immobilization carriers. The morphology and size o...

journal_title：Journal of biotechnology

authors： Hu C,Wang N,Zhang W,Zhang S,Meng Y,Yu X

更新日期：2015-01-20 00:00:00

abstract：:It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

journal_title：Journal of biotechnology

authors： Hatzis C,Porro D

更新日期：2006-07-13 00:00:00

abstract：:In this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by Aspergillus oryzae (A. oryzae), which was immobilized in plastic composite support (PCS) bioreactor. Nitrogen deficient medium was applied to increase the production of KA in PCS-immobilized bioreactor. The efficie...

journal_title：Journal of biotechnology

authors： Liu JM,Yu TC,Lin SP,Hsu RJ,Hsu KD,Cheng KC

更新日期：2016-01-20 00:00:00

abstract：:The mTOR pathway is a conserved master regulator of translational activity that influences the fate of industrially relevant CHO cell cultures, yet its molecular mechanisms remain unclear. Interestingly, rapamycin specific inhibition of the mTOR pathway in CHO cells was found to down-regulate the small nucleolar RNA U...

journal_title：Journal of biotechnology

authors： Courtes FC,Gu C,Wong NS,Dedon PC,Yap MG,Lee DY

更新日期：2014-03-20 00:00:00

abstract：:Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning ...

journal_title：Journal of biotechnology

authors： Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

更新日期：2011-01-10 00:00:00

abstract：:The partitioning of proteins and other biomaterials between two aqueous phases containing polyethyleneglycol and dextran is a strong function of the molecular weight of the two polymers. Although both polymers are polydispersed (especially Dx) most theoretical treatments refer only to the average molecular weight (num...

journal_title：Journal of biotechnology

authors： Forciniti D,Hall CK,Kula MR

更新日期：1991-09-01 00:00:00

abstract：:The poly(beta-hydroxybutyrate) (PHB) biosynthetic genes of Ralstonia eutropha that are organized in a single operon (phaCAB) have been cloned in Escherichia coli, where the expression of the genes in the wild-type pha operon from plasmid pTZ18U-PHB leads to the formation of 50-80% PHB/celldry mass when the cells are g...

journal_title：Journal of biotechnology

authors： Resch S,Gruber K,Wanner G,Slater S,Dennis D,Lubitz W

更新日期：1998-10-27 00:00:00

abstract：:Two affinity columns comprising immobilized concanavalin A (Con A), Con A-Sepharose and Con A-XP3507, were evaluated for their purifying ability for the glycoprotein allergen Ag7 from a partially purified extract of mugwort pollen. The most pronounced difference between the two columns was the nature of their nonspeci...

journal_title：Journal of biotechnology

authors： Nilsen BM,Smestad Paulsen B,Clonis Y,Mellbye KS

更新日期：1990-11-01 00:00:00

abstract：:2'-Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk, which is involved in many biological functions for human health. To date, most microbial systems for 2-FL production have been limited to use Escherichia coli JM strains since they cannot metabolize lactose. In this study, E. c...

journal_title：Journal of biotechnology

authors： Chin YW,Kim JY,Lee WH,Seo JH

更新日期：2015-09-20 00:00:00

abstract：:Protein engineering has been employed to investigate the effect of specific amino acid changes on the targeting of heterologous proteins to the outer cell surface of the Gram-positive bacterium Staphylococcus xylosus. Three different variants, corresponding to a 101 amino acid region of the major glycoprotein (G prote...

journal_title：Journal of biotechnology

authors： Nguyen TN,Gourdon MH,Hansson M,Robert A,Samuelson P,Libon C,Andréoni C,Nygren PA,Binz H,Uhlén M

更新日期：1995-10-16 00:00:00

abstract：:A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

journal_title：Journal of biotechnology

authors： Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

更新日期：2000-06-23 00:00:00

abstract：:One important question in microbiome analysis is how to assess the homogeneity of the microbial composition in a given environment, with respect to a given analysis method. Do different microbial samples taken from the same environment follow the same taxonomic distribution of organisms, or the same distribution of fu...

journal_title：Journal of biotechnology

authors： Huson DH,Steel M,El-Hadidi M,Mitra S,Peter S,Willmann M

更新日期：2017-05-20 00:00:00

abstract：:Saccharum spontaneum L., a wild relative of sugarcane, is known for its adaptability to environmental stresses, particularly cold stress. In the present study, an attempt was made for transcriptome profiling of the low temperature (10°C) tolerant S. spontaneum clone IND 00-1037 collected from high altitude regions of ...

journal_title：Journal of biotechnology

authors： Dharshini S,Chakravarthi M,J AN,Manoj VM,Naveenarani M,Kumar R,Meena M,Ram B,Appunu C

更新日期：2016-08-10 00:00:00

abstract：:Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

journal_title：Journal of biotechnology

authors： Chen C,Huang YL,Yang ST

更新日期：2002-07-17 00:00:00

abstract：:Zeolitic imidazole frameworks (ZIFs) with tunable pore sizes and high surface areas have recently used as an effective support for immobilizing enzymes. However, the instability in the aqueous acidic environment has limited their practical applications in some cases. In this work, we develop a novel catalase/ZIFs comp...

journal_title：Journal of biotechnology

authors： Feng Y,Zhong L,Hou Y,Jia S,Cui J

更新日期：2019-12-20 00:00:00

abstract：:Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumno rosa (PAR) was chosen as model fruit tree species and transformed with a reporter gene (uidA) ...

journal_title：Journal of biotechnology

authors： Maghuly F,da Câmara Machado A,Leopold S,Khan MA,Katinger H,Laimer M

更新日期：2007-01-01 00:00:00

abstract：:An increasing number of investors is looking at algae as a viable source of biofuels, beside cultivation for human/animal feeding or to extract high-value chemicals and pharmaceuticals. However, present biomass productivities are far below theoretical estimations implying that a large part of the available photosynthe...

journal_title：Journal of biotechnology

authors： Formighieri C,Franck F,Bassi R

更新日期：2012-11-30 00:00:00

abstract：:Vertebrate prostaglandin H synthases (PGHSs) are membrane-bound disulphide-containing hemoglycoproteins. Therefore, eukaryotic expression systems are required for the production of recombinant PGHSs. Recently we announced the expression of human PGHS-2 (hPGHS-2) in the yeast Pichia pastoris. Here we report improved pr...

journal_title：Journal of biotechnology

authors： Kukk K,Samel N

更新日期：2016-08-10 00:00:00

abstract：:An NADH-dependent phenylpyruvate reductase (LaPPR) was identified through screening the shotgun library of Lactobacillus sp. CGMCC 9967. It belongs to D-3-phosphoglycerate dehydrogenase (PGDH) subfamily of 2-hydroxy acid dehydrogenase superfamily. LaPPR was stable at pH 6.5 and 30 °C, with a half-life of 152 h. LaPPR ...

journal_title：Journal of biotechnology

authors： Xu GC,Zhang LL,Ni Y

更新日期：2016-03-20 00:00:00

abstract：:Medium composition and cultivation conditions were constructed for the optimum production of leupeptin by Streptomyces exfoliatus SMF13. The production of leupeptin was related to mycelial growth, being optimum in the cultivation with glucose-excess, phosphate-limited, and casamino acids medium. However, leupeptin-ina...

journal_title：Journal of biotechnology

authors： Kim IS,Lee KJ

更新日期：1995-08-15 00:00:00