当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Extracellular particles of polymeric material formed in n-hexadecane fermentation by Pseudomonas aeruginosa.

Extracellular particles of polymeric material formed in n-hexadecane fermentation by Pseudomonas aeruginosa.

Abstract：

:In n-hexadecane fermentation by Pseudomonas aeruginosa, the production of rhamnolipids (biosurfactants) caused by nitrogen source limitation was observed during the stationary phase. The rhamnolipids caused severe foaming in the process, particularly at higher pH (ca. > 6.8). To reduce the foaming, several runs were made at a lower pH, i.e. 6.5 +/- 0.1. Some, however, behaved anomalously different. The rhamnolipid synthesis was very low. Instead, a large quantity of waxy particles with sizes up to 3-5 mm were formed. The waxy material was practically insoluble in all of the organic solvents tested, suggesting the cross-linked polymeric structure. A white, brittle solid was obtained after the material was thoroughly washed (with 0.05 M sodium bicarbonate, distilled water, and hexane) and vacuum-dried. Characterization of the washed material was made by Fourier-transformed infrared (FTIR) spectroscopy, thin-layer chromatography (TLC), and wet chemical analyses. It was found to contain less than 2.5% (w/w) proteins and less than 9% carbohydrates, of which only one sugar component was observed and tentatively identified as glucose by the TLC analysis. The material was, therefore, predominantly hydrocarbon-based, with oxidized functional groups such as esters, alcohols, and carboxylic acids being identified by FTIR. The lipase-catalyzed esterification of fatty acids and alcohols, within the oil droplets or at the oil/water interface of low local water activity, is postulated to play an important role in the waxy particle formation.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Wu J,Ju LK

doi

10.1016/s0168-1656(97)00150-8

subject

Has Abstract

pub_date

1997-01-03 00:00:00

pages

193-202

issue

3

eissn

0168-1656

issn

1873-4863

pii

S0168165697001508

journal_volume

59

pub_type

杂志文章

相关文献

JOURNAL OF BIOTECHNOLOGY文献大全
  • Gelatin enhances 2-keto-L-gulonic acid production based on Ketogulonigenium vulgare genome annotation.

    abstract：:In the two-step fermentative production of vitamin C, its precursor 2-keto-L-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The reconstruction of the amino acid metabolic pathway through completed genome sequence annotation demonstrated that K. vulgare w...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.08.007

    authors： Liu L,Chen K,Zhang J,Liu J,Chen J

    更新日期：2011-12-10 00:00:00

  • Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins.

    abstract：:Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(01)00383-2

    authors： Bandmann N,Van Alstine J,Veide A,Nygren PA

    更新日期：2002-01-31 00:00:00

  • Heterologous production of different styrene oxide isomerases for the highly efficient synthesis of phenylacetaldehyde.

    abstract：:The styrene oxide isomerase (SOI, StyC) represents a key enzyme of the styrene-degrading pathway and has been discussed as promising biocatalyst during recent studies. The enzyme enables the synthesis of pure phenylacetaldehyde from styrene oxide. In this study the native as well as the corresponding codon-optimized g...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.04.038

    authors： Oelschlägel M,Richter L,Stuhr A,Hofmann S,Schlömann M

    更新日期：2017-06-20 00:00:00

  • Improved transgene expression fine-tuning in mammalian cells using a novel transcription-translation network.

    abstract：:Following the discovery of RNA interference (RNAi) and related phenomena, novel regulatory processes, attributable to small non-protein-coding RNAs, continue to emerge. Capitalizing on the ability of artificial short interfering RNAs (siRNAs) to trigger degradation of specific target transcripts, and thereby silence d...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.01.003

    authors： Malphettes L,Fussenegger M

    更新日期：2006-08-05 00:00:00

  • Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G.

    abstract：:The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). T...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.12.025

    authors： Schenk JA,Fettke J,Lenz C,Albers K,Mallwitz F,Gajovic-Eichelmann N,Ehrentreich-Förster E,Kusch E,Sellrie F

    更新日期：2012-03-31 00:00:00

  • Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers.

    abstract：:Cutinase from Fusarium solani pisi was genetically modified near the active site, by site-directed mutagenesis, to enhance its activity towards polyethylene terephthalate (PET) and polyamide 6,6 (PA 6,6) fibers. The mutations L81A, N84A, L182A, V184A and L189A were done to enlarge the active site in order to better fi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.12.028

    authors： Araújo R,Silva C,O'Neill A,Micaelo N,Guebitz G,Soares CM,Casal M,Cavaco-Paulo A

    更新日期：2007-03-10 00:00:00

  • Impact of transport processes in the osmotic response of Corynebacterium glutamicum.

    abstract：:Osmoregulation, the adaptation of cells to changes in the external osmolarity, is an important aspect of the bacterial stress response, in particular for a soil bacterium like Corynebacterium glutamicum. Consequently, this organism is equipped with several redundant systems for coping with both hyper- and hypoosmotic ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1656(03)00164-0

    authors： Morbach S,Krämer R

    更新日期：2003-09-04 00:00:00

  • A comparative study of structure, stability and function of sc-tenecteplase in the presence of stabilizing osmolytes.

    abstract：:The aim of the present study was to investigate the effect of three routine drug excipients, as osmolytes, in three different concentrations, on structure, thermal stability and the activity of single-chain (sc-) tenecteplase. To see the influence of trehalose, mannitol, and sucrose on the structure, stability and func...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2018.05.014

    authors： Bayat M,Gourabi H,Khammari A,Ahmad F,Saboury AA

    更新日期：2018-08-20 00:00:00

  • Wafer-scale nanowell array patterning based electrochemical impedimetric immunosensor.

    abstract：:We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.08.029

    authors： Lee J,Cho S,Lee J,Ryu H,Park J,Lim S,Oh B,Lee C,Huang W,Busnaina A,Lee H

    更新日期：2013-12-01 00:00:00

  • A new arylesterase from Pseudomonas pseudoalcaligenes can hydrolyze ionic phthalic polyesters.

    abstract：:Extracellular enzymes are assumed to be responsible for the initial and rate limiting step in biodegradation of polymers. Mainly enzymes with aliphatic esters as their natural substrates (e.g. lipase, cutinases) have until now been evaluated for polyester hydrolysis studies. However, the potential of enzymes with arom...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.01.012

    authors： Haernvall K,Zitzenbacher S,Yamamoto M,Schick MB,Ribitsch D,Guebitz GM

    更新日期：2017-09-10 00:00:00

  • Histamine dehydrogenase from Rhizobium sp.: gene cloning, expression in Escherichia coli, characterization and application to histamine determination.

    abstract：:The gene encoding histamine dehydrogenase in Rhizobium sp. 4--9 has been cloned and overexpressed in Escherichia coli. The coding region of the gene was 2,079 bp and encoded a protein of 693 amino acids with a calculated molecular mass of 76,732 Da. This histamine dehydrogenase was related to histamine dehydrogenase f...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.04.005

    authors： Bakke M,Sato T,Ichikawa K,Nishimura I

    更新日期：2005-09-29 00:00:00

  • A three-step strategy for targeting drug carriers to human ovarian carcinoma cells in vitro.

    abstract：:To improve tumor-to-tissue ratios of anticancer agents in radioimmunotherapy, a three-step targeting approach was used to deliver biotinylated liposomes to human ovarian cancer cells (NIH:OVCAR-3, SK-OV-3) in vitro. Targeting was based upon the use of two antibodies specific for the CA-125 antigen that is highly expre...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(01)00424-2

    authors： Xiao Z,McQuarrie SA,Suresh MR,Mercer JR,Gupta S,Miller GG

    更新日期：2002-03-28 00:00:00

  • High level production of bioactive di- and tri-tyrosine peptides by protease-catalyzed reactions.

    abstract：:Papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media was efficient for synthesis of oligo-tyrosine peptides having angiotensin I-converting enzyme inhibitory activity. Di-, tri-, and tetra-tyrosine accumulated in the soluble fraction of reaction mixture. On the other hand, the peptide products wi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2010.09.931

    authors： Narai-Kanayama A,Shikata Y,Hosono M,Aso K

    更新日期：2010-11-01 00:00:00

  • Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099.

    abstract：:Two extra-cellular endoxylanases (Xyl Ia and Ib) were purified to homogeneity from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Xyl Ia and Ib, having a molecular mass of approximately 53 kDa and pI of 5.2 and 4.8, respectively, were optimally active at 75 degrees C and at pH 6.0. They were st...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2003.12.010

    authors： Chadha BS,Ajay BK,Mellon F,Bhat MK

    更新日期：2004-04-29 00:00:00

  • α-Chymotrypsin-catalyzed synthesis of poly-l-cysteine in a frozen aqueous solution.

    abstract：:Poly-l-cysteine (PLCys) is drawing attention as a potential sorbent of thiol (SH)-reactive toxic heavy metal ions in the wastewater and polluted soils. However, preparation of PLCys relies on chemically synthesized polymers, in which SH groups must be protected and deprotected prior to use. On the other hand, α-chymot...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.12.021

    authors： Narai-Kanayama A,Hanaishi T,Aso K

    更新日期：2012-02-10 00:00:00

  • High-yield production of D-1,2,4-butanetriol from lignocellulose-derived xylose by using a synthetic enzyme cascade in a cell-free system.

    abstract：:Approaches using metabolic engineering to produce D-1, 2, 4-butanetriol (BT) from renewable biomass in microbial systems have achieved initial success. However, due to the lack of incomplete understanding of the complex branch pathway, the efficient fermentation system for BT production was difficult to develop. Here ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2019.01.004

    authors： Gao Q,Wang X,Hu S,Xu N,Jiang M,Ma C,Yang J,Xu S,Chen K,Ouyang P

    更新日期：2019-02-20 00:00:00

  • Characterization of the promoter and upstream activating sequence from the Pseudomonas alcaligenes lipase gene.

    abstract：:Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00397-7

    authors： Cox M,Gerritse G,Dankmeyer L,Quax WJ

    更新日期：2001-03-09 00:00:00

  • Purification, characterization, and synergistic action of phytate-resistant alpha-amylase and alpha-glucosidase from Geobacillus thermodenitrificans HRO10.

    abstract：:The alpha-amylase (1, 4-alpha-d-glucanohydrolase; EC 3.2.1.1) and alpha-glucosidase (alpha-d-glucoside glucohydrolase; EC 3.2.1.20) secreted by Geobacillus thermodenitrificans HRO10 were purified to homogeneity (13.6-fold; 11.5% yield and 25.4-fold; 32.0% yield, respectively) through a series of steps. The molecular w...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.02.006

    authors： Ezeji TC,Bahl H

    更新日期：2006-08-20 00:00:00

  • The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology.

    abstract：:Composition and gene content of a biogas-producing microbial community from a production-scale biogas plant fed with renewable primary products was analysed by means of a metagenomic approach applying the ultrafast 454-pyrosequencing technology. Sequencing of isolated total community DNA on a Genome Sequencer FLX Syst...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2008.05.008

    authors： Schlüter A,Bekel T,Diaz NN,Dondrup M,Eichenlaub R,Gartemann KH,Krahn I,Krause L,Krömeke H,Kruse O,Mussgnug JH,Neuweger H,Niehaus K,Pühler A,Runte KJ,Szczepanowski R,Tauch A,Tilker A,Viehöver P,Goesmann A

    更新日期：2008-08-31 00:00:00

  • Synthesis and characterization of a new cutinase substrate, 4-nitrophenyl (16-methyl sulfone ester) hexadecanoate.

    abstract：:Phytopathogenic fungi penetrate plants by breaking down the cuticular barrier with cutinase. Cutinases are extracellular hydrolytic enzymes that degrade cutin, a polyester composed of hydroxy and epoxy fatty acids. Until now, cutinase has been recognized by its ability to release labeled cutin monomers or by a non-spe...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.08.011

    authors： Degani O,Salman H,Gepstein S,Dosoretz CG

    更新日期：2006-02-10 00:00:00

  • Continuous production of lactulose by immobilized thermostable beta-glycosidase from Pyrococcus furiosus.

    abstract：:A continuous enzymatic process for the production of the prebiotic disaccharide lactulose through transgalactosylation was developed using free and immobilized beta-glycosidase from Pyrococcus furiosus. The hyperthermostable beta-glycosidase (CelB) was immobilized onto an anion-exchange resin (Amberlite IRA-93) or ont...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2009.12.017

    authors： Mayer J,Kranz B,Fischer L

    更新日期：2010-02-15 00:00:00

  • Clavibacter michiganensis subsp. michiganensis: first steps in the understanding of virulence of a Gram-positive phytopathogenic bacterium.

    abstract：:Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. The wild-type strain NCPPB382 carries two plasmids, pCM1 and pCM2. The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no dise...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jbiotec.2003.07.011

    authors： Gartemann KH,Kirchner O,Engemann J,Gräfen I,Eichenlaub R,Burger A

    更新日期：2003-12-19 00:00:00

  • Antibiotic resistance in Pseudomonas aeruginosa biofilms: towards the development of novel anti-biofilm therapies.

    abstract：:The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilm...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jbiotec.2014.09.003

    authors： Taylor PK,Yeung AT,Hancock RE

    更新日期：2014-12-10 00:00:00

  • Pimprinine, an extracellular alkaloid produced by Streptomyces CDRIL-312: fermentation, isolation and pharmacological activity.

    abstract：:Pimprinine, an extracellular alkaloid has been isolated from the culture filtrate of Streptomyces CDRIL-312. Pimprinine was subsequently purified using silica gel column chromatography and also by preparatory thin layer chromatography. Some physicochemical properties, antimicrobial activities (in-vitro) and pharmacolo...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(01)00244-9

    authors： Naik SR,Harindran J,Varde AB

    更新日期：2001-06-01 00:00:00

  • Effects of purified endo-beta-1,4-xylanases of family 10 and 11 and acetyl xylan esterases on eucalypt sulfite dissolving pulp.

    abstract：:Sulfite dissolving pulp from Eucalyptus grandis contained approximately 3.8% O-acetyl-4-O-methylglucuronoxylan with a molar ratio of xylose:4-O-methylglucuronic acid:acetyl group close to 13.6:1:6.2. The effects produced by purified endo-xylanases from two different glycosyl hydrolase families (family 10 and 11) as we...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00324-2

    authors： Christov L,Biely P,Kalogeris E,Christakopoulos P,Prior BA,Bhat MK

    更新日期：2000-10-13 00:00:00

  • Solid-phase genetic engineering with DNA immobilized on a gold surface.

    abstract：:A novel method for immobilizing large DNA fragments on a solid surface was developed. A mixed self-assembled monolayer of thiolated single-stranded DNA with inert alkanethiol was generated on a gold (Au) surface through the Au-S reaction. Surface-tethered DNA generated by this method was compatible with various geneti...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(02)00051-2

    authors： Kim JH,Hong JA,Yoon M,Yoon MY,Jeong HS,Hwang HJ

    更新日期：2002-07-03 00:00:00

  • Differentiation of five tuna species by a multiplex primer-extension assay.

    abstract：:A novel methodology based on analysis of mtDNA-cytb diagnostic sites was performed to discriminate four closely related species of Thunnus (Thunnus alalunga, Thunnus albacares, Thunnus obesus and Thunnus thynnus) and one species of Euthynnus (Katsuwonus pelamis) genus in raw and canned tuna. The primers used in the pr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.01.032

    authors： Bottero MT,Dalmasso A,Cappelletti M,Secchi C,Civera T

    更新日期：2007-05-01 00:00:00

  • Dynamic metabolic response of Aspergillus niger to glucose perturbation: evidence of regulatory mechanism for reduced glucoamylase production.

    abstract：:Environmental gradient is an important common issue during scale-up process for protein production. To address the dynamic regulatory mechanism of Aspergillus niger being exposed to inhomogeneous glucose concentrations, glucose perturbation were experimented on the steady state of A. niger chemostat culture, and dynam...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2018.08.005

    authors： Li C,Shu W,Wang S,Liu P,Zhuang Y,Zhang S,Xia J

    更新日期：2018-12-10 00:00:00

  • Stability of alpha-amylase immobilized on poly(methyl methacrylate-acrylic acid) microspheres.

    abstract：:Poly(methyl methacrylate-acrylic acid) microspheres were prepared and the acid groups were activated by using either carbodiimide (CDI) or thionyl chloride (SOCl2). alpha-Amylase was covalently bound on these activated microspheres. The properties of the immobilized enzyme were investigated and compared with those of ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(97)00179-x

    authors： Aksoy S,Tumturk H,Hasirci N

    更新日期：1998-02-05 00:00:00

  • Complete genome sequence of Enterobacter cloacae GGT036: a furfural tolerant soil bacterium.

    abstract：:Enterobacter cloacae is a facultative anaerobic bacterium to be an important cause of nosocomial infection. However, the isolated E. cloacae GGT036 showed higher furfural-tolerant cellular growth, compared to industrial relevant strains such as Escherichia coli and Corynebacterium glutamicum. Here, we report the compl...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.11.012

    authors： Gong G,Um Y,Park TH,Woo HM

    更新日期：2015-01-10 00:00:00