Pimprinine, an extracellular alkaloid produced by Streptomyces CDRIL-312: fermentation, isolation and pharmacological activity.

abstract：:Type 2 diabetes, characterized by peripheral target tissue resistance to insulin, is epidemic in industrialized countries and is strongly associated with obesity. The protein hormone, resistin, secreted specifically by the adipose tissues, is found to antagonize insulin action upon glucose uptake and may serve as an i...

journal_title：Journal of biotechnology

authors： Juan CC,Kan LS,Huang CC,Chen SS,Ho LT,Au LC

更新日期：2003-06-26 00:00:00

abstract：:Aptamers are single-stranded DNA or RNA oligonucleotides, which have been emerging as recognition elements in disease diagnostics and food control, including the detection of bacterial toxins. In this study, we employed the semi-automated just in time-selection to identify aptamers that bind to cholera toxin (CT) with...

journal_title：Journal of biotechnology

authors： Frohnmeyer E,Frisch F,Falke S,Betzel C,Fischer M

更新日期：2018-03-10 00:00:00

abstract：:New morphological aspects of Penicillium chrysogenum were found during physiological characterisation of two NADPH-dependent glutamate dehydrogenase mutant strains. A morphological characterisation of the previously constructed strains, together with the two beta-lactam producing industrial recipient strains, was cond...

journal_title：Journal of biotechnology

authors： Thykaer J,Rueksomtawin K,Noorman H,Nielsen J

更新日期：2009-02-23 00:00:00

abstract：:Heparan sulfate (HS) is a cell surface carbohydrate polymer modified with sulfate moieties whose highly ordered composition is central to directing specific cell signaling events. The ability of the cell to generate these information rich glycans with such specificity has opened up a new field of "heparanomics" which ...

journal_title：Journal of biotechnology

authors： Lamanna WC,Kalus I,Padva M,Baldwin RJ,Merry CL,Dierks T

更新日期：2007-04-30 00:00:00

abstract：:This paper discusses the concept of generic model-based software sensors with particular reference to bioprocess application. The industrial need for software sensing is considered and two industrial bioprocess systems are used in order to highlight the operability problems which warrant their development. Alternative...

journal_title：Journal of biotechnology

authors： Montague GA,Morris AJ,Tham MT

更新日期：1992-08-01 00:00:00

abstract：:Physiologically relevant in vitro models can serve as biological analytical platforms for testing novel treatments and drug delivery systems. We describe the first steps in the development of a 3D human brain tumour co-culture model that includes the interplay between normal and tumour tissue along with nutrient gradi...

journal_title：Journal of biotechnology

authors： Ivanov DP,Parker TL,Walker DA,Alexander C,Ashford MB,Gellert PR,Garnett MC

更新日期：2015-07-10 00:00:00

abstract：:A Gram-negative, rod-shaped, non-motile, gamma and UV radiation resistant bacterium Hymenobacter radioresistens DG25B was isolated from a soil sample collected in South Korea. The complete genome sequence of H. radioresistens DG25B consists of one circular chromosome (3,874,646 bp). The bacterium was isolated from gam...

journal_title：Journal of biotechnology

authors： Kim MK,Joo ES,Lee SY,Lee DS,Srinivasan S,Jung HY

更新日期：2016-01-10 00:00:00

abstract：:The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant. When transgen...

journal_title：Journal of biotechnology

authors： Kang TJ,Lee WS,Choi EG,Kim JW,Kim BG,Yang MS

更新日期：2006-01-24 00:00:00

abstract：:A novel method for immobilizing large DNA fragments on a solid surface was developed. A mixed self-assembled monolayer of thiolated single-stranded DNA with inert alkanethiol was generated on a gold (Au) surface through the Au-S reaction. Surface-tethered DNA generated by this method was compatible with various geneti...

journal_title：Journal of biotechnology

authors： Kim JH,Hong JA,Yoon M,Yoon MY,Jeong HS,Hwang HJ

更新日期：2002-07-03 00:00:00

abstract：:The development of SIN-lentivectors has paved the way for the double copy vectors (DCV), which substitute the deletion in the 3'LTR with either transgenic or insulator sequences. However, the limits of this approach remain unclear. Previous results have demonstrated that transduction efficiencies of DCV carrying large...

journal_title：Journal of biotechnology

authors： Bos TJ,De Bruyne E,Van Lint S,Heirman C,Vanderkerken K

更新日期：2010-10-01 00:00:00

abstract：:Extracellular enzymes are assumed to be responsible for the initial and rate limiting step in biodegradation of polymers. Mainly enzymes with aliphatic esters as their natural substrates (e.g. lipase, cutinases) have until now been evaluated for polyester hydrolysis studies. However, the potential of enzymes with arom...

journal_title：Journal of biotechnology

authors： Haernvall K,Zitzenbacher S,Yamamoto M,Schick MB,Ribitsch D,Guebitz GM

更新日期：2017-09-10 00:00:00

abstract：:Superoxide dismutases are ubiquitous enzymes which play an important role in protecting cells against oxidative damage and which have also been shown to contribute to the pathogenicity of many bacterial species. Here we demonstrate that Vibrio cholerae, the causative agent of cholerae, expresses an active periplasmic ...

journal_title：Journal of biotechnology

authors： Gabbianelli R,Signoretti C,Marta I,Battistoni A,Nicolini L

更新日期：2004-04-08 00:00:00

abstract：:Inoculation of naked DNA has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. To study the humoral immune response induced by DNA vaccination we cloned the entire nucleocapsid protein-encoding sequence of the Puumala hantavirus strain Vranica/Hällnäs into th...

journal_title：Journal of biotechnology

authors： Koletzki D,Schirmbeck R,Lundkvist A,Meisel H,Krüger DH,Ulrich R

更新日期：2001-11-17 00:00:00

abstract：:The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30...

journal_title：Journal of biotechnology

authors： Ouyang J,Wang X,Zhao B,Yuan X,Wang Y

更新日期：2003-04-24 00:00:00

abstract：:Most expression systems used for the over production of many enzymes employ carbon catabolite repressible promoters and hence must use sugar free, rich complex media. Use of expression systems to overcome carbon repression opens an avenue for exploiting cheap carbon sources for the production of recombinant enzymes. A...

journal_title：Journal of biotechnology

authors： Nathan S,Nair M

更新日期：2013-12-01 00:00:00

abstract：:In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based monoclonal antibody (mAb) affinity precipitation process is developed using scaled-down filtration techniques. Tangential flow filtration (TFF) is examined for the recovery of ELP-Z-mAb precipitates formed during the mAb binding st...

journal_title：Journal of biotechnology

authors： Sheth RD,Bhut BV,Jin M,Li Z,Chen W,Cramer SM

更新日期：2014-12-20 00:00:00

abstract：:This study was performed to determine if Akebia and Aristolochia species (Akebia quinata Decaisne and Aristolochia manshuriensis Kom.) could be identified by genetic analysis of Pyrosequencing method, which is used to assess genetic variation. The Pyrosequencing results of Akebia and Aristolochia species showed differ...

journal_title：Journal of biotechnology

authors： Han S,Kim S,Yang CH,Seo J

更新日期：2005-12-06 00:00:00

abstract：:Two-thirds of Earth's surface is covered by oceans, yet the study of this massive integrated living system is still in its infancy. Various environmental variables, such as high salinity, low and changeable nutrient availability and depth-correlated gradients of light, temperature, nutrients and pressure shape the div...

journal_title：Journal of biotechnology

authors： Simonato F,Campanaro S,Lauro FM,Vezzi A,D'Angelo M,Vitulo N,Valle G,Bartlett DH

更新日期：2006-10-20 00:00:00

abstract：:Each of the twelve enzymes for glycolytic fermentation, eleven from Escherichia coli and one from Saccharomyces cerevisiae, have been over-expressed in E. coli and purified with His-tags. Simple assays have been developed for each enzyme and they have been assembled for fermentation of glucose to ethanol. Phosphorus-3...

journal_title：Journal of biotechnology

authors： Stevenson BJ,Liu JW,Kuchel PW,Ollis DL

更新日期：2012-01-01 00:00:00

abstract：:Mutants of transketolase (TK) with improved substrate specificity towards the non-natural aliphatic aldehyde substrate propionaldehyde have been obtained by directed evolution. We used the same active-site targeted saturation mutagenesis libraries from which we previously identified mutants with improved activity towa...

journal_title：Journal of biotechnology

authors： Hibbert EG,Senussi T,Smith ME,Costelloe SJ,Ward JM,Hailes HC,Dalby PA

更新日期：2008-04-30 00:00:00

abstract：:Many recent epigenetic studies utilize the advantages of CRISPR/dCas9 based tools in linking certain epigenetic modification with gene expression regulation. Various multifactorial diseases often contain changed epigenetic signatures at many loci, so tools for simultaneously targeting different loci would significantl...

journal_title：Journal of biotechnology

authors： Josipović G,Zoldoš V,Vojta A

更新日期：2019-08-10 00:00:00

abstract：:Vacuolar sequestration of valuable secondary metabolites remains the major limitation to the use of immobilization technology for large scale plant-cell-based bioprocesses, which otherwise may be a more efficient culture system than suspension for this biomass. In this initial study, the release of indole alkaloids pr...

journal_title：Journal of biotechnology

authors： Jardin B,Tom R,Chavarie C,Rho D,Archambault J

更新日期：1991-11-01 00:00:00

abstract：:We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In...

journal_title：Journal of biotechnology

authors： Lee J,Cho S,Lee J,Ryu H,Park J,Lim S,Oh B,Lee C,Huang W,Busnaina A,Lee H

更新日期：2013-12-01 00:00:00

abstract：:Chimeric virus-like particles (VLP) are known as promising tools in the development of safe and effective subunit vaccines. Recently, a technology platform to produce VLP based on the small surface protein (dS) of the duck hepatitis B virus was established. In this study, chimeric VLP were investigated displaying the ...

journal_title：Journal of biotechnology

authors： Wetzel D,Barbian A,Jenzelewski V,Schembecker G,Merz J,Piontek M

更新日期：2019-12-20 00:00:00

abstract：:Deracemization of D,L-phosphinothricin (D,L-PPT) is one of the most promising routes for preparation of optically pure L-PPT. In this work, an efficient multi-enzyme redox cascade was developed for deracemization ofPPT, which includes oxidative reaction and reductive reaction. The oxidative reaction catalyzing oxidati...

journal_title：Journal of biotechnology

authors： Cao CH,Gong H,Dong Y,Li JM,Cheng F,Xue YP,Zheng YG

更新日期：2021-01-10 00:00:00

abstract：:Rebuilding enzymes with high activities, stability and reusability has been constantly pursued for application of enzymaticcatalysis in wide field. Rebuilding enzymes by chemical approaches, including immobilization and chemicalmodification, represents a simple but effective route. Here, a class of highly stable and a...

journal_title：Journal of biotechnology

authors： Li L,Li H,Yan B,Yu S

更新日期：2019-02-10 00:00:00

abstract：:Secreted recombinant proteins can aggregate during cell culture. We studied a poorly-behaved bispecific scaffold that increasingly aggregated (up to 62% high molecular weight species, HMW) as a function of culture time in a fed-batch and intensified cell culture processes. We identified that protein aggregates increas...

journal_title：Journal of biotechnology

authors： Gomez N,Barkhordarian H,Lull J,Huh J,GhattyVenkataKrishna P,Zhang X

更新日期：2019-10-10 00:00:00

abstract：:Proteolytically cleaved human 22 kDa growth hormone (22K hGH) between the amino acid residues 134 and 150 by plasmin or other proteases in vitro has been reported to be most active in growth promoting activity. In this study a deleted mutant hGH lacking amino acid residues from 135 to 146 and having more sensitivity t...

journal_title：Journal of biotechnology

authors： Alam KS,Fujikawa T,Yoshizato H,Tanaka M,Nakashima K

更新日期：2000-02-28 00:00:00

abstract：:The genome of the biotechnologically important solventogenic Clostridium saccharobutylicum NCP 262 contains two operons coding for genes of presumed type I RM systems belonging to the families A and C. They represent a limiting factor for the development of transformation and conjugation protocols. We established an e...

journal_title：Journal of biotechnology

authors： Lesiak JM,Liebl W,Ehrenreich A

更新日期：2014-10-20 00:00:00

abstract：:This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene ex...

journal_title：Journal of biotechnology

authors： Sproat BS

更新日期：1995-07-31 00:00:00